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Tobacco ringspot virus persists in the shoot apical meristem but not in the root apical meristem of infected tobacco

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Abstract

We analysed the kinetics of virus distribution in Tobacco ringspot virus (TRSV)-infected tobacco. Infected plants developed asymptomatic leaves above the symptomatic leaves that exhibited ringspots. We detected virus in the shoot apical meristem (SAM). Time-course immunohistochemical microscopy showed that TRSV continuously distributed in the centres of the SAM and in leaf primordia. In contrast, TRSV infection was transient in the root meristem. In western blot and RT-PCR analyses, TRSV infection occurred only within and around the ringspot tissues of the symptomatic leaf; however, TRSV was not or hardly detected in the asymptomatic leaves of inoculated tobacco plants. These results indicate that TRSV persists in the SAM, but that TRSV infection is transient in the root meristem and asymptomatic leaves.

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Fig. 1
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Abbreviations

CP:

coat protein

dpi:

days post-inoculation

PAGE:

polyacrylamide gel electrophoresis

PBS:

phosphate-buffered saline

RAM:

root apical meristem

RT-PCR:

reverse transcriptase-polymerase chain reaction

SAM:

shoot apical meristem

TRSV:

Tobacco ringspot virus

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Acknowledgement

We thank Dr. Y. Honda of the National Agricultural Research Centre for providing the anti-TRSV antiserum.

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Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, 599-8531, Japan

    Fang Dong, Tomofumi Mochizuki & Satoshi T. Ohki

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  1. Fang Dong
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  2. Tomofumi Mochizuki
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  3. Satoshi T. Ohki
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Correspondence to Tomofumi Mochizuki.

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Dong, F., Mochizuki, T. & Ohki, S.T. Tobacco ringspot virus persists in the shoot apical meristem but not in the root apical meristem of infected tobacco. Eur J Plant Pathol 126, 117–122 (2010). https://doi.org/10.1007/s10658-009-9518-z

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  • DOI: https://doi.org/10.1007/s10658-009-9518-z

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