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Growth Arrest and Decrease of α-SMA and Type I Collagen Expression by Palmitic Acid in the Rat Hepatic Stellate Cell Line PAV-1

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Abstract

Liver fibrosis is characterized by an activation of hepatic stellate cells (HSC). During primary culture HSC evolve from a quiescent into an activated phenotype which is characterized by α-smooth muscle actin (α-SMA) up-regulation, increase in cell growth, and extracellular matrix secretion. HSC culture with trans-resveratrol can lead to deactivation of myofibroblast-like HSC. We used an HSC line, PAV-1, to check the role of retinol and palmitic acid in the deactivation process of HSC. Using mass and metabolic-based methods, Western blot and immunocytochemistry assays, we demonstrated that treatment with palmitic acid (75 μM) alone or in combination with retinol (2 μM) significantly decreased cell proliferation and α-SMA expression. We also established that the association of both compounds strongly decreased collagen type I expression. Our results suggest the potential use of palmitic acid alone or in combination with retinol to induce HSC deactivation.

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Acknowledgment

We thank Alexis Desmoulières (Bordeaux, France) for reviewing the paper.

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Authors and Affiliations

  1. Unité des Maladies Métaboliques et Micro-nutriments, Equipe Vitamines, INRA de Clermont-Fd-Theix, 63122, Saint-Genès, Champanelle, France

    Armand Abergel, Nicolas Dif, Christophe Chassard, Julie Marcand-Sauvant, Edmond Rock & Patrick Sauvant

  2. Centre d’Hépato-Gastro-Entérologie, Hôtel-Dieu, Centre Hospitalo-Universitaire, Boulevard Leon Malfreyt, 63058, Clermont-Fd, France

    Armand Abergel & Patrick Sauvant

  3. UMR INSERM U.384 UA, Faculté de Médecine, Place Henri Dunant, 63000, Clermont-Fd, France

    Vincent Sapin

  4. Laboratoire d’Anatomo-pathologie, Centre Hospitalo-Universitaire, Hôtel-Dieu, 63058, Clermont-Fd, France

    Claude Darcha & Pierre Dechelotte

  5. Laboratoire de Microbiologie, INRA de Clermont-Fd-Theix, 63122, Saint-Genès, Champanelle, France

    Brigitte Gaillard-Martinie

  6. Centre d’Hépato-Gastro-Entérologie, Hôtel-Dieu, Centre Hospitalo-Universitaire, Boulevard Leon Malfreyt, 63058, Clermont-Fd, France

    Armand Abergel

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  1. Armand Abergel
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  2. Vincent Sapin
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  3. Nicolas Dif
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  9. Pierre Dechelotte
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  10. Patrick Sauvant
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Correspondence to Armand Abergel.

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Abergel, A., Sapin, V., Dif, N. et al. Growth Arrest and Decrease of α-SMA and Type I Collagen Expression by Palmitic Acid in the Rat Hepatic Stellate Cell Line PAV-1. Dig Dis Sci 51, 986–995 (2006). https://doi.org/10.1007/s10620-005-9031-y

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