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The activity of amino acids produced by Paenibacillus macerans and from commercial sources against the root-knot nematode Meloidogyne exigua

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Abstract

The rhizobacterium Paenibacillus macerans was grown in tryptic soy broth and after separating the cells by centrifugation the activity of fractions of the supernatant was tested against Meloidogyne exigua juveniles. From HPLC analyses and spectral data, the most active fractions were found to contain alanine, glutamic acid, glycine, histidine, threonine and valine, which were probably produced by bacterial hydrolysis of proteic nutrients. Amino acids from commercial sources were then assayed to confirm these results and to evaluate their potential for the control of nematodes. LC50 of 26 and 283 μg ml−1 were shown for the nematicide aldicarb and L-cysteine respectively when tested on M. exigua juveniles. At a concentration 38.4 times>LC50, the amino acid diminished the nematode population on coffee plants to values statistically equal to those obtained with aldicarb at a concentration 19.2 times>LC50.

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Acknowledgements

The authors are grateful to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Project number 372284/2006-0), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG, Project number EDT 573/05) and Coordenação de Aperfeiçoamento de Nível Superior (CAPES, Project number 0389056), for financial support and fellowships. The authors also express their sincere thanks to Prof. Eduardo M. Cilli for the HPLC analyses carried out at Araraquara Chemistry Institute-UNESP.

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Authors and Affiliations

  1. Departamento de Química, Universidade Federal de Lavras, C.P. 3037, CEP 37200-000, Lavras, MG, Brazil

    Denilson F. Oliveira, Hudson W. P. Carvalho, Alessandro S. Nunes & Helvécio M. S. Júnior

  2. Instituto de Química, Universidade Estadual Paulista Júlio de Mesquita Filho, CEP 14800-900, Araraquara, SP, Brazil

    Geraldo H. Silva & Alberto J. Cavalheiro

  3. Departamento de Fitopatologia, Universidade Federal de Lavras, C.P. 3037, CEP 37200-000, Lavras, MG, Brazil

    Vicente P. Campos

Authors
  1. Denilson F. Oliveira
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  2. Hudson W. P. Carvalho
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  3. Alessandro S. Nunes
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  4. Geraldo H. Silva
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  5. Vicente P. Campos
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  6. Helvécio M. S. Júnior
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  7. Alberto J. Cavalheiro
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Correspondence to Denilson F. Oliveira.

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Oliveira, D.F., Carvalho, H.W.P., Nunes, A.S. et al. The activity of amino acids produced by Paenibacillus macerans and from commercial sources against the root-knot nematode Meloidogyne exigua . Eur J Plant Pathol 124, 57–63 (2009). https://doi.org/10.1007/s10658-008-9392-0

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