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Microsatellite based population structure of Plasmopara viticola at single vine scale

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Abstract

The genetic structure of a Plasmopara viticola population was characterized on five single vines, one for each cultivar Regent, Merlot, Isabella, Müller-Thurgau and Solaris, using four neutral specific polymorphic microsatellite markers. Five-hundred and seventy samples were collected at four dates in the period between the 10th of July and the 23rd of August 2006. On average over all five cultivars, 67% of the genotypes present on the single selected vines derived from primary infections and caused 37% of the lesions genotyped. Fifty-three percent of these genotypes occurred only once on the vine throughout the survey period, while 14% were able to asexually reproduce on the selected single vine throughout the survey period, causing 23% of the lesions. Thirty-three percent of the genotypes on the single vine derived from other vines, 28% from vines of other cultivars in the other rows, and 5% from vines of the same cultivar in the same row. New primary infections appear all along the sampling dates. The overwhelmingly quantitative role of primary infections at vineyard scale was known, however here we observed the phenomenon also at the single vine scale and the reduced contribution of secondary lesions to the populations present on more resistant cultivars compared to the susceptible cultivars. As the sampling extended almost to defoliation, the results are judged to be representative of a typical P. viticola epidemic.

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References

  • Aziz, A., Poinssot, B., Daire, X., Adrian, M., Bézier, A., Lambert, B., et al. (2003). Laminarin elicits defence responses in grapevine and induces protection against Botrytis cinerea and Plasmopara viticola. Molecular Plant-Microbe Interactions, 16, 1118–1128.

    Article  CAS  PubMed  Google Scholar 

  • Bellin, D., Peressotti, E., Merdinoglu, D., Wiedemann-Merdinoglu, S., Adam-Blondon, A.-F., Cipriani, G., et al. (2009). Resistance to Plasmopara viticola in grapevine ‘Bianca’ is controlled by a major dominant gene causing localised necrosis at the infection site. Theoretical and Applied Genetics, 120(1), 163–176.

    Article  PubMed  Google Scholar 

  • Beresford, R. M., Wood, P. N., Shaw, P. W., & Taylor, T. J. (2008). Application of fungicides during leaf fall to control apple scab (Venturia inaequalis) in the following season. New Zealand Plant Protection, 61, 59–64.

    CAS  Google Scholar 

  • Boso, S., & Kassemeyer, H.-H. (2008). Different susceptibility of European grapevine cultivars for downy mildew. Vitis, 47(1), 39–49.

    Google Scholar 

  • Calderon, A. A., Zapata, J. M., & Ros Barcelo, A. (1994). Peroxidase-mediated formation of resveratrol oxidation products during the hypersensitive-like reaction of grapevine cells to an elicitor from Trichoderma viride. Physiological and Molecular Plant Pathology, 44, 289–299.

    Article  CAS  Google Scholar 

  • Campbell, C. L., & Madden, L. V. (1990). Introduction to plant disease epidemiology. New York: Wiley.

    Google Scholar 

  • Dai, G. H., Andary, C., Mondolot-Cosson, L., & Boubals, D. (1995a). Histochemical studies on the interaction between three species of grapevine, Vitis vinfera, V. rupestris and V. rotundifolia and the downy mildew fungus, Plasmopara viticola. Physiological and Molecular Plant Pathology, 46, 177–188.

    Article  Google Scholar 

  • Dai, G. H., Andary, C., Mondolot-Cosson, L., & Boubals, D. (1995b). Involvement of phenolic compounds in the resistance of grapevine callus to downy mildew (Plasmopara viticola). European Journal of Plant Pathology, 101, 541–547.

    Article  CAS  Google Scholar 

  • Díez-Navajas, A. M., Wiedemann-Merdinoglu, S., Greif, C., & Merdinoglu, D. (2008). Nonhost versus host resistance to the grapevine downy mildew, Plasmopara viticola, studied at the tissue level. Phytopathology, 98, 776–780.

    Article  PubMed  Google Scholar 

  • Eugster, C. (2003). Relative contribution of oosporic and sporangial inoculum to epidemics of grapevine downy mildew and genetic structure of Plasmopara viticola in a New York vineyard assessed by microsatellite technique. Diploma Thesis, ETH-Zürich.

  • Ficke, A., Gadoury, D. M., Seem, R. C., Godfrey, D., & Dry, I. B. (2004). Host barriers and responses to Uncinula necator in developing grape berries. Phytpathology, 94, 438–445.

    Article  Google Scholar 

  • Gessler, C., Pertot, I., & Gobbin, D. (2006). Genetic structure and epidemiology of Plasmopara viticola populations. In I. Pertot, C. Gessler, D. Gadoury, W. Gubler, H.-H. Kassemeyer & P. Magarey (Eds.), Proceedings of the 5th International workshop on grapevine downy and powdery mildew (pp. 75–77).

  • Gindro, K., Pezet, R., & Viret, O. (2003). Histological study of the responses of two Vitis vinifera cultivars (resistant and susceptible) to Plasmopara viticola infections. Plant Physiology and Biochemistry, 41, 846–853.

    Article  CAS  Google Scholar 

  • Gobbin, D., Pertot, I., & Gessler, C. (2003a). Identification of microsatellite markers for Plasmopara viticola and establishment of high throughput method for SSR analysis. European Journal of Plant Pathology, 109, 153–164.

    Article  CAS  Google Scholar 

  • Gobbin, D., Pertot, I., & Gessler, C. (2003b). Genetic structure of a Plasmopara viticola population in an isolated Italian mountain vineyard. Journal of Phytopathology, 151, 636–646.

    Article  CAS  Google Scholar 

  • Gobbin, D., Jermini, M., & Gessler, C. (2003c). The genetic underpinning of the minimal fungicide strategy. Bulletin OILB/SROP, 26(8), 101–104.

    Google Scholar 

  • Gobbin, D., Jermini, M., Loskill, B., Pertot, I., Raynal, M., & Gessler, C. (2005). Importance of secondary inoculum of Plasmopara viticola to epidemics of grapevine downy mildew. Plant Pathology, 54, 522–534.

    Article  Google Scholar 

  • Gobbin, D., Rumbou, A., Linde, C., & Gessler, C. (2006). Population genetic structure of Plasmopara viticola after 125 years of colonization in European vineyards. Molecular Plant Pathology, 6, 519–531.

    Article  Google Scholar 

  • Godard, S., Slacanin, I., Viret, O., & Gindro, K. (2009). Induction of defence mechanisms in grapevine leaves by emodin- and anthraquinone-rich plant extracts and their conferred resistance to downy mildew. Plant Physiology and Biochemistry, 47(9), 827–837.

    Article  CAS  PubMed  Google Scholar 

  • Heintz, C., & Blaich, R. (1989). Structural characters of epidermal cell walls and resistance to powdery mildew of different grapevine cultivars. Vitis, 28, 153–160.

    Google Scholar 

  • Holb, I., Heine, B., & Jeger, M. (2006). Effects of integrated control measures on earthworms, leaf litter and Venturia inaequalis infection in two European apple orchards. Agriculture, Ecosystems & Environment, 114(2–4), 287–295.

    Article  Google Scholar 

  • Horsfall, J. G., & Barratt, R. W. (1945). An improved grading system for measuring plant disease. Phytopathology, 35, 655.

    Google Scholar 

  • Hug, F. (2005). Genetic structure and epidemiology of Plasmopara viticola populations from Australian grape growing regions. Diploma Thesis, ETH-Zürich.

  • Jermini, M., Blaise, P., & Gessler, C. (2003). Response of grapevine growth and yield quantity to the application of a minimal fungicide strategy for the control of the downy mildew (Plasmopara viticola). Bulletin OILB/SROP, 26(8), 25–29.

    Google Scholar 

  • Jermini, M., Christen, D., Strasser, R., & Gessler, C. (2006). Impact of four years application of the minimal fungicide strategy for downy mildew control on the plant recovery capacities of Vitis vinifera cv Merlot. In I. Pertot, C. Gessler, D. Gadoury, W. Gubler, H.-H. Kassemeyer & P. Magarey (Eds.), Proceedings of the 5th International workshop on grapevine downy and powdery mildew (pp. 176–177).

  • Kast, W. K., Stark-Urnau, M., Seidel, M., & Gemmrich, A. R. (2000). Inter-isolate variation of virulence of Plasmopara viticola on resistant vine varieties. Mitteilungen Klosterneuburg Rebe und Wein, Obstbau und Früchteverwertung, 50(1), 38–42.

    Google Scholar 

  • Kennelly, M. M., Gadoury, D. M., Wilcox, W. F., Magarey, P. A., & Seem, R. C. (2007). Primary infection, lesion productivity, and survival of sporangia in the grapevine downy mildew pathogen, Plasmopara viticola. Phytopathology, 97, 512–522.

    Article  PubMed  Google Scholar 

  • Koopman, T., Linde, C. C., Fourie, P. H., & McLeod, A. (2007). A Population genetic structure of Plasmopara viticola in the Western Cape Province of South Africa. Molecular Plant Pathology, 8(6), 723–736.

    Article  PubMed  Google Scholar 

  • Kortekamp, A. (2006). Expression analysis of defence-related genes in grapevine leaves after inoculation with a host and a non-host pathogen. Plant Physiology and Biochemistry, 44, 58–67.

    Article  CAS  PubMed  Google Scholar 

  • Kortekamp, A., & Zyprian, E. (1999). Leaf hairs as a basic protective barrier against downy mildew of grape. Journal of Phytopathology, 147, 453–459.

    Article  Google Scholar 

  • Kortekamp, A., & Zyprian, E. (2003). Characterization of Plasmopara-resistance in grapevine using in vitro plants. Journal of Plant Physiology, 160, 1393–1400.

    Article  CAS  PubMed  Google Scholar 

  • Kortekamp, A., Wind, R., & Zyprian, E. (1998). Investigation of the interaction of Plasmopara viticola with susceptible and resistant grapevine cultivars. Journal of Plant Diseases and Protection, 105, 475–488.

    Google Scholar 

  • Kortekamp, A., Wind, R., & Zyprian, E. (1999). The role of hairs on the wettability of grapevine (Vitis spp.) leaves. Vitis, 38, 101–105.

    Google Scholar 

  • MacHardy, W. E., Gadoury, D. M., & Gessler, C. (2001). Parasitic and biological fitness of Venturia inaequalis: relationship to disease management strategies. Plant Disease, 85(10), 1036–1051.

    Article  Google Scholar 

  • Pezet, R., Gindro, K., Viret, O., & Spring, J.-L. (2004). Glycosylation and oxidative dimerization of resveratrol are respectively associated to sensitivity and resistance of grapevine cultivars to downy mildew. Physiological and Molecular Plant Pathology, 65, 297–303.

    Article  CAS  Google Scholar 

  • Richter, H., Pezet, R., Viret, O., & Gindro, K. (2006). Characterization of 3 new partial stilbene synthase genes out of over 20 expressed in Vitis vinifera during the interaction with Plasmopara viticola. Physiological and Molecular Plant Pathology, 67, 248–260.

    Article  Google Scholar 

  • Rumbou, A., & Gessler, C. (2004). Genetic dissection of Plasmopara viticola population from a Greek vineyard in two consecutive years. European Journal of Plant Pathology, 4, 379–392.

    Article  Google Scholar 

  • Rumbou, A., & Gessler, C. (2006). Particular structure of Plasmopara viticola populations evolved under Greek island conditions. Phytopathology, 96, 501–509.

    Article  PubMed  Google Scholar 

  • VIVC, Vitis International Variety Catalogue. (2007). IRZ, Institut für Rebenzüchtung Geilweilerhof.

  • Wong, F. P., Burr, H. N., & Wilcox, W. F. (2001). Heterothallism in Plasmopara viticola. Plant Pathology, 50, 427–432.

    Article  Google Scholar 

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Acknowledgements

This work was supported by SBF 03.0485-1 (EU-Project 501542 REPCO). The authors gratefully acknowledge Rocchina Abbas-Pennella for helping with the laboratory work, Michele Gusberti and Natasha Rosselli for helping with the collection of samples and disease assessment, and Roberto Rigoni and Mirto Ferretti for the maintenance work in the vineyard.

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Authors and Affiliations

  1. Institute of Integrative Biology (IBZ), ETH Zürich, CH-8092, Zürich, Switzerland

    Caterina L. Matasci, Davide Gobbin & Cesare Gessler

  2. Agroscope ACW Changins-Wädenswil, Swiss Federal Agricultural Research Station Changins, Centro di Cadenazzo, CH-6594, Contone, Switzerland

    Mauro Jermini

Authors
  1. Caterina L. Matasci
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  2. Mauro Jermini
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  3. Davide Gobbin
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  4. Cesare Gessler
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Correspondence to Cesare Gessler.

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Matasci, C.L., Jermini, M., Gobbin, D. et al. Microsatellite based population structure of Plasmopara viticola at single vine scale. Eur J Plant Pathol 127, 501–508 (2010). https://doi.org/10.1007/s10658-010-9615-z

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