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Isolation and characteristics of highly active α-amylase from Bacillus subtilis-150

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Abstract

Partially purified enzyme preparation with specific activities of 153.7 U/mg for α-amylase and 0.15 U/mg for protease was produced by selective adsorption on starch. Enzymes were purified until homogeneous electrophoretically by gel-filtration over HW-55 TSK-gel with specific activities of 245 U/mg for α-amylase and 1.44 U/mg for protease. The optimum temperature and pH for purified α-amylase activity are 40–50°C and pH 6.0. The effects of various metal ions on the activity and stability of the enzyme were studied.

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Authors and Affiliations

  1. Institute of Microbiology, Academy of Sciences of the Republic of Uzbekistan, 100128, Tashkent, ul. A. Kadyri, 7b

    K. T. Normurodova, Sh. Kh. Nurmatov, B. Kh. Alimova, O. M. Pulatova, Z. R. Akhmedova & A. A. Makhsumkhanov

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  1. K. T. Normurodova
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  2. Sh. Kh. Nurmatov
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  3. B. Kh. Alimova
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  4. O. M. Pulatova
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  5. Z. R. Akhmedova
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  6. A. A. Makhsumkhanov
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Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 374–376, July–August, 2007.

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Normurodova, K.T., Nurmatov, S.K., Alimova, B.K. et al. Isolation and characteristics of highly active α-amylase from Bacillus subtilis-150. Chem Nat Compd 43, 454–457 (2007). https://doi.org/10.1007/s10600-007-0159-1

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  • DOI: https://doi.org/10.1007/s10600-007-0159-1

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