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Fasting Exacerbates and Feeding Diminishes LPS-Induced Liver Injury in the Rat

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Introduction Enteral nutrition improves clinical outcomes. The effects of feeding on LPS induced liver injury are unknown. We hypothesized that feeding would attenuate liver injury from LPS. Methods Fasted or fed rats were given LPS (20 mg/kg ip) or saline for 5 h and sacrificed. Serum aminotransferases and cytokines (immunoassay) were measured. Oxidative stress protein (iNOS, COX2, and HO1) assessments (Western immunoblot) were also obtained. Results In fasted rats, LPS significantly increased serum aminotransferase levels, enhanced hepatic COX2, iNOS, and HO1 immunoreactivity, and increased serum cytokine levels when compared to controls. While feeding diminished liver enzymes, attenuated expression of COX2 and iNOS, and blunted production of pro-inflammatory cytokines, it did not modulate LPS-induced expression of the anti-inflammatory markers HO1 and IL-10. Conclusion These data suggest that feeding decreases liver injury by attenuating expression of pro-inflammatory mediators while maintaining expression of anti-inflammatory mediators, both systemically and locally.

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Acknowledgments

The authors graciously thank Lily Chang, BS, Yan Cui, MD, and Raymond Kwan, BS, for their technical assistance in carrying out these experiments. This research was supported by grants from NIGMS GM-38529 and GM-08792.

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  1. Department of Surgery, University of Texas Health Science Center, 6431 Fannin Street, Suite 4.264, Houston, TX, 77030, USA

    Sasha D. Adams, Benjamin A. Delano, Kenneth S. Helmer & David W. Mercer

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  1. Sasha D. Adams
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Correspondence to David W. Mercer.

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Adams, S.D., Delano, B.A., Helmer, K.S. et al. Fasting Exacerbates and Feeding Diminishes LPS-Induced Liver Injury in the Rat. Dig Dis Sci 54, 767–773 (2009). https://doi.org/10.1007/s10620-008-0425-5

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