Skip to main content
SpringerLink
Log in

Omeprazole Inhibits Natural Killer Cell Functions

  • Original Paper
  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

This study was designed to determine the possible effects of omeprazole on human natural killer cells. Peripheral venous blood samples were taken from 20 peptic ulcer patients before and at the 14th and the 28th days of omeprazole treatment. Mononuclear cells were removed from blood and their capability of making conjugation with K562 target cells and lysing K562 target cells was evaluated. A significant decrease was found (P < 0.001) in the 14th and the 28th days compared with the basal value of the capability of the mononuclear cells to conjugate with the K562 target cells and to lyse them. This study demonstrated that omeprazole significantly reduces natural killer cell functions. This finding suggests that omeprazole may also have some effects on the other systems in addition to parietal cell acid secretion.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Clissold SP, Campoli-Richards DM (1986) Omeprazole: a preliminary review of its pharmacodynamic and pharmacokinetic properties and therapeutic potential in peptic ulcer disease and Zollinger-Ellison Syndrome. Drugs 32:15–47

    Article  PubMed  CAS  Google Scholar 

  2. Fellenius E, Berglindh T, Sachs G, Olbe L, Elander B, Sjostrand SE, Wallmark B (1981) Substituted benzimidazoles inhibit gastric acid secretion by blocking (H+–K+) ATPase. Nature 290:159–161

    Article  PubMed  CAS  Google Scholar 

  3. Fryklund I, Gedda K, Wallmark B (1988) Specific labelling of gastric H+, K+ ATPase by omeprazole. Biochem Pharmacol 37:2543–2549

    Article  PubMed  CAS  Google Scholar 

  4. Keeling DJ, Fallowfield C, Underwood AH (1987) The specificity of omeprazole as an (H+–K+)-ATPase inhibitor depends upon the means of its activation. Biochem Pharmacol 36:339–344

    Article  PubMed  CAS  Google Scholar 

  5. Sachs G, Wallmark B (1989) The gastric H+,K+-ATPase: the site of action of omeprazole. Scand J Gastroenterol 24(Suppl 166):3–11

    Article  Google Scholar 

  6. Amzel LM, Pedersen PL (1983) Proton ATPases: structure and mechanism. Ann Rev Biochem 52:801–824

    Article  PubMed  CAS  Google Scholar 

  7. Anderson RGW, Orci L (1988) A view of acidic intracellular compartments. J Cell Biol 106:539–545

    Article  PubMed  CAS  Google Scholar 

  8. Mellman I, Fuchs R, Helenius A (1986) Acidification of the endocytic and exocytic pathways. Ann Rev Biochem 55:663–700

    Article  PubMed  CAS  Google Scholar 

  9. Wandall JH (1992) Effects of omeprazole on neutrophil chemotaxis, super oxide production, degranulation and translocation of cytochrome b-245. Gut 33:617–621

    Article  PubMed  CAS  Google Scholar 

  10. Aybay C, Imir T, Okur H (1995) The effect of omeprazole on human natural killer cell activity. Gen Pharmacol 26:1413–1418

    PubMed  CAS  Google Scholar 

  11. Timonen T, Ortaldo JR, Herberman RB (1981) Characteristics of human large granular lymphocytes and relationship to natural killer and K cells. J Exp Med 153:569–582

    Article  PubMed  CAS  Google Scholar 

  12. Imir T, Sibbitt W, Bankhurst A (1987) The relative resistance of lymphokine activated killer cells to suppression by prostaglandins and glucocorticoids. Prostaglandins Leukotriens Med 28:111–118

    Article  CAS  Google Scholar 

  13. Bankhurst AD, Imir T (1989) The mechanism involved in the activation of human natural killer cells by Staphylococcal Enterotoxin B. Cell Immunol 122:108–121

    Article  PubMed  Google Scholar 

  14. Ullberg M, Jondal M (1981) Recycling and target binding capacity of human natural killer cells. J Exp Med 153:615–628

    Article  PubMed  CAS  Google Scholar 

  15. Scaringi L, Cornacchione P, Fettucciari K, Rosati E, Rossi R, Marconi P, Capadicosa E (1996) Activity inhibition of cytolytic lymphocytes by omeprazole. Scand J Immunol 44:204–214

    Article  PubMed  CAS  Google Scholar 

  16. Capodicasa E, De Bellis F, Pelli MA (1999) Effect of lansoprazole on human leukocyte function. Immunopharmacol Immunotoxicol 21:357–377

    Article  PubMed  CAS  Google Scholar 

  17. Shamburek RD, Ruddy S, Schubert ML (1993) Omeprazole and neutrophil function. Gastroenterology 104:938–940

    PubMed  CAS  Google Scholar 

  18. Mc Tavish D, Buckley MM-T, Heel RC (1991) Omeprazole, an updated review of its pharmacology and therapeutic use in acid-related disorders. Drugs 42:138–170

    CAS  Google Scholar 

  19. Lindberg P, Nordberg P, Alminger T, Brandstrom A, Wallmark B (1986) The mechanism of action of the gastric acid secretion inhibitor omeprazole. J Med Chem 29:1327–1329

    Article  PubMed  CAS  Google Scholar 

  20. Verhoef J, Sharma SD (1983) Inhibition of human natural killer activity by lysosomotropic agents. J Immunol 131:125–131

    PubMed  CAS  Google Scholar 

  21. De Duwe C (1983) Lysosomes revisited. Eur J Biochem 137:391–397

    Article  Google Scholar 

  22. Deem RL, Targan SR (1984) Evidence of a dynamic role of the target cell membrane during the early stages of the Natural Killer cell lethal hit. J Immunol 133:72–77

    PubMed  CAS  Google Scholar 

  23. Suzuki M, Mori M, Miura S, Suematsu M, Fukumura D, Kimura H, Ishii H (1996) Omeprazole attenuates oxygen-derived free radical production from human neutrophils. Free Radic Biol Med 21:727–731

    Article  PubMed  CAS  Google Scholar 

  24. Suzuki M, Nakamura M, Mori M, Miura S, Tsuchiya M, Ishii H (1995) Lansoprazole inhibits oxygen-derived free radical production from neutrophils activated by Helicobacter pylori. J Clin Gastroenterol 20(Suppl 2):S93–S96

    Article  PubMed  Google Scholar 

  25. Suzuki M, Mori M, Fukumura D, Suzuki H, Miura S, Ishii H (1999) Omeprazole attenuates neutrophil-endothelial cell adhesive interaction induced by extracts of Helicobacter pylori. J Gastroenterol Hepatol 14:27–31

    Article  PubMed  CAS  Google Scholar 

  26. Suzuki H, Miyazawa M, Nagahashi S, Sato M, Bessho M, Nagata H, Miura S, Ishii H (2003) Rabeprazole treatment attenuated Helicobacter pylori-associated gastric mucosal lesion formation in Mongolian gerbils. J Gastroenterol Hepatol 18:787–795

    Article  PubMed  CAS  Google Scholar 

  27. Yoshida N, Yoshikawa T, Tanaka Y, Fujita N, Kassai K, Naito Y, Kondo M (2000) A new mechanism for anti-inflammatory actions of proton pump inhibitors – inhibitory effects on neutrophil-endothelial cell interactions. Aliment Pharmacol Ther 14(Suppl 1):74–81

    Article  PubMed  CAS  Google Scholar 

  28. Valori RM, Cockal R (1990) Omeprazole for duodenal ulceration in Crohn’s Disease. Br Med J 300:438–439

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Gastroenterology, Faculty of Medicine, Gazi University, Ankara, Turkey

    Huseyin Alkim & Selahattin Unal

  2. Nisantas Ihlamir Yolu, Ihlamur Yolu Çikmazi, Unsal Karahan Sitesi, C Blok, D: 49, Tesvikiye, Sisli, Istanbul, 34365, Turkey

    Huseyin Alkim

  3. Department of Immunology, Faculty of Medicine, Gazi University, Ankara, Turkey

    Hamza Okur & Turgut Imir

Authors
  1. Huseyin Alkim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Selahattin Unal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hamza Okur
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Turgut Imir
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Huseyin Alkim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Alkim, H., Unal, S., Okur, H. et al. Omeprazole Inhibits Natural Killer Cell Functions. Dig Dis Sci 53, 347–351 (2008). https://doi.org/10.1007/s10620-007-9869-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10620-007-9869-2

Keywords

Search

Navigation