Abstract
Echinacea purpurea plants showing yellowing/reddening and phyllody symptoms were observed in southern Bohemia. Transmission electron microscopy observations showed phytoplasmas in sieve cells of symptomatic plants, but not in healthy ones. Direct and nested polymerase chain reaction with phytoplasma specific primers and subsequent restriction fragment length polymorphism analyses of 16S rDNA as well as sequence analyses of the 16S–23S ribosomal operon (1789 bp) enabled the classification of the detected phytoplasmas in the aster yellows group, ribosomal subgroup 16SrI-C. Identical analyses of the transcription factor tuf gene and the ribosomal protein rpl22, rps3 genes were used for further classification, and revealed the affiliations of the phytoplasmas to the tufIC and rpIC subgroups, respectively. The presence of clover phyllody phytoplasmas in purple coneflowers is a new record for this species.
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References
Bertaccini, A., Fránová, J., Botti, S., & Tabanelli, D. (2005). Molecular characterization of phytoplasmas in lilies with fasciation in the Czech Republic. FEMS Microbiology Letters, 249, 79–85.
Hwang, S. F., Chang, K. F., Howard, R. J., Khadhair, A. H., Gaudiel, R. G., & Hiruki, C. (1997). First report of yellows phytoplasma disease in purple coneflower (Echinacea spp.) in Canada. Journal of Plant Diseases and Protection, 104, 182–192.
Jomantiene, R., Maas, J. L., Takeda, F., & Davis, R. E. (2002). Molecular identification and classification of strawberry phylloid fruit phytoplasma in group 16SrI, new subgroup R. Plant Disease, 86, 920.
Khadhair, A. H., Hwang, S. F., Chang, K. F., & Howard, R. J. (1997). Molecular identification of aster yellows phytoplasma in purple coneflower and monarda based on PCR amplification and RFLP analyses of 16S rDNA sequences. Journal of Plant Diseases and Protection, 104, 403–410.
Lee, I.-M., Gundersen, D. E., Davis, R. E., & Bartoszyk, I. M. (1998). Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. International Journal of Systematic Bacteriology, 48, 1153–1169.
Lee, I.-M., Gundersen-Rindal, D. E., Davis, R. E., Bottner, K. D., Marcone, C., & Seemüller, E. (2004). ‘Candidatus Phytoplasma asteris’, a novel phytoplasma taxon associated with aster yellows and related diseases. International Journal of Systematics and Evolutionary Microbiology, 54, 1037–1048.
Namba, S., Oyaizu, H., Kato, S., Iwanami, S., & Tsuchizaki, T. (1993). Phylogenetic diversity of phytopathogenic mycoplasmalike organisms. International Journal of Systematic Bacteriology, 43, 461–467.
Schneider, B., Gibb, K. S., & Seemüller, E. (1997). Sequence and RFLP analysis of the elongation factor Tu gene used in differentiation and classification of phytoplasmas. Microbiology, 143, 3381–3389.
Stanosz, G. R., Heimann, M. F., & Lee, I.-M. (1997). Purple coneflower is a host of the aster yellows phytoplasma. Plant Disease, 81, 424.
Acknowledgements
This work was funded by research grants from the GA ASCR nos. 1QS500510558 and AV0Z50510513. The authors are deeply indebted to Mrs J. Rakouská and Mrs. O. Šonková for their technical assistance.
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Fránová, J., Přibylová, J. & Petrzik, K. Purple coneflower with reddening and phyllody: a new host of clover phyllody phytoplasma. Eur J Plant Pathol 123, 85–90 (2009). https://doi.org/10.1007/s10658-008-9320-3
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DOI: https://doi.org/10.1007/s10658-008-9320-3