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Upregulation of the Long Non-coding RNA PlncRNA-1 Promotes Esophageal Squamous Carcinoma Cell Proliferation and Correlates with Advanced Clinical Stage

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Abstract

Background

Recent studies revealed that long noncoding RNAs (lncRNAs) play critical regulatory roles in cancer biology. PlncRNA-1 is one of lncRNAs that is associated with cell apoptosis and proliferation of prostate cancer.

Aim

This study aimed to assess the potential role of PlncRNA-1 in the pathogenesis of esophageal squamous cell carcinoma (ESCC).

Materials and Methods

Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression level of PlncRNA-1 in 73 pairs of ESCC and their matched normal tissues. The correlation of PlncRNA-1 with clinicopathological features and clinical stages was also analyzed. Cancer cell proliferation and apoptosis were assessed following knock-down of PlncRNA-1 by MTT, colony formation assay, and flow cytometry.

Results

The expression of PlncRNA-1 was significantly higher in human ESCC compared with the adjacent noncancerous tissues (69.8 %, p < 0.05), and the high level of PlncRNA-1 expression was significantly correlated with advanced clinical stage (p < 0.01) and lymph node metastasis (p < 0.05). Furthermore, knockdown of PlncRNA-1 reduced cell proliferation and increased the apoptosis in vitro.

Conclusions

PlncRNA-1 plays an important role in ESCC cell proliferation. Overexpression of PlncRNA-1 is correlated with advanced tumor stage and lymph node metastasis, and may serve as a potential prognostic marker and therapeutic target for ESCC.

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References

  1. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin. 2011;61:69–90.

    Article  PubMed  Google Scholar 

  2. Matsushima K, Isomoto H, Yamaguchi N, et al. MiRNA-205 modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox 2 expression in esophageal squamous cell carcinoma cells. J Transl Med. 2011;9:30. doi:10.1186/1479-5876-9-30.

    Google Scholar 

  3. Feber A, Xi L, Luketich JD, et al. MicroRNA expression profiles of esophageal cancer. J Thorac Cardiovasc Surg. 2008;135:255–260.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Ji X, Takahashi R, Hiura Y, Hirokawa G, Fukushima Y, Iwai N. Plasma miR-208 as a biomarker of myocardial injury. Clin Chem. 2009;55:1944–1949.

    Article  CAS  PubMed  Google Scholar 

  5. Barber TW, Duong CP, Leong T, Bressel M, Drummond EG, Hicks RJ. 18F-FDG PET/CT has a high impact on patient management and provides powerful prognostic stratification in the primary staging of esophageal cancer: a prospective study with mature survival data. J Nucl Med. 2012;53:864–871.

    Article  PubMed  Google Scholar 

  6. Sharma S, Kelly TK, Jones PA. Epigenetics in cancer. Carcinogenesis. 2010;31:27–36.

    Article  CAS  PubMed  Google Scholar 

  7. Mohammad F, Mondal T, Guseva N, Pandey GK, Kanduri C. Kcnq1ot1 noncoding RNA mediates transcriptional gene silencing by interacting with Dnmt1. Development. 2010;137:2493–2499.

    Article  CAS  PubMed  Google Scholar 

  8. Tsai MC, Manor O, Wan Y, et al. Long noncoding RNA as modular scaffold of histone modification complexes. Science. 2010;329:689–693.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  9. Gupta RA, Shah N, Wang KC, et al. Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature. 2010;464:1071–1076.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  10. Wang KC, Yang YW, Liu B, et al. A long noncoding RNA maintains active chromatin to coordinate homeotic gene expression. Nature. 2011;472:120–126.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  11. Khalil AM, Guttman M, Huarte M, et al. Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression. Proc Natl Acad Sci USA. 2009;106:11667–11672.

    Article  CAS  PubMed  Google Scholar 

  12. Carninci P, Kasukawa T, Katayama S. The transcriptional landscape of the mammalian genome. Science. 2005;309:1559–1563.

    Article  CAS  PubMed  Google Scholar 

  13. Augoff K, McCue B, Plow EF, Sossey-Alaoui K. miR-31 and its host gene lncRNA LOC554202 are regulated by promoter hypermethylation in triple-negative breast cancer. Mol Cancer. 2012;11:5. doi:10.1186/1476-4598-11-5.

  14. Liu Q, Huang J, Zhou N, et al. LncRNA loc285194 is a p53-regulated tumor suppressor. Nucleic Acids Res. 2013;41:4976–4987. doi:10.1093/nar/gkt182.

    Google Scholar 

  15. Flockhart RJ, Webster DE, Qu K, et al. BRAFV600E remodels the melanocyte transcriptome and induces BANCR to regulate melanoma cell migration. Genome Res. 2012;22:1006–1014.

    Article  CAS  PubMed  Google Scholar 

  16. Yang F, Huo XS, Yuan SX, et al. Repression of the long noncoding RNA-LET by histone deacetylase 3 contributes to hypoxia-mediated metastasis. Mol Cell. 2013;49:1083–1096.

    Article  CAS  PubMed  Google Scholar 

  17. Gutschner T, Hammerle M, Eissmann M, et al. The noncoding RNA MALAT1 is a critical regulator of the metastasis phenotype of lung cancer cells. Cancer Res. 2013;73:1180–1189.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  18. Tripathi V, Shen Z, Chakraborty A, et al. Long noncoding RNA MALAT1 controls cell cycle progression by regulating the expression of oncogenic transcription factor B-MYB. PLoS Genet. 2013;9:e1003368.

    Google Scholar 

  19. Kogo R, Shimamura T, Mimori K, et al. Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers. Cancer Res. 2011;71:6320–6326.

    Article  CAS  PubMed  Google Scholar 

  20. Aguilo F, Zhou MM, Walsh MJ. Long noncoding RNA, polycomb, and the ghosts haunting INK4b-ARF-INK4a expression. Cancer Res. 2011;71:5365–5369.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  21. Cui Z, Ren S, Lu J, et al. The prostate cancer-up-regulated long noncoding RNA PlncRNA-1 modulates apoptosis and proliferation through reciprocal regulation of androgen receptor. Urol Onco. 2012;31:1117–1123. doi:10.1016/j.urolonc.2011.11.030.

    Google Scholar 

  22. Kanda Y, Nishiyama Y, Shimada Y, et al. Analysis of gene amplification and overexpression in human esophageal-carcinoma cell lines. Int J Cancer. 1994;58:291–297.

    Article  CAS  PubMed  Google Scholar 

  23. Shimada Y, Imamura M, Wagata T, Yamaguchi N, Tobe T. Characterization of 21 newly established esophageal cancer cell lines. Cancer. 1992;69:277–284.

    Article  CAS  PubMed  Google Scholar 

  24. Yu J, Chen KS, Li YN, Yang J, Zhao L. Silencing of PDK1 gene expression by RNA interference suppresses growth of esophageal cancer. Asian Pac J Cancer Prev. 2012;13:4147–4151.

    Article  PubMed  Google Scholar 

  25. Tian Y, Luo A, Cai Y, et al. MicroRNA-10b promotes migration and invasion through KLF4 in human esophageal cancer cell lines. J Biol Chem. 2010;285:7986–7994.

    Article  CAS  PubMed  Google Scholar 

  26. Szymanski M, Barciszewski J. Beyond the proteome: non-coding regulatory RNAs. Genome Biol. 2002;3:5(0005.1–0005.8).

  27. Chen F-J, Sun M, Li S-Q, et al. Upregulation of the long non-coding RNA HOTAIR promotes esophageal squamous cell carcinoma metastasis and poor prognosis. Mol Carcinog. 2013;52:908–915. doi:10.1002/mc.21944.

    Google Scholar 

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Acknowledgments

This work was supported by the Graduate Research and Innovation Projects of Jiangsu Province (Grant No. CXLX_0567), the Foundation of Nanjing City Committee of Science and Technology (Grant No. 201108027), the Innovation Team of Science and Education Health Project of Jiangsu Province in 2011 (Grant No. Jiangsu Science and Education Health Project 2011 no.15) and the National Natural Science Foundation of China (Grant No. H1617/81201881).

Conflict of interest

None.

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Authors and Affiliations

  1. Department of Surgical Oncology, Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

    Chun-Mei Wang, Su-Qing Li, Lei Tuo, Hai-Wei Xie, Yu-Suo Tong, Lv Ji, Guo-Zhi Zhou, Gang Cao, Jin Lv & Xiu-Feng Cao

  2. Department of Oncology, Changzhou Second Hospital Affiliated to Nanjing Medical University, Changzhou, Jiangsu, China

    Fang-Jun Chen

  3. Huaian First Hospital, Nanjing Medical University, Huaian, Jiangsu, China

    Qing-Quan Wu

  4. Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu, China

    Ming Wu

  5. Department of Medical Technology, Yancheng Health Vocational, Yancheng, Jiangsu, China

    Wei-Hong Shi

Authors
  1. Chun-Mei Wang
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  2. Qing-Quan Wu
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  3. Su-Qing Li
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  4. Fang-Jun Chen
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  5. Lei Tuo
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  6. Hai-Wei Xie
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  7. Yu-Suo Tong
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  8. Lv Ji
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  9. Guo-Zhi Zhou
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  10. Gang Cao
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  11. Ming Wu
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  12. Jin Lv
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  13. Wei-Hong Shi
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  14. Xiu-Feng Cao
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Corresponding authors

Correspondence to Jin Lv, Wei-Hong Shi or Xiu-Feng Cao.

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Wang, CM., Wu, QQ., Li, SQ. et al. Upregulation of the Long Non-coding RNA PlncRNA-1 Promotes Esophageal Squamous Carcinoma Cell Proliferation and Correlates with Advanced Clinical Stage. Dig Dis Sci 59, 591–597 (2014). https://doi.org/10.1007/s10620-013-2956-7

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  • DOI: https://doi.org/10.1007/s10620-013-2956-7

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