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The Effects of Capsaicin on Gastrin Secretion in Isolated Human Antral Glands: Before and After Ingestion of Red Chilli

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Abstract

Background Capsaicin is known to have regulatory effects on gastrointestinal functions via the vanilloid receptor (VR1). We reported previously that endocrine-like cells in the human antrum express VR1. Aim To identify VR1-expressing endocrine-like cells in human antral glands and to examine whether stimulation with capsaicin causes release of gastrin, somatostatin, and serotonin. Further, to investigate the effects of a chilli-rich diet. Methods Gastroscopic biopsies were received from 11 volunteers. Seven of the 11 subjects agreed to donor gastric biopsies a second time after a 3-week chilli-rich diet containing 1.4–4.2 mg capsaicin/day. VR1-immunoreactive cells were identified by double-staining immunohistochemistry against gastrin, somatostatin, and serotonin. For the stimulation studies, we used an in vitro method where antral glands in suspension were stimulated with 0.01 mM capsaicin and physiological buffer was added to the control vials. The concentrations of secreted hormones were detected and calculated with radioimmunoassay (RIA). Results The light microscopic examination revealed that VR1 was localized in gastrin cells. The secretory studies showed an increase in release of gastrin and somatostatin compared to the control vials (P = 0.003; P = 0.013). Capsaicin-stimulation caused a consistent raise of the gastrin concentrations in the gland preparations from all subjects. A chilli-rich diet had an inhibitory effect on gastrin release upon stimulation compared to the results that were obtained before the start of the diet. Conclusion This study shows that capsaicin stimulates gastrin secretion from isolated human antral glands, and that a chilli-rich diet decreases this secretion.

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Acknowledgments

The authors would like to thank Inga-Lill Andersson and Marja Tjädermo for excellent and skillful assistance. This work is supported by grants from Forskningsrådet i sydöstra Sverige (FORSS).

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Authors and Affiliations

  1. Department of Experimental and Clinical Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden

    A. Ericson & E. Mohammed Nur

  2. Department of Pathology, Karolinska University Hospital, Stockholm, Sweden

    F. Petersson

  3. Division of Internal Medicine, Department of Medical and Health Sciences, University Hospital, Linköping, Sweden

    S. Kechagias

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  1. A. Ericson
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  2. E. Mohammed Nur
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  3. F. Petersson
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  4. S. Kechagias
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Correspondence to A. Ericson.

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Ericson, A., Nur, E.M., Petersson, F. et al. The Effects of Capsaicin on Gastrin Secretion in Isolated Human Antral Glands: Before and After Ingestion of Red Chilli. Dig Dis Sci 54, 491–498 (2009). https://doi.org/10.1007/s10620-008-0400-1

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  • DOI: https://doi.org/10.1007/s10620-008-0400-1

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