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The Expression of Msi-1 and Its Significance in Small Intestinal Mucosa Severely Damaged by High-Dose 5-FU

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Abstract

Objective The purpose was to investigate the expression of musashi-1 (msi-1) and its significances in small intestinal mucosa that was severely damaged by high-dose 5-FU. Methods A total of 40 adult C57BL/6J mice were divided into two groups: the control group (n = 8, group A) and experimental group (n = 32). The mice in the control group were treated with PBS by intraperitoneal injection, and the other mice were treated with high-dose 5-FU (150 mg/kg body weight for 5 consecutive days) by intraperitoneal injection. At the 1st (group B), 3rd (group C) and 5th (group D) day after treatment with high-dose 5-FU, the dying mice were killed, HE staining and immunohistochemical techniques were used to detect the expression of the putative marker of intestinal epithelial stem cells, msi-1, in samples of the middle intestine from these mice, and the percentage of the msi-1-positive cells from the intestinal mucosal cells of the mice in group B was detected by FACS. Results After treatment with high-dose 5-FU, the intestinal mucosa suffered severe damage: the villi and crypts disappeared, the number of msi-1-positive cells increased greatly, the intestinal epithelial cells could be divided into two fractions by FACS, and the percentage of msi-1-positive cells was up to 67.75% in the fraction in which the value of FSC was higher. Conclusions After treatment with high-dose 5-FU, the percentage of intestinal stem cells had increased significantly, which was useful for the further isolation and enrichment of intestinal epithelial stem cells.

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Acknowledgement

This study was supported by the Natural Science Foundation of Guangdong Province (032901).

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Authors and Affiliations

  1. Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China

    Luo Yuqi, Wu Chengtang, Wen Ying, Lei Shangtong & Liao Kangxiong

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  1. Luo Yuqi
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  2. Wu Chengtang
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  4. Lei Shangtong
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  5. Liao Kangxiong
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Correspondence to Wu Chengtang.

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Yuqi, L., Chengtang, W., Ying, W. et al. The Expression of Msi-1 and Its Significance in Small Intestinal Mucosa Severely Damaged by High-Dose 5-FU. Dig Dis Sci 53, 2436–2442 (2008). https://doi.org/10.1007/s10620-007-0155-0

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  • DOI: https://doi.org/10.1007/s10620-007-0155-0

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