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Antioxidant Therapy with N-Acetylcysteine Plus Mesalamine Accelerates Mucosal Healing in a Rodent Model of Colitis

  • Inflammatory Bowel Disease
  • Published:
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Abstract

The aims of this study were to examine the ability of the antioxidant N-acetylcysteine (NAC) and mesalamine (5-ASA) alone and in combination to affect TNBS-induced colitis in rat. Three days following induction of TNBS colitis rats were randomized to receive daily intracolonic treatment with NAC, 5-ASA, and NAC plus 5-ASA for 5 or 8 days. At the end of the treatment period macroscopic and microscopic colonic injuries were scored. Myeloperoxidase (MPO) activity and cytokine gene expression were measured in colonic tissues. Results indicated that treatment with NAC plus 5-ASA caused a significantly greater reduction in colonic injury than either agent alone. Furthermore, combination therapy inhibited significantly MPO activity and inflammatory cytokine gene expression in the distal colon of TNBS-treated animals. The beneficial effects of NAC plus 5-ASA on reduction of colonic injury and promotion of healing were most evident after 8 days of treatment.

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Authors and Affiliations

  1. Division of Gastroenterology, Department of Medicine, Oklahoma City, Oklahoma, USA

    Ali Siddiqui MD, Hanumantha Ancha MD, Dustin Tedesco & Richard F. Harty MD

  2. Department of Pathology, University of Oklahoma Health Sciences Center and Oklahoma City Department of Veterans Affairs Medical Center, Oklahoma City, Oklahoma, USA

    Stanley Lightfoot MD

  3. Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA

    Charles A. Stewart PhD

  4. Department of Gastroenterology, Everett Tower, 7 North Pavilion, Room 526, 1200 Everett Drive, Oklahoma City, Oklahoma, 73104, USA

    Richard F. Harty MD

Authors
  1. Ali Siddiqui MD
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  2. Hanumantha Ancha MD
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  3. Dustin Tedesco
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  4. Stanley Lightfoot MD
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  5. Charles A. Stewart PhD
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  6. Richard F. Harty MD
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Corresponding author

Correspondence to Richard F. Harty MD.

Additional information

This work was supported by funds from VA Medical Center, OU Foundation. The authors thank Helen Prince for expert secretarial assistance in manuscript preparation.

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Siddiqui, A., Ancha, H., Tedesco, D. et al. Antioxidant Therapy with N-Acetylcysteine Plus Mesalamine Accelerates Mucosal Healing in a Rodent Model of Colitis. Dig Dis Sci 51, 698–705 (2006). https://doi.org/10.1007/s10620-006-3194-z

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  • DOI: https://doi.org/10.1007/s10620-006-3194-z

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