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Metabolomics technology to phenotype resistance in barley against Gibberella zeae

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Abstract

The mechanisms of resistance in barley to fusarium head blight (FHB), caused by Gibberella zeae are complex. Metabolomics technology was explored to phenotype resistance. Spikelets of barley genotypes with contrasting levels of resistance to FHB, mock inoculated or with the pathogen, were extracted with aqueous methanol and the metabolites were analyzed using liquid chromatography and hybrid mass spectrometry. Peaks were de-convoluted using XCMS and annotated using CAMERA and IntelliXtract bioinformatics tools. A t-test, of a total of 1608 purified peaks, selected 626 metabolites with significant treatment effects, of which 161 were identified as resistance related (RR) metabolites. A total of 53 metabolites, that are RR or pathogenicity related (PR), were assigned with putative compound names. These mainly belonged to three metabolic pathways: fatty acid (jasmonic acid, methyl jasmonate, 9,10- dihydro-isojasmonate, linolenic acid, linoleic acid, traumatic acid), phenylpropanoid (p-coumaric acid, caffeyl alcohol, dimethoxy-4-phenylcoumarin, rosmarinic acid, diphyllin, 5-methoxypodophyllotoxin) and flavonoid (naringenin, catechin, quercetin, and alpinumisoflavone). A few PR/RR metabolites significantly reduced mycelial growth of G. zeae in vitro.

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Acknowledgements

This project was funded by the Ministère de l’Agriculture, des Pêcheries et de l’Alimentation du Québec (MAPAQ), Centre de recherche sur les grains inc. (CEROM), and the Fédération des producteurs de porc du Québec (FPPQ), Québec, Canada.

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Authors and Affiliations

  1. Plant Science Department, McGill University, Sainte-Anne-de-Bellevue, QC, H9X 3V9, Canada

    G. Kenchappa Kumaraswamy, Venkatesh Bollina & Ajjamada C. Kushalappa

  2. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, 960 Carling Ave., Ottawa, ON, K1A 0C6, Canada

    Thin M. Choo

  3. Centre de recherche sur les grains inc., 740 chemin Trudeau, Saint-Mathieu-de-Beloeil, QC, J3G 4S5, Canada

    Yves Dion

  4. Centre de recherche sur les grains inc., 2700 rue Einstein, Ste. Foy, QC, G1P 3W8, Canada

    Sylvie Rioux

  5. Goodman Cancer Research Centre, McGill University, 1160 Pine Ave West, Montréal, QC, H3A 1A3, Canada

    Orval Mamer

  6. Institut de recherches cliniques de Montréal, 110 av. des Pins Ouest, Montréal, QC, H2W 1R7, Canada

    Denis Faubert

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  1. G. Kenchappa Kumaraswamy
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Correspondence to Ajjamada C. Kushalappa.

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Kumaraswamy, G.K., Bollina, V., Kushalappa, A.C. et al. Metabolomics technology to phenotype resistance in barley against Gibberella zeae . Eur J Plant Pathol 130, 29–43 (2011). https://doi.org/10.1007/s10658-010-9729-3

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