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Genetic diversity of ‘Cadidatus Liberibacter solanacearum’ strains in the United States and Mexico revealed by simple sequence repeat markers

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Abstract

‘Candidatus Liberibacter solanacearum’ is associated with the Zebra Chip (ZC) disorder of potatoes. A panel of eight simple sequence repeat (SSR) markers was developed and used to genetically characterize ‘Ca. L. solanacearum’ strains obtained from ZC-affected potato plants in the United States and Mexico. The multilocus SSR markers in this study effectively differentiated genotypes and estimated genetic diversity of ‘Ca. L. solanacearum’ strains. Genotype assignment analyses identified two major lineages of ‘Ca. L. solanacearum’ in the North American populations while only one lineage type was identified in Mexican population. No clear genetic structure was found among haplotypes based on geographical proximity or host. The high resolution power of the SSR marker system developed in this study provides a useful tool for genotyping closely related strains and tracking sources of the pathogen. Genotype information combined with epidemiological data will advance knowledge of ZC disease and will facilitate development of effective disease management.

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Acknowledgements

This work was supported by 2009 USDA National Institute of Food and Agriculture (NIFA) Specialty Crops Research Initiative (SCRI). Trade names or commercial products in this publication are mentioned solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture.

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Authors and Affiliations

  1. Crop Diseases, Pests and Genetics research Unit, USDA-ARS, 9611 S. Riverbend Avenue, Parlier, CA, 93648, USA

    Hong Lin, Md Sajedul Islam, Yang Bai & Suque Lan

  2. Guangxi Citrus Research Institute, Guilin, China

    Yang Bai

  3. Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108, USA

    Amin Wen & Neil C. Gudmestad

  4. Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, China

    Suque Lan

  5. USDA-ARS-Crop Diseases, Pests and Genetics Research Unit, Parlier, California, CA, 93648, USA

    Hong Lin

  6. USDA-ARS, 9611 S. Riverbend Avenue, Parlier, CA, 93648, USA

    Edwin L. Civerolo

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  1. Hong Lin
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  2. Md Sajedul Islam
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  3. Yang Bai
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  4. Amin Wen
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  5. Suque Lan
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  6. Neil C. Gudmestad
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  7. Edwin L. Civerolo
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Correspondence to Hong Lin.

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Lin, H., Islam, M.S., Bai, Y. et al. Genetic diversity of ‘Cadidatus Liberibacter solanacearum’ strains in the United States and Mexico revealed by simple sequence repeat markers. Eur J Plant Pathol 132, 297–308 (2012). https://doi.org/10.1007/s10658-011-9874-3

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