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Helicobacter Pylori Infection Induces Candidate Stem Cell Marker Musashi-1 in the Human Gastric Epithelium

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Abstract

Musashi-1 (Msi-1), a mammalian neural RNA-binding protein, has been found to play important roles in the maintenance of stem cell states and differentiation in neural stem cells and mouse intestinal cells. We explored Msi-1 expression and its potential implications in the human stomach. Reverse transcription-PCR revealed that Msi-1 levels were significantly higher in the corpus than in antrum in Helicobacter pylori (Hp)-infected patients (n = 49) (P < 0.00001) in paired biopsy samples, whereas they were low and comparable at these two sites in Hp-negative patients (n = 31). Msi-1 levels were significantly higher in the Hp-infected corpus (n = 107) than in the Hp-negative corpus (n = 69) (P < 0.00000001). Immunohistochemistry and in situ hybridization demonstrated that Msi-1 was expressed at the base and neck/isthmus region of the fundic glands and partly co-expressed in Ki-67-positive cells in the corpus and antrum. Msi-1 levels correlated with Hp density (P < 0.05). Based on these results, we conclude that Hp infection strongly induces Msi-1 in the corpus. Given its expression in dividing cells, Msi-1 may modulate the state of gastric progenitor cells.

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Acknowledgements

This study was in part supported by the grants in aid from Japan Society for Promotion of Sciences.

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Authors and Affiliations

  1. Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, 2-2, Yamada-Oka, Suita, Osaka, 565-0871, Japan

    Hiroaki Murata, Shingo Tsuji, Masahiko Tsujii & Norio Hayashi

  2. Department of Clinical Laboratory Science, School of Allied Health Sciences, Osaka University Graduate School, Osaka, Japan

    Hiroaki Murata, Toru Nakamura, Hai Ying Fu, Hiroshi Eguchi, Kayoko Asahi & Sunao Kawano

  3. Department of Physiology, Keio University School of Medicine, Tokyo, Japan

    Hideyuki Okano

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  1. Hiroaki Murata
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  2. Shingo Tsuji
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  10. Norio Hayashi
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Correspondence to Hiroaki Murata.

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Murata, H., Tsuji, S., Tsujii, M. et al. Helicobacter Pylori Infection Induces Candidate Stem Cell Marker Musashi-1 in the Human Gastric Epithelium. Dig Dis Sci 53, 363–369 (2008). https://doi.org/10.1007/s10620-007-9858-5

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  • DOI: https://doi.org/10.1007/s10620-007-9858-5

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