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Differentiation of the closely related species, Alternaria solani and A. tomatophila, by molecular and morphological features and aggressiveness

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Abstract

The main causal agent of early blight, the noxious disease of solanaceous crops, is generally considered to be Alternaria solani Sorauer (in a broad sense). However, heterogeneity in many morphological features of this pathogen has been noted suggesting that the disease may not be caused by a single species. Recent research has revealed that several large-spored Alternaria species may cause disease of potato and tomato including A. solani sensu stricto and A. tomatophila. The goal of our research was to compare Russian large-spored Alternaria isolates from tomato and potato to test the hypothesis that early blight of tomato and potato are caused by different species. Cluster analysis of genetic distances estimated from 12 polymorphic molecular markers (universally primed-polymerase chain reaction and randomly amplified polymorphic DNA) revealed two groups of isolates accepted here as A. solani and A. tomatophila that were supported by morphology and host plant association. Differentiation of species was supported by phylogeny derived from the DNA sequences of a portion of the Alt a1, gpd and calmodulin genes. Species-specific primers based on the Alt a1 and calmodulin gene sequences for both species were designed. Under laboratory conditions, A. solani isolates were equally aggressive on both tomato and potato, whereas A. tomatophila was highly aggressive to tomato but only weakly aggressive to potato. In the field, A. solani was isolated from potato, tomato and from several wild potato species including S. schickii, S. papita and S. kurtzianum. The majority (90 %) of A. solani isolates carried the mating type locus 1 (MAT1) idiomorph MAT1-1 while the majority (88 %) of A. tomatophila isolates carried the MAT1-2 idiomorph.

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Acknowledgments

The authors are grateful to Dr. E. G. Simmons (Crawfordsville, IN, USA) for providing representative Alternaria cultures, to Dr. T. L. Peever (Pullman, WA, USA) for editing and thoughtful commenting on the draft of this manuscript and to Dr. I. A. Kazartsev and N. S. Pilshchikova for help with the DNA sequencing. This work was supported by the Russian Foundation for Basic Research (grant # 12-04-00677). Equipment of the Centre of Collective Use “Innovative Technologies of Plant Protection” was used.

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  1. All-Russian Institute of Plant Protection, Shosse Podbelskogo 3, Saint Petersburg, 196608, Russia

    Philipp B. Gannibal, Alexandra S. Orina, Nina V. Mironenko & Mark M. Levitin

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  1. Philipp B. Gannibal
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  2. Alexandra S. Orina
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  3. Nina V. Mironenko
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Correspondence to Philipp B. Gannibal.

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Gannibal, P.B., Orina, A.S., Mironenko, N.V. et al. Differentiation of the closely related species, Alternaria solani and A. tomatophila, by molecular and morphological features and aggressiveness. Eur J Plant Pathol 139, 609–623 (2014). https://doi.org/10.1007/s10658-014-0417-6

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