Abstract
Isolates of R. solani AG 2–1, AG 8, AG 10 and binucleate Rhizoctonia (Ceratobasidium spp.) were tested for virulence on Brassica crops in growth chamber experiments. Isolate virulence and genotype resistance were determined based on percent of seedling survival, shoot length reduction, and shoot fresh weight. Isolates had significant effects on all tested measurements, compared to the non-inoculated controls. Rhizoctonia solani AG 2–1 appears to be the most aggressive pathogen on all tested genotypes followed by R. solani AG 8, binucleate Rhizoctonia and R. solani AG 10, respectively. Genotype by isolate interaction effects were found to be significant for percent of seedling survival and shoot length reduction. None of the tested genotypes exhibited any level of resistance to R. solani AG 2–1, but three promising genotypes with moderate levels of resistance to R. solani AG 10, R. solani AG 8 and binucleate Rhizoctonia were identified. Moderate heritability (0.57) was observed for the percent of seedling survival in the resistant genotype KS4022.
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References
Acharya, S. N., Verma, P. R., Dueck, J., & Downey, R. K. (1984). Screening rapeseed/canola for resistance to damping-off and seedling root rot caused by Rhizoctonia solani. Canadian Journal of Plant Pathology, 6, 325–328.
Burton, G. W. (1951). Quantitative inheritance in earl millet (Pennisetum glaucum). Agronomy Journal, 43, 409–417.
Gill, J. S., Sivasithamparam, K., & Smettem, K. R. J. (2000). Soil types with different texture affects development of Rhizoctonia root rot of wheat seedlings. Plant and Soil, 221, 113–120.
Hang, A. N. (1996). Canola as a rotational crop in Washington cropping system. Pacific Northwest Canola Conference Proceedings. Nov. 10–12. Great Falls, MT.
Khangura, R., Barbetti, M. J., & Sweetingham, M. W. (1999). Characterization and pathogenicity of Rhizoctonia species on canola. Plant Disease, 83, 714–721.
Lamprecht, S. C., Tewoldemedhin, Y. T., Calitz, F. J., & Mazzola, M. (2011). Evaluation of strategies for the control of canola and lupin seedling diseases caused by Rhizoctonia anastomosis groups. European Journal of Plant Pathology, 130, 427–439.
MacNish, G. C., Carling, D. E., Sweetingham, M. W., Ogoshi, A., & Brainard, K. A. (1995). Characterisation of anastamosis group 10 (AG-10) of Rhizoctonia solani. Australian Plant Pathology, 24, 252–260.
Mazzola, M., & Gu, Y. (2002). Wheat genotype-specific induction of soil microbial communities suppressive to disease incited by Rhizoctonia solani anastomosis group (AG)-5 and AG 8. Phytopathology, 92, 1300–1307.
Okubara, P. A., Schroeder, K. L., & Paulitz, T. C. (2008). Identification and quantification of pathogenic Rhizoctonia solani and R. oryzae using real-time PCR. Phytopathology, 98, 837–847.
Paulitz, T. C., & Okubara, P. A. (2006). First report of damping-off of canola caused by Rhizoctonia solani AG 2–1 in Washington State. Plant Disease, 90, 829.
Paulitz, T. C., & Schroeder, K. L. (2005). A new method for the quantification of Rhizoctonia solani and Rhizoctonia oryzae from soil. Plant Disease, 89, 767–772.
Paulitz, T. C., Smiley, R., & Cook, R. J. (2002). Insights into the prevalence and management of soilborne cereal pathogens under direct seeding in the Pacific Northwest, USA. Canadian Journal of Plant Pathology, 24, 416–428.
Paulitz, T. C., Smith, J. D., & Kidwell, K. K. (2003). Virulence of Rhizoctonia oryzae on wheat and barley cultivars from the Pacific Northwest. Plant Disease, 87, 51–55.
Schroeder, K. L., & Paulitz, T. C. (2008). Effect of inoculum density and soil tillage on the development and severity of Rhizoctonia root rot. Phytopathology, 98, 304–314.
Schroeder, K. L., and Paulitz, T. C. (2012a). First report of a Ceratobasidium sp. causing root rot on canola in Washington State. Plant Disease, 96, 591.
Schroeder, K. L., & Paulitz, T. C. (2012b). First report of root rot caused by Rhizoctonia solani AG-10 on canola in Washington State. Plant Disease, 96, 584.
Smiley, R. W., & Uddin, W. (1993). Influence of soil temperature on Rhizoctonia root rot (R. solani AG 8 and R. oryzae) of winter wheat. Phytopathology, 83, 777–785.
Smiley, R. W., Ogg, A. G., Jr., & Cook, R. J. (1992). Influence of glyphosate on Rhizoctonia root rot, growth, and yield of barley. Plant Disease, 76, 937–942.
Wicks, T., Walker, G., Pederick, S., & Anstis, S. (2010). Onion stunting in South Australia associated with Rhizoctonia solani AG 8. Australasian Plant Pathology, 40, 126–132.
Yang, J., & Verma, P. R. (1992). Screening genotypes for resistance to preemergence damping-off and postemergence seedling root rot of oilseed rape and canola caused by Rhizoctonia AG2-1. Crop Protection, 11, 443–448.
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We thank the Biofuels Cropping Systems Research and Extension Project of Washington State and the Washington and Idaho Canola Commission for financial support.
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Babiker, E.M., Hulbert, S.H., Schroeder, K.L. et al. Evaluation of Brassica species for resistance to Rhizoctonia solani and binucleate Rhizoctonia (Ceratobasidum spp.) under controlled environment conditions. Eur J Plant Pathol 136, 763–772 (2013). https://doi.org/10.1007/s10658-013-0205-8
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DOI: https://doi.org/10.1007/s10658-013-0205-8