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Genomic Implications of H2O2 for Cell Proliferation and Growth of Caco-2 Cells

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Abstract

Evidence indicates that oxidative stress inhibits cell proliferation in several cell systems. To determine whether the proliferation of Caco-2 cells is inhibited by oxidative stress and to identify any novel key regulatory factors involved in protecting or damaging the intestine from oxidative stress, Caco-2 cells were treated with an oxidizing agent and analyzed by transcriptomic oligonucleotide microarrays. Results indicated that expression of genes involved in cell proliferation and growth, including genes involved in lipid synthesis, cell cycle progression and cell division, angiogenesis, RNA processing and translation, cAMP metabolism, cytoskeleton and cell to cell adhesion, receptor tyrosine kinases, and intracellular and extracellular signaling, were repressed. If an oxidant-induced inhibition in cell proliferation is involved in the pathogenesis of intestinal disease, information gained could help explain the mechanisms contributing to the causes and consequences of intestinal disease and could aid in the elucidation of mechanisms by which intestinal cells protect against oxidative stress.

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Acknowledgments

This study is published as Journal Series No. 15123, Nebraska Agricultural Research Division, Department of Food Science and Technology, University of Nebraska—Lincoln. The work was supported by the Agricultural Research Division of the University of Nebraska—Lincoln and NIH Grants DK 60447 and DK 063945.

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Authors and Affiliations

  1. Department of Nutrition and Health Sciences, University of Nebraska—Lincoln, Lincoln, Nebraska, 68583, USA

    Theresa A. Herring & Janos Zempleni

  2. Department of Food Science and Technology, University of Nebraska—Lincoln, Lincoln, Nebraska, 68583, USA

    Susan L. Cuppett

  3. 110 RLH, University of Nebraska—Lincoln, Lincoln, Nebraska, 68583-0806, USA

    Theresa A. Herring

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  1. Theresa A. Herring
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Correspondence to Theresa A. Herring.

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Herring, T.A., Cuppett, S.L. & Zempleni, J. Genomic Implications of H2O2 for Cell Proliferation and Growth of Caco-2 Cells. Dig Dis Sci 52, 3005–3015 (2007). https://doi.org/10.1007/s10620-006-9663-6

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  • DOI: https://doi.org/10.1007/s10620-006-9663-6

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