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A High Multi-drug Resistance to Chemically Unrelated Oomycete Fungicides in Phytophthora infestans

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Abstract

Mutants of Phytophthora infestans with high resistance to the amidocarbamates iprovalicarb and benthiavalicarb and to the cyanoimidazole cyazofamid were isolated after UV-mutagenesis and selection on media containing one of the above fungicides. In vitro fungitoxicity tests showed that all resistant strains presented a highly reduced sensitivity to both cyazofamid and to the amidocarbamates. Cross-resistance studies with other oomycete fungicides from different chemical groups showed that the mutation(s) for resistance to iprovalicarb (IPV), benthiavalicarb (BVC) and cyazofamid (CZF) also greatly reduced the sensitivity of mutant strains to the phenylamide metalaxyl, acetamide cymoxanil, morpholine dimethomorph, benzamide zoxamide and to chlorothalonil. A lower reduction of sensitivity of mutant strains to the strobilurins azoxystrobin, kresoxim-methyl, pyraclostrobin and trifloxystrobin, azolones famoxadone and fenamidone and to antimycin A was observed. A resistance correlation was not apparent for the dithiocarbamate propineb and phenylpyridinamine fluazinam. Studies of fitness parameters in the wild-type and mutant strains of P. infestans showed that most resistant isolates had significantly reduced sporulation and sporangial germination, but not in the differentiation of sporangia into zoospores. Pathogenicity tests on tomato seedlings showed that most resistant isolates were significantly less pathogenic compared to the wild-type parent strain. However, experiments on the stability of the resistant phenotypes did not show a reduction in resistance when the mutants were grown for more than eleven generations on inhibitor-free medium. This is believed to be the first report of high level multi-drug resistance in fungal pathogens to chemically unrelated fungicides inhibiting different sites of cellular pathway.

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Acknowledgements

This research project was co-funded by European Social Fund and National Resources – EPEAEK II. BASF Agro Hellas, Bayer Crop Science Hellas and Syngenta Hellas are also gratefully acknowledged.

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Authors and Affiliations

  1. Laboratory of Pesticide Science, Agricultural University of Athens, Votanikos, 188 55, Athens, Greece

    Basil N. Ziogas, Anastasios N. Markoglou, Dimitrios I. Theodosiou, Ageliki Anagnostou & Stavroula Boutopoulou

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  1. Basil N. Ziogas
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  2. Anastasios N. Markoglou
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  3. Dimitrios I. Theodosiou
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  4. Ageliki Anagnostou
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  5. Stavroula Boutopoulou
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Correspondence to Basil N. Ziogas.

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Ziogas, B.N., Markoglou, A.N., Theodosiou, D.I. et al. A High Multi-drug Resistance to Chemically Unrelated Oomycete Fungicides in Phytophthora infestans . Eur J Plant Pathol 115, 283–292 (2006). https://doi.org/10.1007/s10658-006-9007-6

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  • DOI: https://doi.org/10.1007/s10658-006-9007-6

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