Abstract
Knowledge about the mechanism of transmission of systemic pathogens of citrus species is highly important for the safe movement of citrus germplasm, management of citrus mother trees, and also production of young plants. Among systemic pathogens of citrus, Xylella fastidiosa the causal agent of the citrus variegated chlorosis (CVC), is one the most important pathogens causing decline in tree vigour and productivity. Seven-year experiments were conducted to evaluate the hypothesis of seed-to-seedling transmission of X. fastidiosa. This bacterium was found colonizing the fruit (exocarp, central axis and mesocarp) and the seed parts (seed coat and endosperm plus embryo). After 7 years of PCR assay, no positive PCR detection of X. fastidiosa was confirmed in seedlings propagated from the seeds infected with X. fastidiosa. This result demonstrates the lack of seed-to-seedling transmission of this bacterium.
References
Agarwal, V. K., & Sinclair, J. B. (1987). Principles of seed pathology (Vol. 1). Boca Raton: CRC Press.
Albrecht, U., & Bowman, K. D. (2009). Candidatus Liberibacter asiaticus and Huanglongbing effects on citrus seeds and seedlings. HortScience, 44, 1967–1973.
Almeida, R. P. P., Coletta-Filho, H. D., & Lopes, J. R. S. (2013). Xylella fastidiosa–Citrus variegated chlorosis. In D. Liu (Ed.), Manual of security sensitive microbes and toxins. Taylor & Francis Group, USA. (in press).
Bridges, G. D., Youtsey, C. O., & Nixon, R. R. (1965). Observation indicating psrosis transmission by seed of Carrizo citrange. Proccedings of the Florida State Horticultural Society, 78, 48–50.
Camara, R. C., Vigo, S. C., & Maringoni, A. C. (2009). Plant-to-seed transmission of Curtobacterium flaccumfaciens pv. flaccumaciens in a dry bean cultivar. Journal of Plant Pathology, 91, 549–554.
Carnpiglia, H. G., Silveira, C. M., & Salibe, A. A. (1976). Psorosis transmission through seeds of trifoliate orange. Proceedings of the 7th Conference of International Organization of Citrus Virologists, 132–134. Riverside, CA.
D’Onghia, A. M., Djelouah, K., & Savino, V. (2000). Serological detection of Citrus psorosis virus in seeds but not in seedlings of infected mandarin and sour orange. Journal of Plant Pathology, 82, 233–235.
Garcia, A. L., Torres, S. C. Z., Heredia, M., & Lopes, S. A. (2012). Citrus responses to Xylella fastidiosa infection. Plant Disease, 96, 1245–1249.
Gitaitis, R., & Walcott, R. (2007). The epidemiology and management of seedborne bacterial diseases. Annual Review of Phytopathology, 45, 371–397.
Graham, J. H., Johnson, E. G., Bright, D. B., & Irey, M. S. (2011). Lack of development of Huanglongbing in seedlings from citrus seed. Proccedings of the Florida State Horticultural Society, 124, 79–84.
Hartung, J. S., Halbert, S. E., Pelz-Stelinski, K., Brlansky, R. H., Chen, C., & Gmitter, F. G. (2010). Lack of evidence for transmission of ‘Candidatus Liberibacter asiaticus’ through citrus seed taken from affected fruit. Plant Disease, 94, 1200–1205.
Hilf, M. E. (2011). Colonization of citrus seed coats by ‘Candidatus Liberibacter asiaticus’: implications for seed transmission of the bacterium. Phytopathology, 101, 1242–1250.
Laranjeira, F. F., Bergamin, F. A., & Amorim, L. (1998). Dynamics and structure of citrus variegated chlorosis (CVC) foci. Fitopatologia Brasileira, 23, 36–41.
Laranjeira, F. F., Pompeu, J., Jr., & Palazzo, D. (2000). Sementes de frutos de laranjeira ‘Natal’ afetados pela clorose variegada dos citros: germinação, crescimento de plântulas e não transmissão de Xylella fastidiosa. Laranja, 1, 161–173 (in Portuguese).
Li, W. B., Pria Júnior, W. D., Lacava, P. M., Qin, X., & Hartung, J. S. (2003). Presence of Xylella fastidiosa in sweet orange fruit and seeds and its transmission to seedlings. Phytopathology, 93, 953–958.
Minsavage, G. V., Thompson, C. M., Hopkins, D. L., Leite, R. M. V. B. C., & Stall, R. E. (1994). Development of a polymerase chain-reaction protocol for detection of Xylella fastidiosa in plant tissue. Phytopathology, 84, 456–461.
Murray, M. G., & Thompson, W. F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8, 4321–4325.
Roberts, S. J., Hiltunen, L. H., Hunter, P. J., & Brough, J. (1999). Transmission from seed to seedlings and secondary spread of Xanthomonas campestris pv. campestris in Brassica transplants: effects of dose and watering regime. European Journal of Plant Pathology, 105, 879–889.
Roistacher, C. N. (1993). Psorosis—A review. Proceedings of the 12th Conference of International Organization of Citrus Virologists, 139–154. Riverside, CA.
Shokrollah, H., Abdullah, T. L., Sijam, K., & Abdullah, S. N. A. (2009). Determination of the presence of huanglongbing in seeds and movement of the pathogen in Citrus reticulata. American Journal of Applied Sciences, 6, 1180–1185.
Torres, J. P., & Maringoni, A. C. (1997). Reaction of dry bean to Xanthomonas campestris pv. phaseoli and seed transmission of the bacterium. Fitopatologia Brasileira, 22, 546–549.
Xu, X., Miller, S. A., Baysal-Gurel, F., Gartemann, K.-H., Eichenlaub, R., & Rajashekara, G. (2010). Bioluminescence imaging of Clavibacter michiganensis subsp. michiganensis infection of tomato seeds and plants. Applied and Environmental Microbiology, 76, 3978–3988.
Yamamoto, P. T., Felippe, M. R., Caetano, A. C., Sanches, A. L., & Lopes, J. R. S. (2007). First report of Fingeriana dubia Cavichioli transmitting Xylella fastidiosa to citrus. Fitopatologia Brasileira, 32, 266.
Acknowledgments
We thank our lab colleagues and reviewers for constructive suggestions and discussions. HDCF and MAC thanks to CNPq for the scholarship support.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Coletta-Filho, H.D., Carvalho, S.A., Silva, L.F.C. et al. Seven years of negative detection results confirm that Xylella fastidiosa, the causal agent of CVC, is not transmitted from seeds to seedlings. Eur J Plant Pathol 139, 593–596 (2014). https://doi.org/10.1007/s10658-014-0415-8
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10658-014-0415-8