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Anti-Saccharomyces cerevisiae Antibodies in Primary Biliary Cirrhosis

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Abstract

Aim The aim of this study was to evaluate, retrospectively, the frequency of anti-Saccharomyces cerevisiae antibodies (ASCA) in patients with primary biliary cirrhosis (PBC). Methods ASCA, IgG, and IgA, were determined by ELISA in sera of 95 PBC patients; 80 healthy blood donors served as controls. Results The frequency of ASCA (IgG or IgA) was significantly higher in PBC patients than in the control group (24.2% vs 3.7%, P = 0.0001). The frequency of ASCA IgG and ASCA IgA in PBC patients was also significantly higher than that found in the control group (18.9% vs 2.5%, P = 0.0006 and 11.6% vs 1.2%, P = 0.007, respectively). Six patients out of 95 (6.3%) had both ASCA IgG and ASCA IgA; in contrast, none of the control group had both isotypes (P = 0.02). There was no correlation between ASCA levels and mitochondrial autoantibodies (AMA) titres in PBC patients. Conclusion We conclude that ASCA are common in patients with PBC.

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References

  1. Kaplan MM, Gershwin ME (2005) Primary biliary cirrhosis. N Engl J Med 353:1261–1273

    Article  PubMed  CAS  Google Scholar 

  2. Quinton JF, Sendid B, Reumaux D, Duthilleul P, Cortot A, Grandbastien B, Charrier G, Targan SR, Colombel JF, Poulain D (1998) Anti-Saccharomyces cerevisiae mannan antibodies combined with antineutrophil cytoplasmic autoantibodies in inflammatory bowel disease: prevalence and diagnostic role. Gut 42:788–791

    Article  PubMed  CAS  Google Scholar 

  3. Toumi D, Mankaï A, Belhadj R, Ghedira-Besbes L, Jeddi M, Ghedira I (2007) Anti-Saccharomyces cerevisiae antibodies in coeliac disease. Scand J Gastroenterol 42:821–826

    Article  PubMed  CAS  Google Scholar 

  4. Mallant-Hent RCh, Mary B, von Blomberg E, Yuksel Z, Wahab PJ, Gundy C, Meyer GA, Mulder CJ (2006) Disappearance of anti-Saccharomyces cerevisiae antibodies in coeliac disease during a gluten-free diet. Eur J Gastroenterol Hepatol 18:75–78

    Article  PubMed  Google Scholar 

  5. Granito A, Zauli D, Muratori P, Muratori L, Grassi A, Bortolotti R, Petrolini N, Veronesi L, Gionchetti P, Bianchi FB, Volta U (2005) Anti-Saccharomyces cerevisiae and perinuclear anti-neutrophil cytoplasmic antibodies in coeliac disease before and after gluten-free diet. Aliment Pharmacol Ther 21:881–887

    Article  PubMed  CAS  Google Scholar 

  6. Candelli M, Nista EC, Carloni E, Pignataro G, Rigante D, Gasbarrini A (2003) Anti-Saccharomyces cerevisiae antibodies and coeliac disease. Scand J Gastroenterol 38:1191–1192

    Article  PubMed  CAS  Google Scholar 

  7. Damoiseaux JG, Bouten B, Linders AM, Austen J, Roozendaal C, Russel MG, Forget PP, Tervaert JW (2002) Diagnostic value of anti-Saccharomyces cerevisiae and antineutrophil cytoplasmic antibodies for inflammatory bowel disease: high prevalence in patients with celiac disease. J Clin Immunol 22:281–288

    Article  PubMed  CAS  Google Scholar 

  8. Reddy KR, Colombel JF, Poulain D, Krawitt EL (2001) Anti-Saccharomyces cerevisiae antibodies in autoimmune liver disease. Am J Gastroenterol 96:252–253

    Article  PubMed  CAS  Google Scholar 

  9. Muratori P, Muratori L, Guidi M, Maccariello S, Pappas G, Ferrari R, Gionchetti P, Campieri M, Bianchi FB (2003) Anti-Saccharomyces cerevisiae antibodies (ASCA) and autoimmune liver diseases. Clin Exp Immunol 132:473–476

    Article  PubMed  CAS  Google Scholar 

  10. Czaja AJ, Shums Z, Donaldson PT, Norman GL (2004) Frequency and significance of antibodies to Saccharomyces cerevisiae in autoimmune hepatitis. Dig Dis Sci 49:611–618

    Article  PubMed  CAS  Google Scholar 

  11. Krause I, Monselise Y, Milo G, Weinberger A (2002) Anti-Saccharomyces cerevisiae antibodies a novel serologic marker for Behcet’s disease. Clin Exp Rheumatol 20(4 Suppl 26):S21–S24

    PubMed  CAS  Google Scholar 

  12. Torok HP, Glas J, Gruber R, Brumberger V, Strasser C, Kellner H, Marker-Hermann E, Folwaczny C (2004) Inflammatory bowel disease-specific autoantibodies in HLA-B27-associated spondyloarthropathies: increased prevalence of ASCA and pANCA. Digestion 70:49–54

    Article  PubMed  Google Scholar 

  13. Makharia GK, Sachdev V, Gupta R, Lal S, Pandey RM (2007) Anti-saccharomyces cerevisiae antibody does not differentiate between Crohn’s disease and intestinal tuberculosis. Dig Dis Sci 52:33–39

    Article  PubMed  Google Scholar 

  14. Bargou I, Mankaï A, Jamaa A, Ben Jazia I, Skandrani K, Sfar H, Baccouche A, Ajmi S, Letaief A, Fabien N, Jeddi M, Ghedira I (2007) Detection of M2 antimitochondrial antibodies by dot blot assay is more specific than by enzyme linked immunosorbent assay. Pathol Biol (Paris) (in press) doi:10.1016/j.patbio2007.05.001

  15. D’Inca R, Annese V, di Leo V, Latiano A, Quaino V, Abazia C, Vettorato MG, Sturniolo GC (2006) Increased intestinal permeability and NOD2 variants in familial and sporadic Crohn’s disease. Aliment Pharmacol Ther 23:1455–1461

    Article  PubMed  CAS  Google Scholar 

  16. Duerksen DR, Wilhelm-Boyles C, Parry DM (2005) Intestinal permeability in long-term follow-up of patients with celiac disease on a gluten-free diet. Dig Dis Sci 50:785–790

    Article  PubMed  CAS  Google Scholar 

  17. Fresko I, Hamuryudan V, Demir M, Hizli N, Sayman H, Melikoglu M, Tunc R, Yurdakul S, Yazici H (2001) Intestinal permeability in Behcet’s syndrome. Ann Rheum Dis 60:65–66

    Article  PubMed  CAS  Google Scholar 

  18. Vaile JH, Meddings JB, Yacyshyn BR, Russell AS, Maksymowych WP (1999) Bowel permeability and CD45RO expression on circulating CD20+ B cells in patients with ankylosing spondylitis an their relatives. J Rheumatol 26:128–135

    PubMed  CAS  Google Scholar 

  19. Di Leo V, Venturi C, Baragiotta A, Martines D, Floreani A (2003) Gastroduodenal and intestinal permeability in primary biliary cirrhosis. Eur J Gastroenterol Hepatol 15:967–973

    Article  PubMed  Google Scholar 

  20. Feld JJ, Meddings J, Heathcote EJ (2006) Abnormal intestinal permeability in primary biliary cirrhosis. Dig Dis Sci 51:1607–1613

    Article  PubMed  Google Scholar 

  21. Teoh KL, Mackay IR, Rowley MJ, Fussey SP (1994) Enzyme inhibitory autoantibodies to pyruvate dehydrogenase complex in primary biliary cirrhosis differ for mammalian, yeast and bacterial enzymes: implications for molecular mimicry. Hepatology 19:1029–1033

    Article  PubMed  CAS  Google Scholar 

  22. Cabiscol E, Piulats E, Echave P, Herrero E, Ros J (2000) Oxidative stress promotes specific protein damage in Saccharomyces cerevisiae. J Biol Chem 275:27393–27398

    PubMed  CAS  Google Scholar 

  23. Fasano A (2006) Systemic autoimmune disorders in celiac disease. Curr Opin Gastroenterol 22:674–679

    Article  PubMed  Google Scholar 

  24. Fasano A, Shea-Donohue T (2005) Mechanisms of disease: the role of intestinal barrier function in the pathogenesis of gastrointestinal autoimmune diseases. Nat Clin Pract Gastroenterol Hepatol 2:416–422

    Article  PubMed  CAS  Google Scholar 

  25. Watts T, Berti I, Sapone A, Gerarduzzi T, Not T, Zielke R, Fasano A (2005) Role of the intestinal tight junction modulator zonulin in the pathogenesis of type I diabetes in BB diabetic-prone rats. Proc Natl Acad Sci 102:2916–2921

    Article  PubMed  CAS  Google Scholar 

  26. Sendid B, Quinton JF, Charrier G, Goulet O, Cortot A, Grandbastien B, Poulain D, Colombel JF (1998) Anti-Saccharomyces cerevisiae mannan antibodies in familial Crohn’s disease. Am J Gastroenterol 93:1306–1310

    Article  PubMed  CAS  Google Scholar 

  27. Monselise A, Weinberger A, Monselise Y, Fraser A, Sulkes J, Krause I (2006) Anti-Saccharomyces cerevisiae antibodies in Behcet’s disease—a familial study. Clin Exp Rheumatol 24(5 Suppl 42):S087–S090

    Google Scholar 

Download references

Acknowledgement

This study is supported by the Research Unit of Auto-immunity and Allergy (03UR/07-02), Faculty of Pharmacy, Monastir, Tunisia.

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Authors and Affiliations

  1. Research Unit of Auto-immunity and Allergy (03UR/07-02), Faculty of Pharmacy, University of Monastir, Monastir, Tunisia

    Wahiba Sakly, Moncef Jeddi & Ibtissem Ghedira

  2. Laboratory of Immunology, Farhat Hached Hospital, Avenue Ibn El Jazzar, Sousse, 4000, Tunisia

    Ibtissem Ghedira

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  1. Wahiba Sakly
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  2. Moncef Jeddi
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Correspondence to Ibtissem Ghedira.

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Sakly, W., Jeddi, M. & Ghedira, I. Anti-Saccharomyces cerevisiae Antibodies in Primary Biliary Cirrhosis. Dig Dis Sci 53, 1983–1987 (2008). https://doi.org/10.1007/s10620-007-0092-y

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