Abstract
The major heat shock protein, HSP70, is known to be involved in cytoprotection against environmental stresses mediated by their function as a “molecular chaperone.” However, the influence of HSP70 on gastric mucosal healing under physical stimulation or stress is not completely understood. Rat gastric mucosal cells (RGM-1) were stably transfected with pBK-CMV containing the human HSP70 gene (7018-RGM-1) or pBK-CMV alone (pBK-CMV-12). Artificial wounds were created. Mechanical stretch was applied to 7018-RGM-1 cells or pBK-CMV-12 cells. The effect of mechanical stretch on HSP70 expression was assessed by Western blot analysis. Expression of HSP70 was decreased by mechanical stretch in pBK-CMV-12 cells. However, expression of HSP70 was not decreased by mechanical stretch in 7018-RGM-1 cells. Furthermore, the wound restoration of pBK-CMV-12 cells was suppressed under mechanical stretch condition. On the other hand, the wound restoration of 7018-RGM-1 cells was not affected by mechanical stretch. These results suggest that HSP70 plays an important role in gastric wound healing under physical stress.
Similar content being viewed by others
References
Hightower LE (1980) Cultured animal cells exposed to amino acid analogues or puromycin rapidly synthesize several polypeptides. J Cell Physiol 102:407–427
Itoh H, Tashima Y (1991) The stress (heat shock) proteins. Int J Biochem 23:1185–1191
Tissieres A, Mitchell HK, Tracy VM (1974) Protein synthesis in salivary glands of Drosophila melanogaster: relation to chromosome puffs. J Mol Biol 84:389–393
Nakamura K, Rokutan K, Narui N, Aoike A, Kawai K (1991) Induction of heat shock proteins and their implication in protection against ethanol-induced damage in cultured guinea pig gastric mucosal cells. Gastroenterology 101:161–166
Shoeniger LO, Andreoni KA, Ott GR, Risby TH, Bulkley GB, Udelsman R, Burdick JF, Buchman TG (1994) Induction of heat- shock gene expression in postischemic pig liver depends on superoxide generation. Gastroenterology 106:177–184
Otaka M, Okuyama A, Otani S, Jin M, Itoh S, Itoh H, Tashima Y, Masamune O (1997) Differential induction of HSP60 and HSP72 by different stress situations in rats—correlation with cerulein-induced pancreatitis. Dig Dis Sci 42:1473–1479
Odashima M, Otaka M, Jin M, Konishi N, Sato T, Kato S, Matsuhashi T, Nakamura C, Watanabe S (2003) Induction of a 72-kDa heat shock protein in cultured rat gastric mucosal cells and rat gastric mucosa by zinc L-carnosine. Dig Dis Sci 47:2799–2804
Tsukimi Y, Nakai H, Itoh S, Amagase K, Okabe S (2001) Involvement of heat shock proteins in the healing of acetic acid-induced gastric ulcers in rats. J Physiol Pharmacol 52:391–406
Osada T, Watanabe S, Hirose M, Sato N (1999) Effect of mechanical strain on gastric cellular migration and proliferation during mucosal healing: role of Rho dependent and Rac dependent cytoskeletal reorganization. Gut 45:508–515
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–225
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
Watanabe S, Wang XE, Hirose M, Oide H, Kitamura T, Miwa H, Miyazaki A, Sato N (1996) Platelet-derived growth factor accelerates gastric epithelial restoration in a rabbit cultured cell model. Gastroenterology 110:775–779
Oyake J, Otaka M, Matsuhashi T, Jin M, Odashima M, Komatsu K, Horikawa Y, Ohba R, Hatakeyama N, Itoh H, Watanabe S (2006) Over-expression of 70-kDa heat shock protein confers protection against monochloramine-induced gastric mucosal cell injury. Life Sci 79:300–305
Otaka M, Matsuhashi T, Odashima M, Itoh H, Jin M, Wada I, Komatsu K, Horikawa Y, Ohba R, Oyake J, Hatakeyama N, Watanabe S (2006) Enhancement of cytoprotective ability and cell restoration in 70-kDa heat shock protein gene-transfected rat gastric mucosal cells. Aliment Pharmacol Ther (Symp Ser) 2:272–277
Kovalchin JT, Wang R, Wagh MS, Azoulay J, Sanders M, Chandawarkar RY (2006) In vivo delivery of heat shock protein 70 accelerates wound healing by up-regulating macrophage-mediated phagocytosis. Wound Repair Regen 14:129–137
Gordon CB, Li DG, Stagg CA, Manson P, Uldelsman R (1994) Impaired wound healing in Cushing’s syndrome: the role of heat shock proteins. Surgery 116:1082–1087
Author information
Authors and Affiliations
Corresponding author
Additional information
Masaru Odashima and Michiro Otaka are equally contributed to the study
Rights and permissions
About this article
Cite this article
Odashima, M., Otaka, M., Matsuhashi, T. et al. Mechanical Strain Stress Suppresses Expression of HSP70 and Wound Restoration in Gastric Mucosal Cells. Dig Dis Sci 52, 3087–3091 (2007). https://doi.org/10.1007/s10620-007-9795-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10620-007-9795-3