Abstract
Xanthomonas axonopodis pv. phaseoli and its variant fuscans are the causal agents of common bacterial blight of bean. Production of seeds is recommended in arid climates with the use of pathogen-free seeds. However, contamination of seeds still occurs in these seed production areas. To verify if low contamination levels of sown seeds could explain these field contaminations, we used seeds that were naturally contaminated with CFBP4834-R, a rifamycin-resistant X. axonopodis pv. phaseoli fuscous strain, to contaminate field plots at different rates. We also inoculated seeds to verify some parameters of plant colonization and seed transmission. In growth chambers, seedling contamination was always successful from seeds contaminated with CFBP4834-R having population sizes greater than 1 × 103 CFU seed−1 and were not successful below 1 × 102 CFU seed−1. In the greenhouse, the efficiency of contamination of seeds was not significantly different between contaminated plants that had a low or a high CFBP4834-R population size and reached between 40% and 52% whatever the origin of the inoculum (aerial or seed-borne). In field experiments, under low relative humidity, plots with 0.1–0.003% contamination rates or plots sown with seeds that were inoculated with low CFBP4834-R population sizes (1 × 102 and 1 × 104 CFU seed−1) led to an asymptomatic colonization of bean during the entire growing season with low CFBP4834-R population sizes. Seeds were contaminated both in primary and secondary foci. The contamination of seeds without symptom expression during the growing season represents a risk for eventual disease outbreaks.
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Aggour, A. R., Coyne, D. P., Vidaver, A. K., & Eskridge, K. M. (1989). Transmission of the common blight pathogen in bean seed. Journal of American Society of Horticultural Science, 114, 1002–1008.
Audy, P., Braat, C. E., Saindon, G., Huang, H. C., & Laroche, A. (1996). A rapid and sensitive PCR-based assay for concurrent detection of bacteria causing common and halo blights in bean seed. Phytopathology, 86, 361–366.
Cafati, C. R., & Saettler, A. W. (1980). Transmission of Xanthomonas phaseoli in seed of resistant and susceptible Phaseolus genotypes. Phytopathology, 70, 638–640.
Chan, J. W. Y. F., & Goodwin, P. H. (1999). Differentiation of Xanthomonas campestris pv. phaseoli from Xanthomonas campestris pv. phaseoli var. fuscans by PFGE and RFLP. European Journal of Plant Pathology, 105, 867–878.
Dow, J. M., Crossman, L., Findlay, K., He, Y. Q., Feng, J. X., & Tang, J. L. (2003). Biofilm dispersal in Xanthomonas campestris is controlled by cell–cell signaling and is required for full virulence to plants. Proceedings of the National Academy of Science of the United States of America, 100, 10995–11000.
Fininsa, C., & Tefera, T. (2001). Effect of primary inoculum sources of bean common bacterial blight on early epidemics, seed yield and quality aspects. International Journal of Pest Management, 47, 221–225.
Fininsa, C., & Yuen, J. (2002). Temporal progression of bean common bacterial blight (Xanthomonas campestris pv. phaseoli) in sole and intercropping systems. European Journal of Plant Pathology, 108, 485–495.
Gent, D. H., Lang, J. M., & Schwartz, H. F. (2005). Epiphytic survival of Xanthomonas axonopodis pv. allii and X. axonopodis pv. phaseoli on leguminous hosts and onion. Plant Disease, 89, 558–564.
Gilbertson, R. L., & Maxwell, D. P. (1992). Common bacterial blight of bean. In H. S. Chaube & J. Kumar (Eds.), Plant diseases of international importance. Diseases of vegetables and oil seed crop Vol. II (pp. 18–39). Englewood Cliffs, NJ: Prentice Hall.
Ishimaru, C., Eskridge, K. M., & Vidaver, A.K. (1991). Distribution analyses of naturally occurring epiphytic populations of Xanthomonas campestris pv. phaseoli on dry beans. Phytopathology, 81, 262–268.
International Seed Testing Association (2007). ISTA validated method 7–021: Detection of Xanthomonas axonopodis pv. phaseoli and Xanthomonas axonopodis pv. phaseoli var. fuscans on Phaseolus vulgaris. Retrieved March 2007, from http://www.seedtest.org/upload/cms/user/ISTAValidatedMethid7-021_2007_14.11.2006.pdf.
Jacques, M. A., Josi, K., Samson, R., & Darrasse, A. (2005). Xanthomonas axonopodis pv. phaseoli var. fuscans is aggregated in stable biofilm population sizes in the phyllosphere of field-grown beans. Applied and Environmental Microbiology, 71, 2008–2015.
Kaufman, P. H., & Leben, C. (1974). Soybean bacterial blight: Flower inoculation studies. Phytopathology, 64, 329–331.
Laurent, E. (1990). Deux années d’expérience sur la production française de semences de haricots indemnes de graisse: çà marche ! Bulletin fnams semences, 111, 53–54.
Mahuku, G. S., Jara, C., Henriquez, M. A., Gastellanos, C., & Cuasquer J. (2006). Genotypic Characterization of the Common Bean Bacterial Blight Pathogens, Xanthomonas axonopodis pv. phaseoli and Xanthomonas axonopodis pv. phaseoli var. fuscans by rep-PCR and PCR-RFLP of the Ribosomal Genes. Journal of Phytopathology, 154, 35–44.
Maude, R. B. (1996). Seed borne diseases and their control––principles & practice. UK: CAB International.
Maury, Y., Duby, C., Bossenec, J. M., & Boudazin, G. (1985). Group analysis using ELISA: Determination of the level of transmission of soybean mosaic virus in soybean seed. Agronomie, 5, 405–415.
Michael, T. E. (1994). The bean plant. In R. Hall (Ed.), Compendium of bean diseases (pp. 1–4). Saint Paul: APS Press.
Rudolph, K. (1993). Infection of the plant by Xanthomonas. In J. G. Swings, & E. L. Civerolo (Eds.), Xanthomonas (pp. 193–264). London, UK: Chapman & Hall.
Schaad, N. W. (1988). Inoculum thresholds of seedborne pathogens: Bacteria. Phytopathology, 78, 872–875.
Schaad, N. W., Postnikova, E., Lacy, G. H., Sechler, A., Agarkova, I., Stromberg, P. E., Stromberg, V. K., & Vidaver, A. K. (2005). Reclassification of Xanthomonas campestris pv. citri (ex Hasse 1915) Dye 1978 forms A, B/C/D, and E. As X. smithii subsp. citri (ex Hasse) sp. nov. nom. rev. comb. nov., and X. alfalfae subsp. citrumelo (ex Riker and Jones) Gabriel et al., 1989 sp. nov. nom. rev. comb. nov.; X. campestris pv. malvacearum (ex Smith 1901) Dye 1978 as X. smithii subsp. smithii nov. comb. nov. nom. nov.; X. campestris pv. alfalfae (ex Riker and Jones, 1935) Dye 1978 as X. alfalfae subsp. alfalfae (ex Riker et al., 1935) sp. nov. nom. rev.; and “var. fuscans” of X. campestris pv. phaseoli (ex Smith, 1987) Dye 1978 as X. fuscans subsp. fuscans sp. nov. Systematic and Applied Microbiology, 28, 494–518.
Sokal, R. R., & Rohlf, F. J. (1969). Biometry. The principles and practice of statistics in biological research. San Francisco, California: W. H. Freeman & Co.
Stall, R. E., Gottwald, T. R., Koizumi, M., & Schaad, N. W. (1993). Ecology of plant pathogenic xanthomonads. In J. G. Swings, & E. L. Civerolo (Eds.), Xanthomonas (pp. 265–299). London, UK: Chapman & Hall.
Taylor, J. D. (1970). The quantitative estimation of the infection of bean seed with Pseudomonas phaseolicola (Burkh.) Dowson. Annals of Applied Biology, 66, 29–36.
Trigalet, A., & Bidaud, P. (1978). Some aspects of epidemiology of bean halo blight. In Station de pathologie végétale et phytobactériologie (Ed.), Proceedings of the IVth International Conference on Plant Pathogenic Bacteria (pp.895–902). Angers, France: INRA.
Vauterin, L., Hoste, B., Kersters, K., & Swings, J. (1995). Reclassification of Xanthomonas. International Journal of Systematic Bacteriology, 45, 472–489.
Vauterin, L., Rademaker, J., & Swings, J. (2000). Synopsis on the taxonomy of the genus Xanthomonas. Phytopathology, 90, 677–682.
Vidaver, A. K. (1993). Xanthomonas campestris pv. phaseoli: cause of common bacterial blight of bean. In J. G. Swings, & E. L. Civerolo (Eds.), Xanthomonas (pp. 40–44). London, UK: Chapman & Hall.
Walcott, R.R., Gitaitis, R.D., & Castro, A. C. (2003). Role of blossoms in watermelon seed infestation by Acidovorax avenae subsp citrulli. Phytopathology, 93, 528–534.
Webster, D. M., Atkin, J. D., & Cross, J. E. (1983). Bacterial blights of snap beans and their control. Plant Disease, 67, 935–940.
Weller, D. M., & Saettler, A. W. (1980a). Colonization and distribution of Xanthomonas phaseoli and Xanthomonas phaseoli var. fuscans in field-grown navy beans. Phytopathology, 70, 500–506.
Weller, D. M., & Saettler, A. W. (1980b). Evaluation of seedborne Xanthomonas phaseoli and Xanthomonas phaseoli var. fuscans as primary inocula in bean blights. Phytopathology, 70, 148–152.
Zaumeyer, W. J. (1930). The bacterial blight of beans caused by Bacterium phaseoli. United States Department of Agriculture Technical Bulletin, 186.
Acknowledgments
This work was supported by grant from Conseil Régional Pays de la Loire. We thank J. Benard, P. Horeau, S. Hanteville, G. Orain and A. Lemarquand for bean field cultivations, V. Odeau and E. Laurent FNAMS, Brain sur l’Authion for production of inoculum, bean seed drying and sorting and M. Lemaire SNES, Angers for seed samplings. We thank K. Josi, M. Beaury, S. Houdault, D. Pé, C. Boëdo and A. -L. Girard for assistance, and C. Manceau for critical review of the manuscript.
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Darrasse, A., Bureau, C., Samson, R. et al. Contamination of bean seeds by Xanthomonas axonopodis pv. phaseoli associated with low bacterial densities in the phyllosphere under field and greenhouse conditions. Eur J Plant Pathol 119, 203–215 (2007). https://doi.org/10.1007/s10658-007-9164-2
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DOI: https://doi.org/10.1007/s10658-007-9164-2