Abstract
We isolated eight polymorphic microsatellite markers for the basidiomycete Armillaria cepistipes and characterised them by analysing 50 isolates representing two geographically distinct populations from Switzerland and the Ukraine. The number of alleles per locus and population varied from one to eight, resulting in 43 alleles over the eight loci and two populations. In both populations, no significant linkage disequilibrium was observed between pairs of loci. Significant (P < 0.05) deviations from Hardy-Weinberg equilibrium were observed at one locus in the Swiss population and at three loci in the Ukrainian population. Of the eight loci developed for A. cepistipes, six were also polymorphic in A. gallica, four in A. ostoyae, two in A. mellea, and one in A. borealis. Beside the potential to be used for population genetic studies on A. cepistipes, these microsatellites thus represent additional molecular markers for three of the four annulated Armillaria species occurring in Europe.
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We thank R. Holderegger for critical comments on the manuscript and the Competence Center Environment and Sustainability (CCES) of the ETH domain for financial support (project GEDIHAP).
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Prospero, S., Jung, E., Tsykun, T. et al. Eight microsatellite markers for Armillaria cepistipes and their transferability to other Armillaria species. Eur J Plant Pathol 127, 165–170 (2010). https://doi.org/10.1007/s10658-010-9594-0
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DOI: https://doi.org/10.1007/s10658-010-9594-0