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Hsa-circRNA-103809 Promotes Hepatocellular Carcinoma Development via MicroRNA-1270/PLAG1 Like Zinc Finger 2 Axis

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Abstract

Background

Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related death in the worldwide. A great number of reports manifested that circular RNA hsa-circRNA-103809 (circRNA-103809) could work in several cancers.

Aims

This study aimed to explore the function and mechanism of circRNA-103809 in HCC.

Methods

Gene expressions were detected by quantitative real-time polymerase chain reaction. Colony formation, cell counting kit-8, transwell and wound healing assays were implemented to check the role of circRNA-103809 in HCC. Subcellular fractionation analysis was designed to figure out the cellular location of circRNA-103809. Luciferase reporter assay and RNA pull down assay were employed to verify the relationships among RNAs.

Results

CircRNA-103809 was highly expressed in HCC cell lines. After interfering circRNA-103809, the proliferation, migration, invasion and epithelial-to-mesenchymal transition process were all hindered in HCC cells. Significantly, circRNA-103809 competed with PLAG1 like zinc finger 2 (PLAGL2) for binding with microRNA-1270 (miR-1270), which formulated a competing endogenous RNA network in HCC. Thereafter, we verified the tumor-facilitating effect of circRNA-103809/miR-1270/PLAGL2 axis on biological behaviors of HCC cells.

Conclusion

Hsa-circRNA-103809 promoted development of HCC via sequestering miR-1270 and up-regulating PLAGL2.

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Acknowledgment

We appreciate all the participants who provided supports for this study.

Funding

None.

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Authors and Affiliations

  1. Department of General Surgery, Nanjing Drum Tower Hospital, No. 321 Zhongshan Road, Nanjing, 210000, Jiangsu, China

    Yajuan Cao & Xinhua Zhu

  2. Department of General Surgery, Zhongda Hospital, Clinical School of Southeast University, Nanjing, 210000, Jiangsu, China

    Qingsong Tao

  3. Department of General Surgery, Jinling Hospital, Nanjing Medical School of Nanjing University, Nanjing, 210000, Jiangsu, China

    Xiaoming Kao

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  1. Yajuan Cao
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  2. Qingsong Tao
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  4. Xinhua Zhu
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Correspondence to Xinhua Zhu.

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Supplementary Figure 1.

The expression levels of 12 candidate targets of miR-1270 were tested by qRT-PCR in HepG2 and Huh7 cells with or without boosted miR-1270 expression. All data were demonstrated as the mean ± SD. **P < 0.01, ns meant no significance (TIFF 652 kb)

Supplementary Figure 2.

A-E. The proliferation, migration and invasion of HepG2 and Huh7 cells with or without PLAGL2 up-regulation were determined by performing colony formation assay (A), CCK-8 assay (B), transwell assays (C-D) and wound healing assay (E). F. The level of E-cadherin and N-cadherin in above HCC cells was evaluated via western blot. All data were demonstrated as the mean ± SD. **P < 0.01 (TIFF 2726 kb)

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Cao, Y., Tao, Q., Kao, X. et al. Hsa-circRNA-103809 Promotes Hepatocellular Carcinoma Development via MicroRNA-1270/PLAG1 Like Zinc Finger 2 Axis. Dig Dis Sci 66, 1524–1532 (2021). https://doi.org/10.1007/s10620-020-06416-x

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  • DOI: https://doi.org/10.1007/s10620-020-06416-x

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