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MiR-3910 Promotes the Growth and Migration of Cancer Cells in the Progression of Hepatocellular Carcinoma

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Abstract

Introduction

Previous studies have reported that specific depletion of mammalian sterile-like kinase (MST1) in the mouse liver driven Hepatocellular carcinoma (HCC). However, how the expression of MST1 was regulated in the progression of HCC remains largely unknown.

Materials and methods

The expression of miR-3910 in the HCC tissues and cell lines were examined using q-PCR. The functions of miR-3910 in HCC were examined using MTT assay, Boyden chamber assay and soft agar assay. The effects of miR-3910 on the metastasis of HCC cells were evaluated using the mouse model.

Results

Here, we have shown that miR-3910 regulated the expression of MST1. MiR-3910 was up-regulated in HCC samples and cell lines, and the expression of miR-3910 was induced by the oncogenic RasV12. In the functional study, miR-3910 was found to promote the growth and migration of HCC cells, and knocking down miR-3910 inhibited the metastasis of HCC cells. Mechanically, it was found that miR-3910 activated YAP signaling by targeting MST1.

Conclusion

Taken together, this study demonstrated that miR-3910 exerted oncogenic effects on the progression of HCC and suggested that miR-3910 might be a therapeutic target for cancer therapy.

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Author notes

  1. Lina Cheng and Hongwei Wang contributed equally to this work.

Authors and Affiliations

  1. Department of Gastroenterology, People’s Hospital of Zhengzhou University (Henan Provincial People’s Hospital), 7th Weiwu Road, Zhengzhou, 450003, Henan Province, China

    Lina Cheng & Shuangyin Han

  2. Department of Anesthesiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China

    Hongwei Wang

Authors
  1. Lina Cheng
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  2. Hongwei Wang
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  3. Shuangyin Han
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Correspondence to Shuangyin Han.

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There is no conflict of interest.

Ethical approval research involving animals

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Research involving human participants

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

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Cheng, L., Wang, H. & Han, S. MiR-3910 Promotes the Growth and Migration of Cancer Cells in the Progression of Hepatocellular Carcinoma. Dig Dis Sci 62, 2812–2820 (2017). https://doi.org/10.1007/s10620-017-4670-3

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  • DOI: https://doi.org/10.1007/s10620-017-4670-3

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