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Dendritic Cells and Toll-Like Receptors 2 and 4 in the Ileum of Crohn's Disease Patients

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Abstract

We investigated myeloid-dendritic cell (DC) marker and Toll-like receptor (TLR)-2 and 4 distributions in ileal samples from Crohn's disease (CD) patients (n = 14) and controls (n = 13). In controls, no TLR-2+ cells were observed, and higher numbers of TLR-4+ and DC-SIGN+ cells (P < 0.01) were detected in ileal samples when compared versus colonic tissues. In non-inflamed CD ileum, TLR-4+ and DC-SGN+ cells were depleted from superficial areas of the villus, and a significant CD1a+ cell infiltration (P < 0.01) was observed when compared to ileal controls and non-inflamed colonic CD samples. In inflamed CD ileum, DC-SIGN+, CD1a+, TLR-4+ and few TLR-4+DC-SIGN+ cells were detected as well as CD83 depletion. No correlation between TLR-2 and DC markers was detected in CD samples. A unique distribution of myeloid-DC markers characterized the CD ileum. Also, the presence of significant amounts of ileal CD1a+ cells may provide a relevant DC-mediated mechanism for antigen recognition in the pathogenesis of CD.

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Authors and Affiliations

  1. Intestinal Disease Research Programme, Department of Pathology and Molecular Medicine, McMaster University, Health Science Centre 3N5C, 1200 Main St. West, Hamilton, ON, Canada, L8N 3Z5

    Manuel A. Silva & Mary H. Perdue

  2. Gastroenterology Division, Internal Medicine Department, University of Chile Clinical Hospital and Clínica Las Condes, Santiago, Chile

    Rodrigo Quera & Jorge Valenzuela

  3. Department of Biomedicine and Surgery, Division of Surgery, University Hospital, Linköping, Sweden

    Sa’ad Y. Salim & Johan D. Söderholm

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  1. Manuel A. Silva
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  2. Rodrigo Quera
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  3. Jorge Valenzuela
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  4. Sa’ad Y. Salim
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  5. Johan D. Söderholm
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  6. Mary H. Perdue
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Correspondence to Manuel A. Silva.

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Silva, M.A., Quera, R., Valenzuela, J. et al. Dendritic Cells and Toll-Like Receptors 2 and 4 in the Ileum of Crohn's Disease Patients. Dig Dis Sci 53, 1917–1928 (2008). https://doi.org/10.1007/s10620-007-0105-x

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  • DOI: https://doi.org/10.1007/s10620-007-0105-x

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