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Identification and species status of the mango biotype of Colletotrichum gloeosporioides in Ghana

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Abstract

Research work was carried out to identify and ascertain the species status of the mango biotype of Colletotrichum gloeosporioides infecting mangoes in Ghana. Forty five isolates of Colletotrichum species were collected from 12 districts in Ghana while five each were obtained from mango fruits from Florida, Mexico and Puerto Rico. The entire internal transcribed spacer region, partial beta-tubulin gene and partial glyceraldehyde-3-phosphate dehydrogenase gene of isolates were sequenced and used in phylogenetic studies. The results of the sequence analysis of the first ribosomal transcribed spacer (ITS 1) region showed that 35 % of the isolates from Ghana and all the five isolates from Mexico were the mango biotype of C. gloeosporioides, while the others were not. Phylogenetic studies showed that the mango biotype of the pathogen was Colletotrichum asianum but not C. gloeosporioides as previously thought. However, the other isolates that were not the mango biotype were identified as Colletotrichum siamense and Colletotrichum species which had probably cross-infected mango from other fruit crops in the field.

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Acknowledgment

The authors wish to extend their gratitude to Dr. Frank Louws of The Plant Pathology Department of North Carolina State University, The office of International Programmes (University of Ghana and North Carolina State University), Students Study Abroad Office (both Ghana and U.S.A.) and The Office of Research and Innovation Development of the University of Ghana for their diverse supports to the study.

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Authors and Affiliations

  1. Soil and Irrigation Research Centre, University of Ghana, P.O. Box LG44, Legon-Accra, Ghana

    Joseph O. Honger

  2. Biotechnology Centre, University of Ghana, P.O. Box LG44, Legon-Accra, Ghana

    Samuel K. Offei

  3. Crop Science Department, University of Ghana, P.O. Box LG44, Legon-Accra, Ghana

    Kwadwo A. Oduro & Seloame Tatu Nyaku

  4. Botany Department, University of Ghana, P.O. Box LG44, Legon-Accra, Ghana

    George T. Odamtten

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  1. Joseph O. Honger
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Correspondence to Joseph O. Honger.

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Honger, J.O., Offei, S.K., Oduro, K.A. et al. Identification and species status of the mango biotype of Colletotrichum gloeosporioides in Ghana. Eur J Plant Pathol 140, 455–467 (2014). https://doi.org/10.1007/s10658-014-0480-z

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