Abstract
Here we describe a novel, inexpensive and simple method for preserving RNA that reduces handling stress in aquatic invertebrates following ecotoxicogenomic experimentation. The application of the method is based on transcriptomic experiments conducted on Daphnia magna, but may easily be applied on a range of other aquatic organisms of a particular size with e.g. amphipod Gammarus pulex representing an upper size limit. We explain in detail how to apply this new method, named the “Cylindrical Sieve (CS) system”, and highlight its advantages and disadvantages.
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Many thanks to Andy Conisbee and Steve Middleton for technical assistance, and two anonymous reviewers for their valuable comments on the manuscript.
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Heckmann, LH., Bouetard, A., Hill, C.J. et al. A simple and rapid method for preserving RNA of aquatic invertebrates for ecotoxicogenomics. Ecotoxicology 16, 445–447 (2007). https://doi.org/10.1007/s10646-007-0148-y
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DOI: https://doi.org/10.1007/s10646-007-0148-y