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Ileal Mucosal and Fecal Pancreatitis Associated Protein Levels Reflect Severity of Salmonella Infection in Rats

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Abstract

Background Microbial infections induce ileal pancreatitis-associated protein/regenerating gene III (PAP/RegIII) mRNA expression. Despite increasing interest, little is known about the PAP/RegIII protein. Therefore, ileal mucosal PAP/RegIII protein expression, localization, and fecal excretion were studied in rats upon Salmonella infection. Results Salmonella infection increased ileal mucosal PAP/RegIII protein levels in enterocytes located at the crypt-villus junction. Increased colonization and translocation of Salmonella was associated with higher ileal mucosal PAP/RegIII levels and secretion of this protein in feces. Conclusions PAP/RegIII protein is increased in enterocytes of the ileal mucosa during Salmonella infection and is associated with infection severity. PAP/RegIII is excreted in feces and might be used as a new and non-invasive infection marker.

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Acknowledgments

We would like to thank the biotechnicians at the Small Animal Centre of Wageningen University (Wageningen, The Netherlands) for excellent assistance. We also would like to thank Prof. J. H. van Krieken at the Department of Pathology, University Medical Centre Nijmegen, for his advice on histology results, our colleagues at the Department of Health and Safety of NIZO food research, and Prof. Robert-Jan Brummer of TI Food and Nutrition for fruitful discussions. This study was funded by TI Food and Nutrition.

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Authors and Affiliations

  1. TI Food and Nutrition, Wageningen, The Netherlands

    Marleen T. J. van Ampting, Wendy Rodenburg, Carolien Vink, Evelien Kramer, Arjan J. Schonewille, Jaap Keijer, Roelof van der Meer & Ingeborg M. J. Bovee-Oudenhoven

  2. Department of Health and Safety, NIZO Food Research, P.O. Box 20, 6710 BA, Ede, The Netherlands

    Marleen T. J. van Ampting, Wendy Rodenburg, Carolien Vink, Arjan J. Schonewille, Roelof van der Meer & Ingeborg M. J. Bovee-Oudenhoven

  3. RIKILT Institute of Food Safety, Wageningen, The Netherlands

    Wendy Rodenburg, Evelien Kramer & Jaap Keijer

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  1. Marleen T. J. van Ampting
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Correspondence to Ingeborg M. J. Bovee-Oudenhoven.

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van Ampting, M.T.J., Rodenburg, W., Vink, C. et al. Ileal Mucosal and Fecal Pancreatitis Associated Protein Levels Reflect Severity of Salmonella Infection in Rats. Dig Dis Sci 54, 2588–2597 (2009). https://doi.org/10.1007/s10620-008-0685-0

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  • DOI: https://doi.org/10.1007/s10620-008-0685-0

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