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A diagnostic medium for the semi-selective isolation and enumeration of Xanthomonas axonopodispv. vignicola

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Abstract

A semi-selective medium for isolation of Xanthomonas axonopodis pv. vignicola from cowpea (Vigna unguiculata) plant and soil samples was developed. Twelve carbon and five nitrogen sources were tested with four strains of X. axonopodispv.vignicola, and 25 antibiotics were screened against saprophytes. -cellobiose (10g) was selected as the optimal carbon source. Among the antibiotics, cefazoline inhibited growth of most of the saprophytes with little effect on strains of the pathogen. ,-methionine enhanced growth of X. axonopodispv.vignicola. Boric acid along with ammonium chloride suppressed growth of Pseudomonas fluorescens. The semi-selective medium designated as cefazoline-cellobiose-methionine (CCM) medium contained K2HPO4 1.34g, KH2PO4 0.4g, MgSO4 0.3g, H3BO3 0.2g, NH4Cl 1.0g, -cellobiose 10g, cycloheximide 0.2g, ,-methionine 1.0g, cefazoline 10mg and agar 14g per l of water (pH 7.2). Colonies of X. axonopodispv.vignicola on CCM medium were whitish, round, raised and 0.2–1.8mm in diameter 96h after incubation. CCM medium generally inhibited growth of Pantoea agglomerans, Bacillus subtilis and saprophytes isolated from cowpea leaves. Colonies of Pseudomonas fluorescens and a saprophytic bacterium, which were not completely suppressed by CCM, could be differentiated from X. axonopodispv.vignicola by their smaller size and different color. The CCM medium proved useful for isolation of X. axonopodispv.vignicola from cowpea plant and soil samples. This is the first report of a semi-selective medium developed for detection of X. axonopodispv.vignicola.

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References

  • Andrewes AG, Hertzberg S, Liaaen-Jensen S and Starr MP (1973)The Xanthomonas ''carotenoids ''-non-carotenoid, brominated aryl-polyene esters. Acta Chemica Scandinavica 27:2382-2395

    Google Scholar 

  • Batur H (1994)Ein neues Selektivmedium fu ¨r Xanthomonas campestris pv. pelargonii, den Erreger der Stengelfa ¨ule und der Blattfleckenkrankheit von Pelargonien. MSc Thesis, Georg-August-Universita ¨t, Go ¨ttingen, Germany

  • Burkholder WH (1944)Xanthomonas vignicola sp. nov. pathogenic on cowpeas and beans. Phytopathology 34:430-432

    Google Scholar 

  • Claflin LE, Vidaver AK and Sasser M (1987)MXP, a semi-selective medium for Xanthomonas campestris pv. phaseoli. Phytopathology 77:730-734

    Google Scholar 

  • Chun WWC and Alvarez AM (1983)A starch-methionine medium for Xanthomonas campestris pv. campestris from plant debris in soil. Plant Disease 67:632-635

    Google Scholar 

  • Fessehaie A, Wydra K and Rudolph K (1999)Development of a new semiselective medium for isolating Xanthomonas campestris pv. manihotis from plant material and soil. Phytopathology 89:591-597

    Google Scholar 

  • Kaiser WJ and Ramos AH (1979)Two bacterial diseases of cowpea in East Africa. Plant Disease Reporter 63:304-308

    Google Scholar 

  • Karyone K, Harada H, Kunita M and Takano T (1970) Cefazolin, a new semisynthetic cephalosporin antibiotic. Synthesis and chemical properties of Cefazolin. Journal of Antibiotics 23:131-136

    Google Scholar 

  • Khatri-Chhetri GB (1997)Pathological characterization of strains of Xanthomonas campestris pv. vignicola, incitant of cowpea bacterial blight and bacterial pustule, originating from different geographical areas. MSc thesis. Georg-August-Universita ¨t, Go ¨ttingen, Germany

    Google Scholar 

  • Khatri-Chhetri GB (1999)Detection and characterization of Xanthomonas campestris pv. vignicola strains, incitant of cowpea bacterial blight and pustule, and studies on genotype/ pathogen-interactions. PhD thesis. Georg-August-Universi-ta ¨t, Go ¨ttingen, Germany

    Google Scholar 

  • Khatri-Chhetri GB, Wydra K and Rudolph K (1998)Variability of strains of Xanthomonas campestris pv. vignicola, incitant of cowpea bacterial blight and bacterial pustule, collected from several African and other countries. In: Mahadevan A (ed)Plant Pathogenic Bacteria, (pp 296-302) Centre for Advanced Study in Botany, Madras, India

    Google Scholar 

  • Khatri-Chhetri G, Wydra K and Rudolph K (2003)Metabolic diversity of Xanthomonas axonopodis pv. vignicola, causal agent of cowpea bacterial blight and pustule. European Journal of Plant Pathology 109:851-860

    Google Scholar 

  • Kim HK (1982)Epidemiological, genetical and physiological studies of the bacterial leaf streak pathogen, Xanthomonas campestris pv. translucens (J. J. R. )Dowson. PhD thesis. Montana State University, Bozeman, USA

    Google Scholar 

  • Kishun R (1989)Appraisal of loss in yield of cowpea due to Xanthomonas campestris pv. vignicola. Indian Phytopathology 42:241-246

    Google Scholar 

  • Mohan SK and Schaad NW (1987)An improved agar plating assay for detecting Pseudomonas syringae pv. syringae and P. syringae pv. phaseolicola in contaminated bean seed. Phytopathology 77:1390-1395

    Google Scholar 

  • Norman D and Alvarez A (1989)A rapid method for presumptive identification of Xanthomonas campestris pv. die. enbachiae and other xanthomonads. Plant Disease 73: 654-658

    Google Scholar 

  • Patel PN and Singh D (1984)New bacterial blight resistant vegetable cowpeas in India. Tropical Grain Legume Bulletin 29:14-18

    Google Scholar 

  • Raj S and Patel PN (1977)Studies on resistance in crops to bacterial diseases in India. IX. Sources of multiple disease resistance in cowpea. Indian Phytopathology 30:207-212

    Google Scholar 

  • Randhawa P and Schaad NW (1984)Selective isolation of Xanthomonas campestris pv. campestris from crucifer seeds. Phytopathology 74:268-272

    Google Scholar 

  • SAS Institute Inc (1997)SAS/STAT Software:Changes and Enhancements, through Release 6. 12, SAS Institute Inc, Cary, North Carolina, USA

  • Schaad NW (1988)Laboratory Guide for Identification of Plant Pathogenic Bacteria, 2nd ed. APS Press, St Paul, MN, USA

    Google Scholar 

  • Schaad NW and Forster RL (1985)A semi-selective agar medium for isolating Xanthomonas campestris pv. translucens from wheat seeds. Phytopathology 75:260-263

    Google Scholar 

  • Schaad NW and Stall RE (1988)Xanthomonas. In:Schaad NW (ed)Laboratory Guide for Identification of Plant Pathogenic Bacteria (pp 81-94)2nd ed. APS, St Paul, MN, USA

    Google Scholar 

  • Severin V and Stancescu C (1990)Bacterial blight of cowpea in Romania. Buletinul de Protectia Plantelor 1:3-8

    Google Scholar 

  • Sherwin HS and Lefebvre CL (1951)Reaction of cowpea varieties to bacterial canker. Plant Disease Reporter 35:302-317

    Google Scholar 

  • Sikirou R (1999a)Inoculum sources of Xanthomonas campestris pv. vignicola strains, incitant of cowpea bacterial blight and bacterial pustule, and identification of hosts besides Vigna unguiculata. MSc thesis. Georg-August-Universita ¨t, Go ¨ttin-gen, Germany

    Google Scholar 

  • Sikirou R (1999b)Epidemiological investigations and development of integrated control methods of bacterial blight of cowpea caused by Xanthomonas campestris pv. vignicola. PhD thesis. Georg-August-Universita ¨t, Go ¨ttingen, Germany

    Google Scholar 

  • Sikirou R, Wydra K and Rudolph K (2001)Selection of cowpea genotypes resistant to bacterial blight caused by Xanthomonas campestris pv. vignicola. In:DeBoer SH (ed) Plant Pathogenic Bacteria (pp 309-314)Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Stead DE (1990)Preservation of bacteria. In:Klement Z, Rudolph K and Sands DC (eds)Methods in Phytobacteri-ology (pp 275-278)Akademiai Kiado, Budapest, Hungary

    Google Scholar 

  • Toussaint V, Morris CE and Carisse O (2001)A new semi-selective medium for Xanthomonas campestris pv. vitians, the causal agent of bacterial leaf spot of lettuce. Plant Disease 85: 131-136

    Google Scholar 

  • Vakili NG, Kaiser WJ, Perez JE and Cortes-Monllor A (1975) Bacterial blight of beans caused by two Xanthomonas pathogenic types from Puerto Rico. Phytopathology 65: 401-403

    Google Scholar 

  • Vauterin L, Hoste B, Kersters K and Swings J (1995) Reclassification of Xanthomonas. International Journal of Systematic Bacteriology 45:472-489

    Google Scholar 

  • Verdier V, Assigbe ´tse K, Khatri-Chhetri GB, Wydra K, Rudolph K and Geiger JP (1998)Molecular characterization of the incitant of cowpea bacterial blight and pustule Xanthomonas campestris pv. vignicola. European Journal of Plant Pathology 104:595-602

    Google Scholar 

  • Wang ZK, Comstock JC, Hatziloukas E and Schaad NW (1999)Comparison of PCR, BIO-PCR, DIA, ELISA and isolation on semi-selective medium for detection of Xantho-monas albilineans, the causal agent of leaf scald of sugarcane. Plant Pathology 48:245-252

    Google Scholar 

  • Wydra K, Fanou A, Sikirou R, Zandjanakou M, Zinsou V and Rudolph K (2001)Integrated control of bacterial diseases of cassava and cowpea in West Africa. In:DeBoer SH (ed) Plant Pathogenic Bacteria (pp 280-287)Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

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Authors and Affiliations

  1. Institute of Plant Pathology and Plant Protection, University of Göttingen, Grisebachstr. 6, Göttingen, 37077, Germany

    Kerstin Wydra, Gopal Khatri-chhetri, Athanassios Mavridis & Klaus Rudolph

  2. International Institute of Tropical Agriculture, B.P. 08 Tri Postal, 0932, Cotonou, Benin

    Kerstin Wydra & Rachidatou Sikirou

  3. Institute of Plant Diseases and Plant Protection, University of Hannover, Herrenhäuser Str. 2, Hannover, 30419, Germany

    Kerstin Wydra

Authors
  1. Kerstin Wydra
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  2. Gopal Khatri-chhetri
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  3. Athanassios Mavridis
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  4. Rachidatou Sikirou
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  5. Klaus Rudolph
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Wydra, K., Khatri-chhetri, G., Mavridis, A. et al. A diagnostic medium for the semi-selective isolation and enumeration of Xanthomonas axonopodispv. vignicola . European Journal of Plant Pathology 110, 991–1001 (2004). https://doi.org/10.1007/s10658-004-0812-5

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