Abstract
Crinipellis perniciosa causes a serious disease of cacao known as witches’ broom (WB). Heritable resistance to witches’ broom has been used in cacao improvement programs. ‘SCA6’ and ‘SCA12’ are highly resistant and are the most commonly used parents in the breeding schemes. However, SCA hybrids are not resistant to witches’ broom in all production areas. Presumably, different populations of C. perniciosa cause these variable responses. Amplified fragment length polymorphism (AFLP) markers were used to assess variation and population structure in this pathogen. We examined 40 isolates of C. perniciosa and one isolate of Melanotus subcuneiformis. Nine of 64 primer pairs produced consistent and informative DNA amplification, and were used to screen all isolates. Fifteen haplotypes (AFLP fingerprints) were detected with 186 polymorphic markers. Cluster analysis grouped isolates of the C biotype (pathogenic on cacao) from Bolivia, Brazil, Ecuador and Trinidad together in a major cluster that was distinct from isolates of the S biotype (pathogenic on solanaceous hosts) and M. subcuneiformis. Isolates of the C biotype were divided further into well supported, country-specific groups. Segregation of AFLP alleles was not observed among basidiospore isolates from the same basidiome, broom, tree or field, supporting previous reports that the fungus did not outcross. The results corroborated prior conclusions that C. perniciosa was probably introduced into the Bahia state of Brazil from the Amazon basin. Representative isolates from the genetically distinct groups that were revealed will be used to examine pathogenic specialization in C. perniciosa and differential responses that have been reported in ‘SCA6’-derived germplasm.
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References
CJ Alexopoulos CW Mims M Blackwell (1996) Introductory Mycology EditionNumber4 John Wiley and Sons Inc. New York, USA
T Andebrhan A Figueria M Yamada J Cascardo DB Furtek (1999) ArticleTitleMolecular fingerprinting suggests two primary outbreaks of witches’ broom disease (Crinipellis perniciosa) of Theobroma cacao in Bahia, Brazil European Journal of Plant Pathology 105 167–175 Occurrence Handle1:CAS:528:DyaK1MXjvVCntbc%3D
T Andebrhan DB Furtek (1994) ArticleTitleRandom amplified polymorphic DNA (RAPD) analysis of Crinipellis perniciosa isolates from different hosts Plant Pathology 43 1020–1027 Occurrence Handle1:CAS:528:DyaK2MXktlahurc%3D
MCC Arruda MAS Ferreira RNG Miller MSS Felipe (2003a) ArticleTitleComparison of Crinipellis perniciosa isolates from Brazil by ERIC repetitive element sequence-based PCR genomic fingerprinting Plant Pathology 52 236–244
MCC Arruda RNG Miller MAS Ferreira MLV Resende MSS Felipe (2003b) ArticleTitleNuclear and mitochondrial rDNA variability in Crinipellis perniciosa from different geographic origins and hosts Mycological Research 107 25–37
Bowers JH, Bailey BA, Hebbar PK, Sanogo S and Lumsden RD (2001) The impact of plant diseases on world chocolate production. Online at: http://www.apsnet.org/online/feature/cacao/top.html. Plant Health Progress doi:10.1094/PHP-2001-0709-01-RV.
EE Cheesman (1944) ArticleTitleNotes on the nomenclature, classification and possible relationships of cacao populations Tropical Agriculture 21 144–159
JJ Doyle JL Doyle (1990) ArticleTitleIsolation of plant DNA from fresh tissue Focus 12 13–15
HC Evans (2002) Invasive neotropical pathogens of tree crops R Watling J Frankland M Ainsworth S Isaac C Robinson (Eds) Tropical Mycology, Vol. 2 Micromycetes CABI publishing Wallingford, Oxon, UK 83–112
L Excoffier PE Smouse J Quatro (1992) ArticleTitleAnalysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data Genetics 131 479–491 Occurrence Handle1:CAS:528:DyaK38XlsVCntro%3D Occurrence Handle1644282
FAO (2003) FAOSTAT online database at: <http://www.fao.org/default.htm>
GA Frias LH Purdy RA Schmidt (1995) ArticleTitle.An inoculation method for evaluating resistance of cacao to Crinipellis perniciosa Plant Disease 79 787–791
GW Griffith JN Hedger (1994) ArticleTitleThe breeding biology of biotypes of the witches’ broom pathogen of cocoa, Crinipellis perniciosa Heredity 72 278–289
CJ Jones KJ Edwards S Castaglione MO Winfield F Sala C Wiel Particlevan de G Bredemeijer B Vosman M Matthes A Daly R Brettschneider P Bettini M Buiatti E Maestri A Malcevschi N Marmiroli R Aert G Volcakaert J Rueda R Linacero A Vazques A Karp (1997) ArticleTitleReproducibility testing of RAPD, AFLP, and SSR markers in plants by a network of European laboratories Molecular Breeding 3 381–390 Occurrence Handle1:CAS:528:DyaK2sXntFensbg%3D
HA Laker (1989) ArticleTitleVariation in the cultural characteristics of isolates of Crinipellis perniciosa in Trinidad Turrialba 39 501–505
D Majer R Mithen BG Lewis P Vos RP Oliver (1996) ArticleTitleThe use of AFLP fingerprinting for the detection of genetic variation in fungi Mycological Research 9 1107–1111
Marita JM Nienhus J Pires JL Aitken WM (2001) ArticleTitleAnalysis of genetic diversity in Theobroma cacao with emphasis on witches’ broom resistance Crop Science 41 1305–1316
FM McGeary BEJ Wheeler (1988) ArticleTitleGrowth rates and mycelial interactions between isolates of Crinipellis perniciosa from cocoa Plant Pathology 37 489–498
Miller MP (1998) AMOVA-PREP 1.01 A program for the preparation of AMOVA input files from dominant-marker raw data. Northern Arizona Univ. Dept of Biological Sciences, mpm2@nauvax.ucc.nau.edu
JC Motamayor AM Risterucci PA Lopez CF Ortiz A Moreno C Lanaud (2002) ArticleTitleCacao domestication I: The origin of the cacao cultivated by the Mayas Heredity 89 380–386 Occurrence Handle1:CAS:528:DC%2BD38XotFWksL0%3D
M Nei W Li (1979) ArticleTitleMathematical model for studying genetic variation in terms of restriction endonucleases Proceedings of the National Academy of Sciences (USA) 76 5269–5273 Occurrence Handle1:CAS:528:DyaL3cXitVWn
FJ Pound (1938) Cacao and Witches’ Broom Disease of South America Port-of-Spain Trinidad
LH Purdy RA Schmidt (1996) ArticleTitleStatus of cacao witches’ broom: Biology, epidemiology, and management Annual Review of Phytopathology 34 573–594 Occurrence Handle1:CAS:528:DyaK28XlsFOhsbs%3D Occurrence Handle15012557
J Rincones LW Meinhardt BC Vidal GAG Pereira (2003) ArticleTitleElectrophoretic karyotype analysis of Crinipellis perniciosa, the causal agent of witches’ broom disease of Theobroma cacao Mycological Research 107 452–458 Occurrence Handle1:CAS:528:DC%2BD3sXktVensbo%3D Occurrence Handle12825518
VT Queiros CT Guimarães D Anhert I Schuster RT Daher MG Pereira VRM Miranda LL Loguercio EG Barros MA Moreira (2003) ArticleTitleIdentification of major QTL in cocoa (Theobroma cacao L.) associated with resistance to witches’ broom Plant Breeding 122 268–272
RJ Schnell JS Brown CT Olano EJ Power CA Krol DN Kuhn JC Motamayor (2003) ArticleTitleEvaluation of avocado germplasm using microsatellite markers Journal of the American Society for Horticultural Science 128 881–889 Occurrence Handle1:CAS:528:DC%2BD3sXpsVCrurw%3D
PW Tooley NRO O’Neill ED Goley MM Carras (2000) ArticleTitleAssessment of diversity in Claviceps africana and other Claviceps species by RAM and AFLP analyses Phytopathology 90 1126–1130 Occurrence Handle1:CAS:528:DC%2BD3cXnt1Knsbo%3D
BEJ Wheeler R Mepsted (1988) ArticleTitlePathogenic variability among isolates of Crinipellis perniciosa from cocoa (Theobroma cacao) Plant Pathology 37 475–488
MM Yamada T Andebrhan DB Furtek (1998) ArticleTitleGenetic variability among isolates of Crinipellis perniciosa from solanaceous hosts and their relationship to isolates from Theobroma cacao Agrotrópica 10 123–126
Yap IV and Nelson RJ (1996). WinBoot; a program for performing bootstrap analysis of binary data to determine the confidence limits of UPGMA- based dendrograms. IRRI Discussion Paper, Series No. 14, pp. 1–20
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Ploetz, R.C., Schnell, R.J., Ying, Z. et al. Analysis of molecular diversity in Crinipellis perniciosa with AFLP markers. Eur J Plant Pathol 111, 317–326 (2005). https://doi.org/10.1007/s10658-004-3821-5
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DOI: https://doi.org/10.1007/s10658-004-3821-5