Abstract
Panax ginseng, commonly known as Korean ginseng, is a valued source of herbal medicine in Korea and China. We have developed and characterized 35 microsatellite markers in P. ginseng from available BAC end sequences. Characterization of these 35 SSR primer pairs in 14 cultivars of P. ginseng showed 12 primer pairs to be polymorphic and 19 primer pairs to be monomorphic, while the remaining four primer pairs did not produce any product. The number of alleles amplified ranged from 4 to 8 per primer pair, with an average of six alleles per locus. The expected and observed heterozygosities ranged from 0.7500 to 0.9678 and 0.5645 to 0.7109, respectively. None of these loci deviated from Hardy–Weinberg equilibrium. All of the functional primer pairs of P. ginseng showed cross-species transferability with Panax quinquefolium. The cross-species transferable markers could be used for ginseng cultivar identification, for genomic studies, including mapping of specific trait QTL/genes, and for measuring conservation of ginseng.
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References
Fushimi H, Komatsu K, Isobe M, Namba T (1997) Application of PCR-RFLP and MASA analyses on 18S ribosomal RNA gene sequence for the identification of three ginseng drugs. Biol Pharm Bull 20:765–769
Ha WY, Shaw PC, Liu J, Yau FC, Wang J (2002) Authentication of Panax ginseng and Panax quinquefolius using amplified fragment length polymorphism (AFLP) and directed amplification of minisatellite region DNA (DAMD). J Agric Food Chem 50:1871–1875. doi:10.1021/jf011365l
Hong CP, Lee SJ, Park JY, Plaha P, Park YS, Lee YK, Choi JE, Kim KY, Lee JH, Lee J, Jin H, Choi SR, Lim YP (2004) Construction of a BAC library of Korean ginseng and initial analysis of BAC-end sequences. Mol Genet Genomics 276(6):709–716
Kim J, Jo BH, Lee KL, Yoon ES, Ryu GH, Chung KW (2007) Identification of new microsatellite markers in Panax ginseng. Mol Cells 24:60–68
Lim YP, Shin CS, Lee SJ, Youn YN, Jo JS (1993) Survey of proper primers and genetic analysis of Korean ginseng (Panax ginseng C.A. Meyer) variants using the RAPD technique. Korean J Ginseng Sci 17:153–158
Liu K, Muse SV (2005) PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128–2129. doi:10.1093/bioinformatics/bti282
Ma KH, Dixit A, Kim YC, Lee DY, Kim TS, Cho EG, Park YJ (2007) Development and characterization of new micro-satellite markers for ginseng (Panax ginseng C. A. Meyer). Conserv Genet 8:1507–1509. doi:10.1007/s10592-007-9284-4
Mahady GB, Gyllenhaal C, Fong HHS, Farnsworth NR (2000) Ginseng: a review of safety and efficacy. Nutr Clin Care 3(2):90–101
Mihalov JJ, Marderosian AD, Pierce JC (2000) DNA identification of commercial ginseng samples. J Agric Food Chem 48(8):3744–3752. doi:10.1021/jf000011b
Nam KY (2002) Clinical application and efficacy of Korean ginseng (Panax ginseng C.A. Meyer). J Ginseng Res 26:111–131
Ngan F, Shaw P, But P, Wang J (1999) Molecular authentication of Panax species. Phytochemistry 50:787–791. doi:10.1016/S0031-9422(98)00606-2
Volger PK, Pittler MH, Ernst E (1999) The efficacy of ginseng. A systematic review of randomized clinical trials. Eur J Clin Pharmacol 55(8):567–575. doi:10.1007/s002280050674
Acknowledgments
This study was financially supported by research funds from Chungnam National University (2006) and Korea National Plant Research Resource Center (KOSEF, 2009-0062568), Korea.
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Van Dan, N., Ramchiary, N., Choi, S.R. et al. Development and characterization of new microsatellite markers in Panax ginseng (C.A. Meyer) from BAC end sequences. Conserv Genet 11, 1223–1225 (2010). https://doi.org/10.1007/s10592-009-9924-y
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DOI: https://doi.org/10.1007/s10592-009-9924-y