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Oxidative DNA Damage and Antioxidant Activity in Patients with Inflammatory Bowel Disease

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Abstract

Chronic inflammation may contribute to cancer risk through the accumulation of specific products as a result of DNA damage. Endogenous antioxidant enzymes prevent the formation of these harmful products. Oxidative DNA damage and endogenous antioxidant defense were determined in patients with inflammatory bowel disease (IBD). Plasma levels of 8-hydroxydeoxyguanosine (8-OHdG) and nitric oxide (NO) and plasma activities of glutathione peroxidase (G-Px) and superoxide dismutase (SOD) were determined in patients with IBD by ELISA and spectrophotometric assay, respectively. Plasma levels of 8-OHdG, SOD, and G-Px activity were found to be increased in the patient group compared to the control group (P < 0.02, P < 0.001, and P < 0.001, respectively), whereas NO was unchanged. 8-OHdG level was found to be weakly correlated with age, NO, and SOD. The results show increased DNA damage in patients with IBD. This may explain the increased risk of developing colon cancer in these patients.

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Acknowledgements

This work was supported by the Research Fund of Istanbul University (Project number: UDP-550/17062005).

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Authors and Affiliations

  1. Department of Biochemistry, Istanbul University, Dere Sok., Umut Ap. No. 21/44, Sahrayi Cedid, Erenkoy, Istanbul, Turkey

    Yıldız Dincer (Cerrahpasa Medical Faculty), Solen Himmetoglu, Kezban Nur Gunes & Tülay Akcay

  2. Department of Internal Medicine Division of Gastroenterology, Istanbul University, Istanbul, Turkey

    Yusuf Erzin & Kadir Bal (Cerrahpasa Medical Faculty)

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  1. Yıldız Dincer
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  2. Yusuf Erzin
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Correspondence to Yıldız Dincer.

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Dincer, Y., Erzin, Y., Himmetoglu, S. et al. Oxidative DNA Damage and Antioxidant Activity in Patients with Inflammatory Bowel Disease. Dig Dis Sci 52, 1636–1641 (2007). https://doi.org/10.1007/s10620-006-9386-8

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  • DOI: https://doi.org/10.1007/s10620-006-9386-8

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