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Induction of oxidants in tomato leaves treated with DL-β-Amino butyric acid (BABA) and infected with Clavibacter michiganensis ssp. michiganensis

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Abstract

Bacterial canker is an economically important disease of tomato. Resistance induced by DL-β-Amino butyric acid against bacterial canker caused by Clavibacter michiganensis ssp. michiganensis in tomato plants was investigated. Different doses of DL-β-Amino butyric acid (250–1000 μg ml−1 doses) were tested on 3-week old plants inoculated with a 108 CFU  ml−1 bacterial suspension, and disease development was evaluated after inoculation and treatment. Although in vitro growth of the bacteria was not affected by DL-β-Amino butyric acid treatment, foliage sprays of 500 μg ml−1 DL-β-Amino butyric acid significantly suppressed disease development up to 54% by day 14 after inoculation at the four different doses tested. Bacterial populations were reduced by 84% in BABA-treated plants compared to water-treated plants by day 4 after inoculation. Inoculated BABA-treated plants showed significantly higher phenylalanine ammonia-lyase activity, peroxidase activity, and H2O2 concentration than inoculated water-treated plants during day 1 after treatment. These findings suggest that the DL-β-Amino butyric acid treatment resulted in an increase of these enzymes and in H2O2 concentration in planta, and was associated with induction of resistance to bacterial canker.

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Abbreviations

AOS:

active oxygen species

BABA:

DL-β-amino butyric acid

PAL:

phenylalanine ammonia-lyase

POX:

peroxidase

SAR:

systemic acquired resistance

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Authors and Affiliations

  1. West Mediterranean Agricultural Research Institute (WMARI), P.B. 35 07100, Antalya, Turkey

    Ömür Baysal

  2. Bornova Plant Protection Research Institute (BPRI), 35040, Bornova, Izmir, Turkey

    Y. Ziya Gürsoy, Hakan Örnek & Ahmet Duru

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  1. Ömür Baysal
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  2. Y. Ziya Gürsoy
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  3. Hakan Örnek
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  4. Ahmet Duru
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Correspondence to Ömür Baysal.

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Baysal, Ö., Gürsoy, Y.Z., Örnek, H. et al. Induction of oxidants in tomato leaves treated with DL-β-Amino butyric acid (BABA) and infected with Clavibacter michiganensis ssp. michiganensis . Eur J Plant Pathol 112, 361–369 (2005). https://doi.org/10.1007/s10658-005-6234-1

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