Abstract
Three genetic variants of Grapevine leafroll-associated virus 3 (GLRaV-3) were identified from vineyards of the Western Cape, South Africa. In a previous study, three full genome sequences of isolates representing each of the variant groups were determined. The three variant groups were represented by accessions 621, 623 and PL-20, of variant groups I, II and III respectively. A specific single strand conformation polymorphism (SSCP) profile was assigned to each variant which was used as a quick, reliable detection and differentiation method. In this study we analysed the occurrence of these three GLRaV-3 variants in mother blocks in different cultivars and from different vine growing regions using SSCP. The majority of the plants studied, were infected with the group II variant, similar to isolates 623 and GP18. The distribution of three GLRaV-3 variants within a spatio-temporally recorded cluster of diseased plants was studied by means of SSCP profile analysis of ORF5 amplified PCR products. We showed that different GLRaV-3 variants are transmitted to adjacent plants in an infection cluster. Results showed that, in some leafroll disease clusters, the variant that was present in the original GLRaV-3 infected plant of a cluster was transmitted to adjacent plants in a row and across rows.
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Acknowledgements
The research in this study was funded by the South African industry partner, Winetech, in collaboration with the National Research Foundation’s THRIP programme and ARC-PPRI. We thank Elsa van Niekerk, ARC-PPRI, for her help with some graphics presented in the paper.
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Jooste, A.E.C., Pietersen, G. & Burger, J.T. Distribution of grapevine leafroll associated virus-3 variants in South African vineyards. Eur J Plant Pathol 131, 371–381 (2011). https://doi.org/10.1007/s10658-011-9814-2
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DOI: https://doi.org/10.1007/s10658-011-9814-2