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Recommendations for a Toxicological Screening ERG Procedure in Laboratory Animals

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Abstract

Electroretinography, using laboratory animals, is a commonly used technique for determining the retinal toxicity of chemical agents. In this paper, guidelines for performing this test are provided. The physiologic basis for visual testing is presented with attention to inter-species differences. Technical aspects of animal recordings are reviewed, including animal preparation, stimulation, signal conditioning, recording and data analysis. Finally, suggested protocols for recording in diurnal and nocturnal species are presented.

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Authors and Affiliations

  1. Clinique Vétérinaire Voltaire, 119 Boulevard Voltaire, 92600, Asnières, France

    Serge G. Rosolen

  2. Laboratoire de physiopathologie cellulaire et moléculaire de la rétine, INSERM U-592, Hôpital Saint-Antoine, Bâtiment Kourilsky, 184 rue du Faubourg Saint-Antoine, 75571, Paris, France

    Serge G. Rosolen, Florence Rigaudière & Jean-François Le Gargasson

  3. Pfizer Global Research & Development, Ann Arbor, MI, USA

    Mitchell G. Brigell

Authors
  1. Serge G. Rosolen
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  2. Florence Rigaudière
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  3. Jean-François Le Gargasson
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  4. Mitchell G. Brigell
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Rosolen, S.G., Rigaudière, F., Gargasson, JF.L. et al. Recommendations for a Toxicological Screening ERG Procedure in Laboratory Animals. Doc Ophthalmol 110, 57–66 (2005). https://doi.org/10.1007/s10633-005-7344-y

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  • DOI: https://doi.org/10.1007/s10633-005-7344-y

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