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Comparison of Adipose-Derived and Bone Marrow Mesenchymal Stromal Cells in a Murine Model of Crohn’s Disease

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Abstract

Background

Mesenchymal stromal cells (MSCs) have been used in the treatment of Crohn’s disease (CD) because of the immunomodulatory ability.

Aim

The aim of this study was to investigate the therapeutic effect of adipose-derived MSCs (AD-MSCs) and to compare the therapeutic effect of AD-MSCs with that of bone marrow MSCs (BM-MSCs) in a murine model of CD.

Methods

Murine colitis model of CD was created by trinitrobenzene sulfonic acid (TNBS). Twelve hours after treatment with TNBS, the mouse model was injected with MSCs intraperitoneally. Real-time polymerase chain reaction and immunohistochemistry staining were used to measure the expression levels of inflammatory cytokines in colonic tissues to investigate the therapeutic effect of AD-MSCs. The ten-day survival was recorded after infusion of MSCs.

Results

Intraperitoneal injection of MSCs alleviated the clinical and histopathologic severity of intestinal inflammation, and improved the survival of the TNBS-induced mouse model of CD. AD-MSCs could effectively increase the expression of interleukin-10 and reduce the secretion of pro-inflammatory cytokines including tumor necrosis factor-α, interleukin-12, and vascular endothelial growth factor. The mucosal injury was repaired by AD-MSCs. These effects were comparable between AD-MSCs and BM-MSCs.

Conclusions

The therapeutic effect appears similar between AD-MSCs and BM-MSCs in treating CD. AD-MSCs may be a potential alternative of cell-based therapy for CD.

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Acknowledgments

This study is supported by Guangdong Provincial Scientific Technology Foundation (#B2012149), National Natural Science Foundation of China (#81200332, #81300367, and #81570596), Pearl River S&T Nova Program of Guangzhou (#2014J2200040), Science and Technology Planning Project of Guangdong Province (#2015B020229001), and the Fundamental Research Funds for the Central Universities (#15ykjc06e).

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    Authors and Affiliations

    1. Department of Colorectal Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, 26 Yuancun Erheng Rd, Guangzhou, 510655, Guangdong, People’s Republic of China

      Minghao Xie, Huabo Qin, Qianxin Luo, Xiaosheng He, Xiaowen He, Ping Lan & Lei Lian

    2. Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, People’s Republic of China

      Minghao Xie, Huabo Qin, Qianxin Luo, Xiaosheng He, Xiaowen He, Ping Lan & Lei Lian

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    1. Minghao Xie
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    Correspondence to Lei Lian.

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    Minghao Xie and Huabo Qin have contributed equally to this work.

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    Xie, M., Qin, H., Luo, Q. et al. Comparison of Adipose-Derived and Bone Marrow Mesenchymal Stromal Cells in a Murine Model of Crohn’s Disease. Dig Dis Sci 62, 115–123 (2017). https://doi.org/10.1007/s10620-016-4166-6

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    • DOI: https://doi.org/10.1007/s10620-016-4166-6

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