Abstract
Single spore derived isolates of Plasmodiophora brassicae are difficult to be generated due to its biotrophic life style. Two methods to produce pure pathotype isolates of P. brassicae were compared. A new technique for the inoculation of Brassica seedlings with single root hairs infected by P. brassicae is described, which is taking advantage of the multiplication of P. brassicae during the first infection cycle in root hairs. Ten single spore derived isolates from 328 single sporangiosorus inoculations were produced using different populations of P. brassicae. To compare success rates inoculations with single resting spores were also made. From 125 inoculations with single resting spores only one single spore derived isolate was achieved. The root hair inoculation method was more practicable than most hitherto reported methods and offers a reasonable efficacy for the production of single spore derived isolates.
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References
Arbeiter, A., Fähling, M., Graf, H., Sacristán, M.-D., & Siemens, J. (2003). Resistance of Arabidopsis thaliana to the obligate biotrophic parasite Plasmodiophora brassicae. Plant Protection Science, 38, 519–522.
Ayers, G. W. (1944). Studies of the life history of the clubroot organism, Plasmodiophora brassicae. Canadian Journal of Research, 22, 143–149.
Buczacki, S. T. (1977). Root infections from single resting spores of Plasmodiophora brassicae. Transactions of the British Mycological Society, 69, 328–329.
Buczacki, S. T., Toxopeus, H., Mattusch, P., Johnston, T. D., Dixon, G. R., & Hobolth, L. A. (1975). Study of physiologic specialization in Plasmodiophora brassicae: proposals for attempted rationalization through an international approach. Transactions of the British Mycological Society, 65, 295–303.
Cook, W. R. I., & Schwartz, E. J. (1930). The life-history, cytology and method of infection of Plasmodiophora brassicae Wor., the cause of the finger-and-toe disease of cabbages and other crucifers. Philosophical Transactions of the Royal Society of London, Series B, 218, 283–314.
Crute, I. R., Gray, A. R., Crisp, P., & Buczacki, S. T. (1980). Variation in Plasmodiophora brassicae and resistance to clubroot disease in Brassicas and allied crops - A critical review. Plant Breeding Abstracts, 50, 91–104.
Diederichsen, E., & Sacristan, M. D. (1996). Disease response of resynthesized Brassica napus L. lines carrying different combinations of resistance to Plasmodiophora brassicae Wor. Plant Breeding, 115, 5–10.
Diederichsen, E., Frauen, M., Linders, E. G. A., Hatakeyama, K., & Hirai, M. (2009). Status and perspectives of clubroot resistance breeding in crucifer crops. Journal of Plant Growth Regulation, 28, 265–281.
Fähling, M., Graf, H., & Siemens, J. (2004). Characterization of a single spore isolate population of Plasmodiophora brassicae resulting from a single club. Journal of Phytopathology, 152, 438–444.
Gabrielson, R. L., & Robak, J. (1988). Temperature sensitivity of resistance to two pathotypes of Plasmodiophora brassicae in Brassica oleracea. Acta Agrobotanica, 41, 237–243.
Graf, H., Sokolowski, F., Klewer, A., Diederichsen, E., Luerssen, H., & Siemens, J. (2001). Electrophoretic karyotype of the obligate biotrophic parasite Plasmodiophora brassicae Wor. Journal of Phytopathology, 149, 313–318.
Hopf, E., Besch, M., Diederichsen, E. (1994). Influence of selective host passages on virulence patterns of a Plasmodiophora brassicae field isolate. ISHS Symposium on Brassicas. Ninth Crucifer Genetics Workshop. Lisbon, Portugal. Abstract 89.
Ingram, D. S., & Tommerup, I. C. (1972). The life history of Plasmodiophora brassicae Wor. Proceedings of the Royal Society of London, 80, 103–112.
Jones, D. R., Ingram, D. S., & Dixon, G. R. (1982a). Factors affecting tests for differential pathogenicity in populations of Plasmodiophora brassicae. Plant Pathology, 31, 229–238.
Jones, D. R., Ingram, D. S., & Dixon, G. R. (1982b). Characterization of isolates derived from single resting spores of Plasmodiophora brassicae and studies of their interaction. Plant Pathology, 31, 239–246.
Jubault, M., Lariagon, C., Simon, M., Delourme, R., & Manzanares-Dauleux, M. J. (2008). Identification of quantitative trait loci controlling partial clubroot resistance in new mapping populations of Arabidopsis thaliana. Theor Applied Genetics, 117, 191–202.
Kageyama, K., & Asano, T. (2009). Life cycle of Plasmodiophora brassicae. Journal of Plant Growth Regulation, 28, 203–211. doi:10.1007/S00344-009-9101-Z.
Karling, J. S. (1968). The Plasmodiophorales. New York: Hafner Publishing Company.
Kuginuki, Y., Yoshikawa, H., & Yui, S. (1991). Degradation of clubroot resistance in chinese cabbage - effect of temperature and day-length. Cruciferae Newsletter, 14–15, 140–141.
Manzanares, M. J., Laurens, F., Baron, F., & Thomas, G. (1994). Production of single spore isolates of Plasmodiophora brassicae. Cruciferae Newsletter, 16, 132–133.
Schoeller, M. (1987). Untersuchungen zur Resistenz von Kulturcruciferen gegen den Erreger der Kohlhernie, Plasmodiophora brassicae Wor. Hannover, Germany: Universität Hannover, Ph.D. thesis.
Schwelm, A., Fogelqvist, J., Knaust, A., Jülke, S., Lilja, T., Bonilla-Rosso, G., et al. (2015). The Plasmodiophora brassicae genome reveals insights in its life cycle and ancestry of chitin synthases. Scientific Reports, Article: 11153, doi:10.1038/srep11153
Scott, E. S. (1985). Production and characterization of single-spore isolates of Plasmodiophora brassicae. Plant Pathology, 34, 287–292.
Tinggal, S. H., & Webster, J. (1981). Technique for single spore infection by Plasmodiophora brassicae. Transactions of the British Mycological Society, 76, 187–190.
Tommerup, I. C., & Ingram, D. S. (1971). The life-cycle of Plasmodiophora brassicae Woron. in Brassica tissue cultures and in intect roots. New Phytol, 70, 327–332.
Toxopeus, H., Dixon, G. R., & Mattusch, P. (1986). Physiological specialization in Plasmodiophora brassicae: an analysis by international experimentation. Transactions of the British Mycological Society, 87, 279–287.
Xue, S., Cao, T., Howard, R. J., Hwang, S. F., & Strelkov, S. E. (2008). Isolation and variation in virulence of single-spore isolates of Plasmodiophora brassicae from Canada. Plant Disease, 92, 456–462.
Acknowledgments
The authors gratefully acknowledge the support from M.D. Sacristán for the realisation of this study and J. Siemens for helping with the manuscript.
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Diederichsen, E., Wagenblatt, B. & Schallehn, V. Production of pure genotype isolates of Plasmodiophora brassicae Wor. - comparison of inoculations with root hairs containing single sporangiosori or with single resting spores. Eur J Plant Pathol 145, 621–627 (2016). https://doi.org/10.1007/s10658-016-0876-z
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DOI: https://doi.org/10.1007/s10658-016-0876-z