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Estimation of the Potential Antitumor Activity of Microencapsulated Lactobacillus acidophilus Yogurt Formulation in the Attenuation of Tumorigenesis in Apc(Min/+) Mice

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Abstract

There is a strong correlation between orally administered probiotics and suppression of the low-grade inflammation that can lead to restoration of normal local immune functions. We studied the potential immunomodulatory and antitumorigenic properties of microencapsulated probiotic bacterial cells in a yogurt formulation in Min mice carrying a germline APC mutation. Daily oral administration of microencapsulated Lactobacillus acidophilus bacterial cells in the yogurt formulation mice resulted in significant suppression of colon tumor incidence, tumor multiplicity, and reduced tumor size. Results show that oral administration of microencapsulated L. acidophilus contributed to the stabilization of animal body weight and decreased the release of bile acids. Histopathological analyses revealed fewer adenomas in treated versus untreated animals. Furthermore, treated animals exhibited fewer gastrointestinal intra-epithelial neoplasias with a lower grade of dysplasia in detected tumors. Results suggest that oral administration of microencapsulated probiotic L. acidophilus exerts anti-tumorous activity, which consequently leads to reduced tumor outcome.

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Acknowledgments

We acknowledge the Canadian Institute of Health Research (CIHR) and Micropharma grants to Dr. S. Prakash. We also acknowledge support for A.M. Urbanska from a PGS-D scholarship from the Natural Sciences and Engineering Research Council (NSERC) of Canada and a McGill Faculty of Medicine Internal postgraduate scholarship. J. Bhathena acknowledges the Canadian Liver Foundation for a Graduate Studentship Award. C. Martoni and A. Kulamarva acknowledge graduate scholarships (doctoral) from NSERC. We acknowledge the aid of Dr. Marilene Paquet, Veterinary Pathologist, McGill Cancer Center, in performing the blinded scoring for the histological experiments as well as Arun Kulamarva for help with fecal sample collection. This work was supported by CIHR.

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Authors and Affiliations

  1. Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, QC, Canada, H3A 2B4

    Aleksandra Malgorzata Urbanska, Jasmine Bhathena, Christopher Martoni & Satya Prakash

  2. Department of Physiology, Faculty of Medicine, McGill University, 3775 University Street, Montreal, QC, Canada, H3A 2B4

    Aleksandra Malgorzata Urbanska, Jasmine Bhathena, Christopher Martoni & Satya Prakash

  3. Artificial Cells and Organ Research Center, Faculty of Medicine, McGill University, 3775 University Street, Montreal, QC, Canada, H3A 2B4

    Aleksandra Malgorzata Urbanska, Jasmine Bhathena, Christopher Martoni & Satya Prakash

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  1. Aleksandra Malgorzata Urbanska
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  2. Jasmine Bhathena
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Correspondence to Satya Prakash.

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Urbanska, A.M., Bhathena, J., Martoni, C. et al. Estimation of the Potential Antitumor Activity of Microencapsulated Lactobacillus acidophilus Yogurt Formulation in the Attenuation of Tumorigenesis in Apc(Min/+) Mice. Dig Dis Sci 54, 264–273 (2009). https://doi.org/10.1007/s10620-008-0363-2

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