Abstract
The clarification of possible exposure sources of multiple metals to identify associations between metal doses and urothelial carcinoma (UC) risk is currently limited in the literature. We sought to identify the exposure sources of 10 metals (Vanadium, chromium, manganese, cobalt, nickel, copper, zinc, arsenic, cadmium, and lead) using principal component analysis (PCA) and then linked various principal component (PC) scores with environmental characteristics, including smoking-related indices, PM2.5, and distance to the nearest bus station. In addition, urinary 8-hydroxy-2’-deoxyguanosine (8-OHdG) and DNA hypomethylation markers (5-methyl-2’-deoxycytidine levels; %5-MedC) were investigated in combination with UC risks. We conducted this hospital-based case control study in 359 UC patients with histologically confirmed disease and 718 controls. All data were collected from face-to-face interviews and medical records. Approximately 6 mL blood was collected from participants for analysis of multiple heavy metal and DNA methylation in leukocyte DNA. Further, a 20 mL urine sample was collected to measure urinary cotinine and 8-OHdG levels. In addition, average values for PM2.5 for individual resident were calculated using the hybrid kriging/land-use regression model. In UC patients, significantly higher cobalt, nickel, copper, arsenic, and cadmium (μg/L) levels were observed in blood when compared with controls. Three PCs with eigenvalues > 1 accounted for 24.3, 15.8, and 10.7% of UC patients, and 26.9, 16.7, and 11.1% of controls, respectively. Environmental metal sources in major clusters were potentially associated with industrial activities and traffic emissions (PC1), smoking (PC2), and food consumption, including vitamin supplements (PC3). Multiple metal doses were linked with incremental urinary 8-OHdG and DNA hypomethylation biomarkers. For individuals with high PC1 and PC2 scores, both displayed an approximate 1.2-fold risk for UC with DNA hypomethylation.
In conclusion, we provide a foundation for health education and risk communication strategies to limit metal exposure in environment, so that UC risks can be improved potentially.
Data Availability Statement
Data used and analyzed in the manuscript will be made available upon request.
References
Afridi, H. I., Talpur, F. N., Kazi, T. G., & Brabazon, D. (2015). Effect of trace and toxic elements of different brands of cigarettes on the essential elemental status of irish referent and diabetic mellitus consumers. Biological Trace Element Research, 167, 209–224.
Ali, M. U., Liu, G. J., Yousaf, B., Ullah, H., Abbas, Q., Munir, M. A. M., & Irshad, S. (2019). Biomonitoring and health risks assessment of trace elements in various age- and gender-groups exposed to road dust in habitable urban-industrial areas of Hefei, China. Environmental Pollution, 244, 809–817.
Chang, C. H., Liu, C. S., Liu, H. J., Huang, C. P., Huang, C. Y., Hsu, H. T., Liou, S. H., & Chung, C. J. (2016). Association between levels of urinary heavy metals and increased risk of urothelial carcinoma. International Journal of Urology, 23, 233–239.
Charron, A., Polo-Rehn, L., Besombes, J. L., Golly, B., Buisson, C., Chanut, H., Marchand, N., Guillaud, G., & Jaffrezo, J. L. (2019). Identification and quantification of particulate tracers of exhaust and non-exhaust vehicle emissions. Atmospheric Chemistry and Physics, 19, 5187–5207.
Chen, C. J., Hsu, L. I., Wang, C. H., Shih, W. L., Hsu, Y. H., Tseng, M. P., Lin, Y. C., Chou, W. L., Chen, C. Y., Lee, C. Y., Wang, L. H., Cheng, Y. C., Chen, C. L., Chen, S. Y., Wang, Y. H., Hsueh, Y. M., Chiou, H. Y., & Wu, M. M. (2005). Biomarkers of exposure, effect, and susceptibility of arsenic-induced health hazards in Taiwan. Toxicology and Applied Pharmacology, 206, 198–206.
Chen, C. Y., Jhou, Y. T., Lee, H. L., & Lin, Y. W. (2016). Simultaneous, rapid, and sensitive quantification of 8-hydroxy-2’-deoxyguanosine and cotinine in human urine by on-line solid-phase extraction LC-MS/MS: Correlation with tobacco exposure biomarkers NNAL. Analytical and Bioanalytical Chemistry, 408, 6295–6306.
Chung, C. J., Chang, C. H., Liou, S. H., Liu, C. S., Liu, H. J., Hsu, L. C., Chen, J. S., & Lee, H. L. (2017). Relationships among DNA hypomethylation, Cd, and Pb exposure and risk of cigarette smoking-related urothelial carcinoma. Toxicology and Applied Pharmacology, 316, 107–113.
Chung, C. J., Chang, C. H., Liu, C. S., Huang, C. P., Chang, Y. H., Chien, S. N., Tsai, P. H., & Hsieh, H. A. (2014). Association of DNA methyltransferases 3A and 3B polymorphisms, and plasma folate levels with the risk of urothelial carcinoma. PLoS ONE, 9, e104968.
Chung, C. J., Lee, H. L., Chang, C. H., Chang, H., Liu, C. S., Jung, W. T., Liu, H. J., Liou, S. H., Chung, M. C., & Hsueh, Y. M. (2019). Measurement of urinary arsenic profiles and DNA hypomethylation in a case-control study of urothelial carcinoma. Archives of Toxicology, 93, 2155–2164.
Chung, C. J., Wu, C. D., Hwang, B. F., Wu, C. C., Huang, P. H., Ho, C. T., & Hsu, H. T. (2020). Effects of ambient PM2.5 and particle-bound metals on the healthy residents living near an electric arc furnace: A community- based study. Science of the Total Environment, 728, 138799.
Csalari, J., & Szantai, K. (2002). Transfer rate of cadmium, lead, zinc and iron from the tobacco-cut of the most popular Hungarian cigarette brands to the combustion products. Acta Alimentaria, 31, 279–288.
Dodmane, P. R., Arnold, L. L., Muirhead, D. E., Suzuki, S., Yokohira, M., Pennington, K. L., Dave, B. J., Lu, X., Le, X. C., & Cohen, S. M. (2014). Characterization of intracellular inclusions in the urothelium of mice exposed to inorganic arsenic. Toxicological Sciences, 137, 36–46.
Drago, G., Perrino, C., Canepari, S., Ruggieri, S., L’Abbate, L., Longo, V., Colombo, P., Frasca, D., Balzan, M., Cuttitta, G., Scaccianoce, G., Piva, G., Bucchieri, S., Melis, M., Viegi, G., Cibella, F., Balzan, M., Bilocca, D., Borg, C., … Scaccianoce, G. (2018). Relationship between domestic smoking and metals and rare earth elements concentration in indoor PM2.5. Environmental Research, 165, 71–80. https://doi.org/10.1016/j.envres.2018.03.026
Fiala, M., & Hwang, H. M. (2021). Influence of highway pavement on metals in road dust: A case study in Houston, Texas. Water Air and Soil Pollution, 232, 1–12.
Galazyn-Sidorczuk, M., Brzoska, M. M., & Moniuszko-Jakoniuk, J. (2008). Estimation of Polish cigarettes contamination with cadmium and lead, and exposure to these metals via smoking. Environmental Monitoring and Assessment, 137, 481–493.
Gellein, K., Lierhagen, S., Brevik, P. S., Teigen, M., Kaur, P., Singh, T., Flaten, T. P., & Syversen, T. (2008). Trace element profiles in single strands of human hair determined by HR-ICP-MS. Biological Trace Element Research, 123, 250–260.
George, J., Masto, R. E., Ram, L. C., Das, T. B., Rout, T. K., & Mohan, M. (2015). Human exposure risks for metals in soil near a coal-fired power-generating plant. Archives of Environmental Contamination and Toxicology, 68, 451–461.
Golabek, T., Socha, K., Kudelski, J., Darewicz, B., Markiewicz-Zukowska, R., Chlosta, P., & Borawska, M. (2017). Chromium in urothelial carcinoma of the bladder. Annals of Agricultural and Environmental Medicine, 24, 602–605.
Guo, X., Su, W., Li, N., Song, Q., Wang, H., Liang, Q., Li, Y., Lowe, S., Bentley, R., Zhou, Z., Song, E. J., Cheng, C., Zhou, Q., & Sun, C. (2022). Association of urinary or blood heavy metals and mortality from all causes, cardiovascular disease, and cancer in the general population: A systematic review and meta-analysis of cohort studies. Environmental Science and Pollution Research International, 29, 67483–67503.
Ha, S. D., Hu, H., Roth, J., Kan, H. D., & Xu, X. H. (2015). Associations between residential proximity to power plants and adverse birth outcomes. American Journal of Epidemiology, 182, 215–224.
Haines, D. A., Saravanabhavan, G., Werry, K., & Khoury, C. (2017). An overview of human biomonitoring of environmental chemicals in the Canadian health measures survey: 2007–2019. International Journal of Hygiene and Environmental Health, 220, 13–28.
Han, B. C., Jeng, W. L., Hung, T. C., & Jeng, M. S. (1994). Copper intake and health threat by consuming seafood from copper-contaminated coastal environments in Taiwan. Environmental Toxicology and Chemistry, 13, 775–780.
Hanahan, D., & Weinberg, R. A. (2011). Hallmarks of cancer: The next generation. Cell, 144, 646–674.
Henneman, L. R. F., Choirat, C., & Zigler, C. M. (2019). Accountability assessment of health improvements in the United States associated with reduced coal emissions between 2005 and 2012. Epidemiology, 30, 477–485.
Howard, D. J., Ota, R. B., Briggs, L. A., Hampton, M., & Pritsos, C. A. (1998). Environmental tobacco smoke in the workplace induces oxidative stress in employees, including increased production of 8-hydroxy-2’-deoxyguanosine. Cancer Epidemiology Biomarkers & Prevention, 7, 141–146.
Huang, B. F., Chang, Y. C., Han, A. L., & Hsu, H. T. (2018). Metal composition of ambient PM2.5 influences the pulmonary function of schoolchildren: A case study of school located nearby of an electric arc furnace factory. Toxicology and Industrial Health, 34, 253–261.
Hulskotte, J. H. J., Roskam, G. D., & van der Gon, H. A. C. D. (2014). Elemental composition of current automotive braking materials and derived air emission factors. Atmospheric Environment, 99, 436–445.
Jain, R. B. (2019). Concentrations of cadmium, lead, and mercury in blood among US cigarettes, cigars, electronic cigarettes, and dual cigarette-e-cigarette users. Environmental Pollution, 251, 970–974.
Johansson, C., Norman, M., & Burman, L. (2009). Road traffic emission factors for heavy metals. Atmospheric Environment, 43, 4681–4688.
Kaiser, H. F. (1958). The varimax criterion for analytic rotation in factor analysis. Psychometrika, 23, 187–200.
Lewis, R. C., Gaffney, S. H., Le, M. H., Unice, K. M., & Paustenbach, D. J. (2012). Airborne concentrations of metals and total dust during solid catalyst loading and unloading operations at a petroleum refinery. International Journal of Hygiene and Environmental Health, 215, 514–521.
Lin, Y. J., Ling, M. P., Chen, S. C., Chen, W. Y., Hsieh, N. H., Cheng, Y. H., You, S. H., Chou, W. C., Lin, M. C., & Liao, C. M. (2017). Mixture risk assessment due to ingestion of arsenic, copper, and zinc from milkfish farmed in contaminated coastal areas. Environmental Science and Pollution Research International, 24, 14616–14626.
Lini, R. S., Aguera, R. G., Hoeltgebaum, D., Paniz, F. P., Pedron, T., Capelari, S., Monteiro, L. R., Machinski, M., Nerilo, S. B., Batista, B. L., de Oliveira, M. L. F., & Mossini, S. A. G. (2021). Elemental plasma content and urinary excretion in vineyard farmers occupationally exposed to pesticides in southern Brazil. Environmental Science and Pollution Research, 28, 51841–51853.
MoghaddamHosseini, V., Dowlatabadi, A., Najafi, M. L., Ghalenovi, M., Pajohanfar, N. S., Ghezi, S., Mehrabadi, S., Estiri, E. H., & Miri, M. (2022). Association of traffic-related air pollution with Newborn’s anthropometric indexes at birth. Environmental Research, 204, 112000.
Mostafa, M. G., & Cherry, N. (2015). Arsenic in drinking water, transition cell cancer and chronic cystitis in rural Bangladesh. International Journal of Environmental Research and Public Health, 12, 13739–13749.
Mugford, C., Boylstein, R., & Gibbs, J. L. (2017). Elemental properties of coal slag and measured airborne exposures at two coal slag processing facilities. Journal of Occupational and Environmental Hygiene, 14, 360–367.
Nisse, C., Tagne-Fotso, R., Howsam, M., Richeval, C., Labat, L., & Leroyer, A. (2017). Blood and urinary levels of metals and metalloids in the general adult population of Northern France: The IMEPOGE study, 2008–2010. International Journal of Hygiene and Environmental Health, 220(2), 341–363. https://doi.org/10.1016/j.ijheh.2016.09.020
Pu, Y. S., Yang, S. M., Huang, Y. K., Chung, C. J., Huang, S. K., Chiu, A. W., Yang, M. H., Chen, C. J., & Hsueh, Y. M. (2007). Urinary arsenic profile affects the risk of urothelial carcinoma even at low arsenic exposure. Toxicology and Applied Pharmacology, 218, 99–106.
Rajfur, M., Świsłowski, P., Nowainski, F., & Śmiechowicz, B. (2018). Mosses as biomonitor of air pollution with analytes originating from tobacco smoke. Chemistry-Didactics-Ecology-Metrology, 23, 127–136.
Saha, N., Rahman, M. S., Jolly, Y. N., Rahman, A., Sattar, M. A., & Hai, M. A. (2016). Spatial distribution and contamination assessment of six heavy metals in soils and their transfer into mature tobacco plants in Kushtia District, Bangladesh. Environmental Science and Pollution Research International, 23, 3414–3426.
Shoari, N., Heydari, S., & Blangiardo, M. (2022). School neighbourhood and compliance with WHO-recommended annual NO(2) guideline: A case study of Greater London. Science of the Total Environment, 803, 150038.
Sobolev, N., Aksenov, A., Sorokina, T., Chashchin, V., Ellingsen, D. G., Nieboer, E., Varakina, Y., Veselkina, E., Kotsur, D., & Thomassen, Y. (2019). Essential and non-essential trace elements in fish consumed by indigenous peoples of the European Russian Arctic. Environmental Pollution, 253, 966–973.
Stauber, J. M., Zhang, S., Gvozdik, N., Jiang, Y., Avena, L., Stevenson, K. J., & Cummins, C. C. (2018). Cobalt and Vanadium Trimetaphosphate Polyanions: synthesis, characterization, and electrochemical evaluation for non-aqueous redox-flow battery applications. Journal of the American Chemical Society, 140, 538–541.
Stepien, M., Hughes, D. J., Hybsier, S., Bamia, C., Tjonneland, A., Overvad, K., Affret, A., His, M., Boutron-Ruault, M. C., Katzke, V., Kuhn, T., Aleksandrova, K., Trichopoulou, A., Lagiou, P., Orfanos, P., Palli, D., Sieri, S., Tumino, R., Ricceri, F., … Jenab, M. (2017). Circulating copper and zinc levels and risk of hepatobiliary cancers in Europeans. British Journal of Cancer, 116, 688–696.
Sundaray, S. K., Nayak, B. B., Lin, S., & Bhatta, D. (2011). Geochemical speciation and risk assessment of heavy metals in the river estuarine sediments–a case study: Mahanadi basin, India. Journal of Hazardous Materials, 186, 1837–1846.
Świsłowski, P., Smiechowicz, B., & Rajfur, M. (2022b). Effects of tobacco smoke on indoor air quality: The use of mosses in biomonitoring. Journal of Environmental Health Science and Engineering, 20, 485–493.
Świsłowski, P., Vergel, K., Zinicovscaia, I., Rajfur, M., & Waclawek, M. (2022). Mosses as a biomonitor to identify elements released into the air as a result of car workshop activities. Ecological Indicators, 138, 108849.
Tomei, F., Rosati, M. V., Ciarrocca, M., Marchetti, M. R., Baccolo, T. P., Anzelmo, V., & Tomao, E. (2004). Urban pollution and nickel concentration in serum. International Journal of Environmental Health Research, 14, 65–74.
Tsai, J. W., Ju, Y. R., Huang, Y. H., Deng, Y. S., Chen, W. Y., Wu, C. C., & Liao, C. M. (2013). Toxicokinetics of tilapia following high exposure to waterborne and dietary copper and implications for coping mechanisms. Environmental Science and Pollution Research International, 20, 3771–3780.
Tsai, P. J., Young, L. H., Hwang, B. F., Lin, M. Y., Chen, Y. C., & Hsu, H. T. (2020). Source and health risk apportionment for PM2.5 collected in Sha-Lu area. Taiwan. Atmospheric Pollution Research, 11, 851–858.
Valko, M., Jomova, K., Rhodes, C. J., Kuca, K., & Musilek, K. (2016). Redox- and non-redox-metal-induced formation of free radicals and their role in human disease. Archives of Toxicology, 90, 1–37.
Vardoulakis, S., Giagloglou, E., Steinle, S., Davis, A., Sleeuwenhoek, A., Galea, K. S., Dixon, K., & Crawford, J. O. (2020). Indoor exposure to selected air pollutants in the home environment: A systematic review. International Journal of Environmental Research and Public Health, 17, 8972.
Vasiliu, D., Bucse, A., Lupascu, N., Ispas, B., Gheablau, C., & Stanescu, I. (2020). Assessment of the metal pollution in surface sediments of coastal Tasaul Lake (Romania). Environmental Monitoring and Assessment, 192, 749.
Wu, C. D., Zeng, Y. T., & Lung, S. C. (2018). A hybrid kriging/land-use regression model to assess PM2.5 spatial-temporal variability. Science of the Total Environment, 645, 1456–1464.
Xu, P. W., Chen, Y., He, S. L., Chen, W. Z., Wu, L. Z., Xu, D. D., Chen, Z. J., Wang, X. F., & Lou, X. M. (2020). A follow-up study on the characterization and health risk assessment of heavy metals in ambient air particles emitted from a municipal waste incinerator in Zhejiang China. Chemosphere, 246, 125777.
Yang, Y. W., Liou, S. H., Hsueh, Y. M., Lyu, W. S., Liu, C. S., Liu, H. J., Chung, M. C., Hung, P. H., & Chung, C. J. (2018). Risk of Alzheimer’s disease with metal concentrations in whole blood and urine: A case-control study using propensity score matching. Toxicology and Applied Pharmacology, 356, 8–14.
Zeegers, M. P., Kellen, E., Buntinx, F., & van den Brandt, P. A. (2004). The association between smoking, beverage consumption, diet and bladder cancer: A systematic literature review. World Journal of Urology, 21, 392–401.
Zhang, Y., Liu, Y., Chen, J., Guo, Q., Wang, T., & Pang, H. (2014). Cobalt vanadium oxide thin nanoplates: Primary electrochemical capacitor application. Scientific Reports, 4, 5687.
Acknowledgements
This study was supported by grants from the National Science Council (NSC 101-2314-B-039-025-MY3 and MOST 108-2221-E-039-009-MY3) and China Medical University and Hospital (DMR-105-049, CMU106-S-20, CMU107-S-06, and CMU108-MF-94). The funders did not participate in the study design, data collection and analysis, publication decision, or manuscript preparation.
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MCC, HTH, HLL, CHC, CDW, CSL, and CJC conceived and designed experiments, HTH and CJC conducted experiments, HTH and CJC analyzed data, MCC, HTH, HLL, CHC, CDW, CSL, and CJC contributed material, and HTH and CJC drafted manuscript.
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Chung, CJ., Lee, HL., Chang, CH. et al. Determination of potential sources of heavy metals in patients with urothelial carcinoma in central Taiwan: a biomonitoring case–control study. Environ Geochem Health 45, 5401–5414 (2023). https://doi.org/10.1007/s10653-023-01481-3
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DOI: https://doi.org/10.1007/s10653-023-01481-3