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Midkine mRNA Is Overexpressed in Pancreatic Cancer

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Abstract

Purpose Midkine (MK) has been reported to be a possible molecular marker for the diagnosis of pancreatic cancer. We investigated the feasibility of quantitative analysis of MK mRNA by quantitative real-time RT-PCR (qRT-PCR) as a promising tool for the diagnosis of pancreatic cancer. Results We found that pancreatic cancer tissues expressed significantly higher levels of MK mRNA than intraductal pancreatic mucinous neoplasm (IPMN) and non-neoplastic pancreatic tissues (P < 0.05); in contrast, we did not find any differences in MK mRNA expression between IPMN and non-neoplastic pancreatic tissues. Additionally, we observed that poorly differentiated carcinoma samples expressed higher levels of MK mRNA than well-differentiated carcinoma samples, although a significant difference was not observed. Conclusions The present data suggests that quantitative analysis of MK mRNA provides an objective and sensitive evaluation and may be a promising modality for the diagnosis of pancreatic cancer and the prediction of its prognosis.

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Acknowledgments

This research work was supported in part by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and grants from the Fukuoka Cancer Society and Kaibara Morikazu Medical Science Promotion Foundation. We are grateful to Emiko Manabe, Makiko Masuda, and Miyuki Omori (Department of Surgery and Oncology, Kyushu University) for skillful technical assistance.

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Authors and Affiliations

  1. Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Fukuoka, 812-8582, Japan

    Seiji Ohhashi, Kenoki Ohuchida, Kazuhiro Mizumoto, Takuya Egami, Jun Yu, Lin Cui, Hiroki Toma, Shunichi Takahata, Toshinaga Nabae & Masao Tanaka

  2. Department of Advanced Medical Initiatives, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

    Kenoki Ohuchida

  3. Kyushu University Hospital Cancer Center, Fukuoka, Japan

    Kazuhiro Mizumoto

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  1. Seiji Ohhashi
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  2. Kenoki Ohuchida
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  3. Kazuhiro Mizumoto
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  4. Takuya Egami
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  5. Jun Yu
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  6. Lin Cui
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  7. Hiroki Toma
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  9. Toshinaga Nabae
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  10. Masao Tanaka
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Correspondence to Kenoki Ohuchida.

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Ohhashi, S., Ohuchida, K., Mizumoto, K. et al. Midkine mRNA Is Overexpressed in Pancreatic Cancer. Dig Dis Sci 54, 811–815 (2009). https://doi.org/10.1007/s10620-008-0434-4

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  • DOI: https://doi.org/10.1007/s10620-008-0434-4

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