Abstract
Multiplex polymerase chain reaction was used to identify the potato cyst nematodes in soil samples from the Ukraine. The results show the occurrence of Globodera pallida in the Uzhhorod region (Zakarpats’ka oblast’), where only G. rostochiensis had been previously reported. In the mixed potato cyst nematode (PCN) populations, G. pallida was less prevalent (2–5%) than G. rostochiensis (95–98%). A phylogenetic analysis based on ribosomal DNA internal transcribed spacer sequences showed that the Ukrainian population of G. pallida had >99% sequence identity with other G. pallida pa2/3 isolates from Europe. This study has demonstrated that polymerase chain reaction-mediated amplification of specific regions of the potato cyst nematode genome is not only highly effective as a species diagnostic tool but is also a sensitive method which can be used for taxonomic purposes with cyst collections which vary in age.
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Abbreviations
- ITS:
-
internal transcribed spacer
- PCN:
-
potato cyst nematode
- rDNA:
-
ribosomal DNA
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Pylypenko, L.A., Uehara, T., Phillips, M.S. et al. Identification of Globodera rostochiensis and G. pallida in the Ukraine by PCR. Eur J Plant Pathol 111, 39–46 (2005). https://doi.org/10.1007/s10658-004-2732-9
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DOI: https://doi.org/10.1007/s10658-004-2732-9