Abstract
Soil-borne cereal mosaic virus (SBCMV) causes a severe disease in susceptible cultivars of winter wheat. The virus is vectored by the soil-borne protist Polymyxa graminis. Experiments were conducted to investigate whether SBCMV RNA2 could persist in seed from SBCMV-infected susceptible cultivars of winter wheat. Over 7,000 seedlings were generated from seed collected from two cultivars of SBCMV-infected winter wheat. Seedlings were grown in a glasshouse compartment and batch tested for the presence of SBCMV using real-time RT-PCR. The majority of batches tested positive for SBCMV, indicating an RNA2 transmission rate of 1.8–9.4% in wheat. The presence of the virus was confirmed by amplifying and sequencing a larger (400 bp) fragment of viral RNA2 in a sub-set of the seedlings testing positive by real-time RT-PCR. Root extracts from this sub-set tested negative for P. graminis using real-time PCR. The implications for disease epidemiology of this virus are discussed.
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Acknowledgements
The authors wish to thank Richard Baker (CSL) for constructing the map of the outbreak sites and Theo Allnutt (CSL) for assistance with the calculations of infection rate. The authors would also like to thank the Plant Health Division of Defra for funding.
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The authors are British Civil Servants and as such their work is subject to British Crown Copyright. This means the exclusive copyright for the article cannot be transferred.
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Budge, G.E., Loram, J., Donovan, G. et al. RNA2 of Soil-borne cereal mosaic virus is detectable in plants of winter wheat grown from infected seeds. Eur J Plant Pathol 120, 97–102 (2008). https://doi.org/10.1007/s10658-007-9194-9
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DOI: https://doi.org/10.1007/s10658-007-9194-9