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Phenotypic and genotypic responses of chili (Capsicum annuum L.) progressive lines with different resistant genes against anthracnose pathogen (Colletotrichum spp.)

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Abstract

Anthracnose disease caused by Colletotrichum spp. is a serious disease of chili (Capsicum annuum), particularly in tropical countries. Host plant resistance breeding is one of the most effective disease management strategies. It requires identification of parents with genes resistant to Colletotrichum species predominant in a given region. For phenotypic evaluation, 35 chili lines consisting of progressive lines of C. annuum derived from C. baccatum PBC80 (34 lines) and from C. chinense PBC932 (one line) were inoculated with two aggressive isolates of Colletotrichum acutatum (Ca) and C. capsici (Cc) by injection into the pericarp of green and red-ripe fruit. Lesion diameters were scored at seven days after inoculation. Two simple sequence repeat (SSR) and one sequence characterized amplified region (SCAR) markers were used for validation in four progressive lines and three each of susceptible and resistant checks for genotypic response. The progressive lines were classified into 10 groups based on their responses to two pathogens and at the two fruit stages. Four progressive lines (101, 205, 210 and 215) were selected and used for developing crosses to combine resistance genes from two sources resistant to Ca and Cc at both fruit stages. Progressive lines derived from PBC80 showed DNA fragment 231 bp amplified by primer HpmsE032 associated with Cc at green fruit stages. Hence the HpmsE032 marker could be considered useful in the selection of resistant genotypes derived from PBC80.

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Acknowledgments

The authors are grateful to The Royal Golden Jubilee (RGJ) Ph.D. Program, Thailand Research Fund (TRF); The National Science and Technology Agency (NSTDA); National Research University Project of Thailand, Office of the Higher Education Commission, through the Food and Functional Food Research Cluster of Khon Kaen University; Plant Breeding Research Center for Sustainable Agriculture, Khon Kaen University for funding the research, Thailand Research Fund(TRF) (Project code : IRG5780003), Khon Kaen University (KKU) and the Faculty of Agriculture KKU for providing financial support for manuscript preparation activities and RGJ fellowship to the first author. The authors are also grateful to AVRDC – The World Vegetable Center, Taiwan and Asst. Prof. Chalermsri Nontaswatsri, Maejo University, Thailand for providing seeds of genotypes.

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Authors and Affiliations

  1. Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand

    Patcharaporn Suwor, Petcharat Thummabenjapone, Jirawat Sanitchon & Suchila Techawongstien

  2. AVRDC – The World Vegetable Center, P.O. Box 42, Shanhua, Tainan, 74199, Taiwan

    Sanjeet Kumar

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  1. Patcharaporn Suwor
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  2. Petcharat Thummabenjapone
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  3. Jirawat Sanitchon
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  4. Sanjeet Kumar
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  5. Suchila Techawongstien
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Correspondence to Suchila Techawongstien.

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Suwor, P., Thummabenjapone, P., Sanitchon, J. et al. Phenotypic and genotypic responses of chili (Capsicum annuum L.) progressive lines with different resistant genes against anthracnose pathogen (Colletotrichum spp.). Eur J Plant Pathol 143, 725–736 (2015). https://doi.org/10.1007/s10658-015-0723-7

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  • DOI: https://doi.org/10.1007/s10658-015-0723-7

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