Abstract
Plots in two vineyards in the Golan Heights, Israel were treated with six botryticides during three growing seasons with 3 applications per season. Applications of fenhexamid, pyrimethanil and cyprodinil + fludioxonil were effective, resulting in 52–65% and 53–63% mean reduction in grey mould incidence and severity, respectively. Carbendazim, fluazinam and iprodione were ineffective or slightly effective. Five hundred and sixteen B. cinerea isolates were collected from infected berries or trapped from the air in the vineyards, and profiles of sensitivity to benomyl, fenhexamid, fluazinam, fludioxonil, iprodione and pyrimethanil were established for each of the isolates based on a mycelial growth test. Seventy-four percent of the isolates were sensitive to the six tested fungicides, and the other 26% of the isolates were classified into 10 phenotypes characterized by resistance to one or more fungicides. Resistant isolates showed fitness parameters similar or reduced in comparison to sensitive isolates. Resistance to benzimidazoles and to dicarboximides was the most frequent (up to 25%) and apparently pre-existed in the populations tested. Increased frequency of benzimidazole resistance, but not dicarboximide resistance, was observed following the 3 years of applications of the fungicides. High level resistance to pyrimethanil was present at a frequency of about 2% in both vineyards in the first 2 years of the sampling survey and reached 10% in the third year at Site 2. A few isolates were resistant to fenhexamid or fludioxonil (0.8 or 0.2%, respectively). No strong resistance to fluazinam was detected, although numerous, less sensitive isolates, presumably possessing multi-drug resistance traits, were recovered at higher frequency from the plots treated with fluazinam than from the untreated plots.
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Bardas, G. A., Myresiotis, C. K., & Karaoglandis, G. S. (2008). Stability and fitness of anilinopyrimidine-resistant strains of Botrytis cinerea. Phytopathology, 98, 443–450.
Baroffio, C. A., Siegfried, W., & Hilber, U. W. (2003). Long-term monitoring for resistance of Botryotinia fuckeliana to anilinopyrimidine, phenylpyrrole, and hydroxyanilide fungicides in Switzerland. Plant Disease, 87, 662–666.
Beever, R. E., Laracy, E. P., & Pak, H. A. (1989). Strains of Botrytis cinerea resistant to dicarboximide and benzimidazole fungicides in New Zealand vineyards. Plant Patholology, 38, 427–437.
Campbell, C. L., & Madden, L. V. (1990). Introduction to plant disease epidemiology. New York: Wiley.
Chapeland, F., Fritz, R., Lanen, C., Gredt, M., & Lerouz, P. (1999). Inheritance and mechanisms of resistance to anilinopyrimidine fungicides in Botrytis cinerea (Botryotinia fuckeliana). Pesticide Biochemistry and Physiology, 64, 85–100.
Edwards, S. G., & Seddon, B. (2001). Selective media for the specific isolation and enumeration of Botrytis cinerea conidia. Letters in Applied Microbiology, 32, 63–66.
Elad, Y., Yunis, H., & Katan, T. (1992). Multiple resistance to benzimidazoles, dicarboximides and diethophencarb in field isolates of Botrytis cinerea in Israel. Plant Pathology, 41, 41–46.
Elmer, P., & Michailides, T. (2004). Epidemiology of Botrytis cinerea in orchard and vine crops. In Y. Elad, P. Williamson, P. Tudzinski, & N. Delen (Eds.), Botrytis: Biology, pathology and control (pp. 243–272). Dordrecht: Kluwer Academic.
Esterio, M., Auger, J., & Garcia, H. (2007). First report of fenhexamid resistant isolates of Botrytis cinerea on grapevine in Chile. (Abstr.). Plant Disease, 91, 768.
Fillinger, S., Leroux, P., Auclair, C., Barreau, C., Al Hajj, C., & Debieu, D. (2008). Genetic analysis of fenhexamid-resistant field isolates of the phytopathogenic fungus Botrytis cinerea. Antimicrobial Agents and Chemotherapy, 52, 3933–3940.
Fitt, B. D. L., Creighton, N. F., & Brainbridge, A. (1985). Role of wind and rain in dispersal of Botrytis fabae conidia. Transactions British Mycological Society, 85, 307–312.
Förster, H., Driever, G. F., Thompson, D. C., & Adaskaveg, J. E. (2007). Postharvest decay management for stone fruit crops in California using the “reduced-risk” fungicides fludioxonil and fenhexamid. Plant Disease, 91, 209–215.
Förster, B., & Staub, T. (1996). Basis for use strategies of anilinopyrimidine and phenylpyrrole fungicides against Botrytis cinerea. Crop Protection, 15, 529–537.
Hänßler, G., & Pontzen, R. (1999). Effect of fenhexamid on the development of Botrytis cinerea. Pflanzenschutz-Nachrichten Bayer, 52, 158–176.
Hilber, U. W., & Hilber-Bodmer, M. (1998). Genetic basis and monitoring of resistance of Botryotinia fuckeliana to anilinopyrimidines. Plant Disease, 82, 496–500.
Hilber, U. W., Schwinn, F. J., & Schuepp, H. (1995). Comparative resistance patterns of fludioxonil and vinclozolin in Botryotinia fuckeliana. Journal of Phytopathology, 143, 423–428.
Jo, Y.-K., Niver, A. L., Rimelspach, J. W., & Boehm, M. J. (2006). Fungicide sensitivity of Sclerotinia homoeocarpa from golf courses in Ohio. Plant Disease, 90, 807–813.
Kalamarakis, A. E., Petsikos-Panagiotarou, N., & Ziogas, B. N. (2000). Activity of fluazinam against strains of Botrytis cinerea resistant to benzimidazoles and/or dicarboximides and to a benzimidazole-phenylcarbamate mixture. Journal of Phytopathology, 148, 449–455.
Köller, W., Wilcox, W., Barnard, J., & Braun, P. (1997). Detection and quantification of resistance of Venturia inaequalis populations to sterol demethylation inhibitors. Phytopathology, 87, 184–190.
Korolev, N., Mamiev, M., & Elad, Y. (2009). Resistance to fungicides among Botrytis cinerea isolates from tomato and other hosts in Israel. Acta Horticulturae, 808, 367–375.
Kretschmer, M., Leroch, M., Mosbach, A., Walker, A.-S., Fillinger, S., Mernke, D., et al. (2009). Fungicide-driven evolution and molecular basis of multidrug resistance in field populations of the grey mould fungus Botrytis cinerea. PLoS Pathogens, 5, Article e1000696. Retrieved June 15, 2010, from http://www.plospathogens.org
Leroux, P. (2004). Chemical control of Botrytis and its resistance to chemical fungicides. In Y. Elad, P. Williamson, P. Tudzinski, & N. Delen (Eds.), Botrytis: Biology, pathology and control (pp. 195–222). Dordrecht: Kluwer Academic.
Leroux, P., Chapeland, F., Desbrosses, D., & Gredt, M. (1999). Patterns of cross-resistance to fungicides in Botryotinia fuckeliana (Botrytis cinerea) isolates from French vineyards. Crop Protection, 18, 687–697.
Löchner, F. J., Lorenz, G., & Beetz, K.-J. (1987). Resistance management strategies for dicarboximide fungicides in grapes: results of six years’ trial work. Crop Protection, 6, 139–147.
Ma, Z., & Michailides, T. J. (2005). Genetic structure of Botrytis cinerea populations from different host plants in California. Plant Disease, 89, 1083–1089.
Morton, V., & Staub, T. (2008). A short history of fungicides. APSnet Feature, March 2008. Retrieved from http://apsnet.org/online/feature/fungi
Myresiotis, C. K., Karaoglandis, G. S., & Tzavella-Klonari, K. (2007). Resistance of Botrytis cinerea isolates from vegetable crops to anilidopyrimidine, phenylpyrrole, hydroxyanilide, benzimidazole, and dicarboximide fungicides. Plant Disease, 91, 407–413.
Oshima, M., Fujimura, M., Banno, S., Hashimoto, C., Motoyama, T., Ichiishi, A., et al. (2002). A point mutation in the two-component histidine kinase BcOS-1 gene confers dicarboximide resistance in field isolates of Botrytis cinerea. Phytopathology, 92, 75–80.
Raposo, R., Delcan, J., Gomez, V., & Melgarejo, P. (1996). Distribution and fitness of isolates of Botrytis cinerea with multiple fungicide resistance in Spanish greenhouses. Plant Pathology, 45, 497–505.
Raposo, R., Gomez, V., Urrutia, T., & Melgarejo, P. (2000). Fitness of Botrytis cinerea associated with dicarboximide resistance. Phytopathology, 90, 1246–1249.
Savary, S., Delbac, L., Rochas, A., Taisant, G., & Willocquet, L. (2009). Analysis of nonlinear relationships in dual epidemics, and its application to the management of grapevine downy and powdery mildews. Phytopathology, 99, 930–942.
Suty, A., Pontzen, R., & Stenzel, K. (1999). Fenhexamid—sensitivity of Botrytis cinerea: determination of baseline sensitivity and assessment of the risk of resistance. Pflanzenschutz-Nachrichten Bayer, 52, 145–157.
Vignutelli, A., Hilber-Bodmer, M., & Hilber, U. W. (2002). Genetic analysis of resistance to the phenylpyrrole fludioxonil and the dicarboximide vinclozolin in Botryotinia fuckeliana (Botrytis cinerea). Mycological Research, 106, 329–335.
Yourman, L. F., & Jeffers, S. N. (1999). Resistance to benzimidazole and dicarboximide fungicides in greenhouse isolates of Botrytis cinerea. Plant Disease, 83, 569–575.
Yourman, L. F., Jeffers, S. N., & Dean, R. A. (2001). Phenotype instability in Botrytis cinerea in the absence of benzimidazole and dicarboximide fungicides. Phytopathology, 91, 307–315.
Ziogas, B. N., Markoglou, A. N., & Spyropoulou, V. (2005). Effect of phenylpyrrole-resistance mutations on ecological fitness of Botrytis cinerea and their genetic basis in Ustilago maydis. European Journal of Plant Pathology, 113, 83–100.
Acknowledments
This research was partially supported by a fellowship from the Israeli Ministry of Immigration and by the research fund of the Chief Scientist of the Israeli Ministry of Agriculture and Rural Development, project no. 132-1106-03. The authors acknowledge Ruth Harpaz for her assistance with the disease evaluations and thank Suleiman Farhat for spraying the experiments. Contribution No 521/09 from the ARO, Volcani Center, Institute of Plant Protection.
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Korolev, N., Mamiev, M., Zahavi, T. et al. Screening of Botrytis cinerea isolates from vineyards in Israel for resistance to fungicides. Eur J Plant Pathol 129, 591–608 (2011). https://doi.org/10.1007/s10658-010-9723-9
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DOI: https://doi.org/10.1007/s10658-010-9723-9