Abstract
Phosphites (Phi) are alkali metal salts of phosphorous acid, with the ability to protect plants against different pathogens. In this research, the effect of Phi applied to potato plants on severity of three important potato diseases in Argentina was assessed. Seed tubers and foliage of potato cvs Shepody and Kennebec were treated with Phi to assess effects on resistance against Phytophthora infestans, Fusarium solani and Rhizoctonia solani. Protection resulting from Phi treatment in seed tubers was high against P. infestans, intermediate against F. solani, and low against R. solani. In addition, seed tubers treated with calcium or potassium phosphites (CaPhi and KPhi, respectively) at 1% of commercial product emerged earlier than untreated ones. When Phi were foliarly applied two or four times at different doses, high levels of protection against P. infestans were achieved in both cultivars. Higher protection was observed in Kennebec when CaPhi was applied, while in Shepody this was true for KPhi. Expression of β-1,3-glucanases was induced at different times after treatment but no correlation between β-1,3-glucanases expression and foliar protection level was found. On the other hand, Phi positive protection effects did not produce negative effects in plant growth. Leaves from CaPhi-treated plants showed a darker green colour than leaves from control plants; also an increase in Rubisco protein and a delay in crop senescence was observed.
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References
Andreu, A. B., & Caldiz, D. O. (2004). Chemical control strategies for late blight management in Argentina. Phytopathology, 94, S4.
Andreu, A. B., Guevara, M. G., Wolski, E. A., Daleo, G. R., & Caldiz, D. O. (2006). Enhancement of natural disease resistance in potatoes by chemicals. Pest Management Science, 62, 162–170.
Bécot, S., Pajot, E., Le Corre, D., Monot, C., & Silué, D. (2000). Phytogard® (K2HPO3) induces localized resistance in cauliflower to downy mildew of crucifers. Crop Protection, 19, 417–425.
Bokshi, A. I., Morris, S. C., & Deverall, B. J. (2003). Effects of benzothiadiazole and acetylsalicylic acid on β-1,3-glucanase activity and disease resistance in potato. Plant Pathology, 52(1), 22–27.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248–254.
Caldiz, D. O. (2007). Producción, cosecha y almacenamiento de papa en la Argentina. BASF Argentina S.A.—McCain Argentina S.A. (1st ed.). Balcarce, Argentina.
Cooke, L. R., & Little, G. (1996). Foliar application of phosphonate formulations for the control of potato tuber blight. Brighton Crop Protection Conference, Pests Diseases, 1, 263–268.
Cooke, L. R., & Little, G. (2001). The effect of foliar application of phosphonate formulation on the susceptibility of potato tubers to late blight. Pest Management Science, 58, 17–25.
Escande, A. R., & Echandi, E. (1991). Protection of potato from Rhizoctonia canker with binucleate Rhizoctonia fungi. Plant Pathology, 40, 197–202.
Goth, R. W., & Keane, J. (1997). A detached-leaf method to evaluate late blight resistance in potato and tomato. American Potato Journal, 74, 347–352.
Gozzo, F. (2003). Systemic acquired resistance in crop protection: From Nature to a Chemical Approach. Journal of Agricultural and Food Chemistry, 51, 4487–4503.
Gozzo, F. (2004). Systemic acquired resistance in crop protection. Outlooks on Pest Management, 15, 20–23.
Guest, D., & Grant, B. R. (1991). The complex action of phosphonates as antifungal agents. Biological Reviews, 66, 159–187.
Johnson, D. A., Inglis, D. A., & Miller, J. S. (2004). Control of potato tubers rots caused by Oomycetes with foliar applications of phosphorous acid. Plant Disease, 88, 1153–1159.
Kombrink, E., Schöder, M., & Hahlbrock, K. (1988). Several “pathogenesis-related” proteins in potato are 1,3-β-glucanases and chitinases. Proceedings of the National Academy of Sciences USA, 85, 782–786.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685.
McDonald, A. E., Grant, B. R., & Plaxton, W. C. (2001). Phosphite (phosphorous acid): Its relevance in the environment and agriculture and influence on plant phosphate starvation response. Journal of Plant Nutrition, 24, 1505–1519.
McGrath, M. T. (2004). What are fungicides? The plant health instructor. The American Phytopathological Society. DOI 10.1094/PHI-I-2004-0825-01. http://www.apsnet.org/education/IntroPlantPath/Topics/fungicides/pdfs/CommonAndTradeFungicides.pdf. Last accessed: December 14, 2007.
Powelson, M. L., Ludy, R., Partipilo, H., Inglis, D. A., Gundersen, B., & Derie, M. (2002). Seed Borne Late Blight of Potato. Plant Health Progress, DOI 10.1094/PHP-2002-0129-01-HM. Last accessed: December 17, 2007
Smillie, R., Grant, B. R., & Guest, D. (1989). The mode of action of Phosphite: Evidences for both direct and indirect action modes of action on three Phytophthora spp. in plants. Phytopathology, 79, 921–926.
Sneh, B., Zeidan, M., Ichielevich-Auster, M., Barash, I., & Koltin, Y. (1986). Increased growth responses induced by a nonpathogenic isolate of Rhizoctonia solani. Canadian Journal of Botany, 64, 2372–2378.
Torres, H. (2002). Manual de las enfermedades más importantes de la papa en el Perú. Centro Internacional de la Papa (CIP). Lima, Perú: Ministerio de Agricultura.
Turner, B. M. (1986). Use of alkaline phosphatase-conjugated antibodies for detection of protein antigens on nitrocellulose. Methods in Enzymology, 121, 848–855.
Underwood, A. J. (1997). Experiments in ecology: their logical design and interpretation using analysis of variance. Cambridge, New York: Cambridge University Press.
Zar, J. H. (1999). Biostatistical analysis (4th ed.). Upper Saddle River, New York: Prentice-Hall.
Acknowledgements
This work was supported by Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC) and Universidad Nacional de Mar del Plata (UNMdP). McCain Argentina S.A. and Agro-EMCODI S.A. Andreu AB and Olivieri FP are established researchers from CONICET. Daleo GR is an established researcher of CIC. Caldiz DO is Agronomy Manager of the Agronomy Division, McCain Argentina S.A. Wolski EA and Lobato MC are a fellows of CONICET. González Altamiranda EA is student of UNMdP. The authors gratefully acknowledge Dr. Gregory A. Forbes from Centro Internacional de la Papa (CIP), and Dr. Lorenzo Lamattina and Dr. Mariana Feldman from Universidad Nacional de Mar del Plata for their useful and critical comments on the original version of this paper.
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Lobato, M.C., Olivieri, F.P., Altamiranda, E.A.G. et al. Phosphite compounds reduce disease severity in potato seed tubers and foliage. Eur J Plant Pathol 122, 349–358 (2008). https://doi.org/10.1007/s10658-008-9299-9
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DOI: https://doi.org/10.1007/s10658-008-9299-9