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Uncoupling Protein 2 Involved in Protection of Glucagon–like Peptide 2 in Small Intestine with Ischemia–Reperfusion Injury in Mice

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Abstract

Glucagon-like peptide 2 (GLP-2) is an intestinal epithelium-specific growth factor. However, its protective effects and related mechanism on the small intestine injured by ischemia–reperfusion (I/R) in mice remain unclear. This study aimed to reveal the effects of GLP-2 and its functional relationship with uncoupling protein 2 (UCP2) on the small intestine after I/R injury in mice. Male Balb/c mice were given GLP-2 (250 μg/kg/day, ip) for 3 days and underwent 30 min of superior mesenteric artery occlusion followed by 1 hr of reperfusion on day 4. Histological damage, bacterial translocation, diamine oxidase, and malondialdehyde level were assessed, and UCP2 expression was measured by immunohistochemistry and Western blot. GLP-2 attenuated the intestinal histological damage caused by I/R and increased the villous height by 28% and the crypt depth by 10%, respectively. Compared to the I/R group, diamine oxidase activity was increased, the incidence of bacterial translocation and malondialdehyde level were decreased, and UCP2 expression was increased in GLP-2-treated mice. GLP-2 protected the small intestine from I/R injury and increased UCP2 expression. These results suggested that effects of GLP-2 should be related to the upregulation of mitochondrial UCP2, which antagonized reactive oxygen species production.

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Authors and Affiliations

  1. Department of Physiology, Dalian Medical University, Dalian, 116027, China

    Lili Guan PhD, Dezheng Gong PhD, Nan Tian PhD & Yuan Zou PhD

  2. Department of Physiology, Dalian Medical University, Zhongshan Road 465, Dalian, 116027, LN, China

    Yuan Zou PhD

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  1. Lili Guan PhD
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  2. Dezheng Gong PhD
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  3. Nan Tian PhD
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  4. Yuan Zou PhD
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Correspondence to Yuan Zou PhD.

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Guan, L., Gong, D., Tian, N. et al. Uncoupling Protein 2 Involved in Protection of Glucagon–like Peptide 2 in Small Intestine with Ischemia–Reperfusion Injury in Mice. Dig Dis Sci 50, 554–560 (2005). https://doi.org/10.1007/s10620-005-2474-3

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  • DOI: https://doi.org/10.1007/s10620-005-2474-3

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