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LncRNA-CCAT1 Promotes Migration, Invasion, and EMT in Intrahepatic Cholangiocarcinoma Through Suppressing miR-152

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Abstract

Background

Increasing evidence has suggested that lncRNA CCAT1 is upregulated and functions as a potential tumor promoter in many cancers. However, the potential biological roles and regulatory mechanisms of CCAT1 in intrahepatic cholangiocarcinoma (ICC) remain unclear.

Methods

We used real-time PCR to measure CCAT1 expression in ICC tissues and the adjacent normal tissues. The statistical analyses were applied to evaluate the prognostic value and associations of CCAT1 expression with clinical parameters. The CCAT1 was silenced with siRNA in ICC cells. The migration and invasion of ICC cells were detected with Transwell assay. The expressions of epithelial–mesenchymal transition (EMT)-related proteins were evaluated to discover whether the process of EMT was involved.

Results

We found that CCAT1 expression was elevated in ICC tissues compared to the adjacent normal tissues. We also found that high CCAT1 expression is closely correlated with tumor progression in ICC patients. Furthermore, our results show that knockdown of CCAT1 significantly suppressed the migration and invasion of ICC cells. Additionally, CCAT1 silencing remarkably reverses the EMT phenotype of ICC cells. Moreover, bioinformatics analysis and luciferase reporter assay revealed that CCAT1 directly bound to the miR-152, which has been reported to serve as a tumor suppressor in variety cancers. Further investigation demonstrated that CCAT1 led to the metastasis and EMT activation of ICC cells through inhibiting miR-152.

Conclusions

Our results suggested that CCAT1 functions as an oncogenic lncRNA in ICC, which could serve as a potential diagnostic and therapeutic target for ICC patients.

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Acknowledgments

This study was supported by grants from the National Natural Science Foundation of China (No. 81660409)

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Authors and Affiliations

  1. Department of General Surgery, Jiangxi Children’s Hospital, Nanchang, 330006, Jiangxi Province, China

    Shouhua Zhang, Yong Chai & Zhiqiang Liu

  2. Department of Ultrasound, Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, 330006, Jiangxi Province, China

    Juhua Xiao & Xin Zhou

  3. Department of Otolaryngology, Jiangxi Provincial People’s Hospital, Nanchang, 330006, Jiangxi Province, China

    Yun yan Du

  4. Department of Gastrointestinal Surgery, Jiangxi Provincial Cancer Hospital, No. 519, Beijing East Road, Nanchang, 330029, Jiangxi Province, China

    Kai Huang & Wei Zhou

Authors
  1. Shouhua Zhang
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  2. Juhua Xiao
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  3. Yong Chai
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  4. Yun yan Du
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  5. Zhiqiang Liu
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  6. Kai Huang
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  7. Xin Zhou
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  8. Wei Zhou
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Correspondence to Wei Zhou.

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Zhang, S., Xiao, J., Chai, Y. et al. LncRNA-CCAT1 Promotes Migration, Invasion, and EMT in Intrahepatic Cholangiocarcinoma Through Suppressing miR-152. Dig Dis Sci 62, 3050–3058 (2017). https://doi.org/10.1007/s10620-017-4759-8

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  • DOI: https://doi.org/10.1007/s10620-017-4759-8

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