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CHD1L Expression Increases Tumor Progression and Acts as a Predictive Biomarker for Poor Prognosis in Pancreatic Cancer

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Abstract

Background

The chromodomain helicase/ATPase DNA binding protein 1-like gene (CHD1L) plays a key role in controlling various cellular phenomena, including immune-mediated inflammation, transformation, apoptosis, cell cycle progression, and proliferation.

Methods

This study investigated the function and clinical significance of CHD1L protein expression in pancreatic cancer (PC). We analyzed CHD1L expression in surgical specimens from 112 PC patients. The correlation between the clinical characteristics and prognosis was also determined. Futhermore, cell proliferation were measured using EDU, and a molecular mechanism of Wnt/β-catenin pathway regulation by CHD1L was explored.

Result

CHD1L protein expression was significantly higher in PC patients with regard to the tumor grade, stage, size, differentiation and lymph node status. Increased CHD1L protein expression was significantly associated with poor overall survival. Multivariate analyses revealed that high CHD1L expression was an independent predictive marker for the recurrence and poor prognosis of pancreatic cancer. Furthermore, silencing of CHD1L expression by RNAi effectively abolished the proliferative abilities of CHD1L in vivo and in vitro. We found that the Wnt/β-catenin pathway contributed to the effect of CHD1L-mediated pancreatic cancer proliferation.

Conclusion

Taken together, our data provide a novel evidence for the biological and clinical significance of CHD1L as a potential biomarker, and we demonstrate that CHD1L-Wnt/β-catenin might be a novel pathway involved in pancreatic cancer progression.

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Acknowledgments

This study was supported by grants from the National Natural Science Foundation of China (No. 81200091). This work was supported by the Science and Technology Department of Jiangxi Province, China (No. 20143BBM26054).

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Author notes

  1. Chuan Liu and Xiaowei Fu have contributed equally to this work.

Authors and Affiliations

  1. Jiangxi Province Key Laboratory of Molecular Medicine, The Second Affiliated Hospital of Nanchang University, No. 1 Min De Road, Nanchang, 330006, China

    Chuan Liu, Haiyan Mou, Tianle Sheng & Yeqing Zou

  2. Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Nanchang University, No. 1 Min De Road, Nanchang, 330006, China

    Xiaowei Fu & Zhiwei Zhong

  3. Department of Clinical Laboratory, The Second Affiliated Hospital of Nanchang University, No. 1 Min De Road, Nanchang, 330006, China

    Jing Zhang, Qiong Wu & Bo Huang

Authors
  1. Chuan Liu
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  2. Xiaowei Fu
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  4. Jing Zhang
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Corresponding authors

Correspondence to Bo Huang or Yeqing Zou.

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Liu, C., Fu, X., Zhong, Z. et al. CHD1L Expression Increases Tumor Progression and Acts as a Predictive Biomarker for Poor Prognosis in Pancreatic Cancer. Dig Dis Sci 62, 2376–2385 (2017). https://doi.org/10.1007/s10620-017-4641-8

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  • DOI: https://doi.org/10.1007/s10620-017-4641-8

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